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Abstract:

Brucella spp. are facultative intracellular bacteria pathogenic for many mammalian species including humans, causing a disease called brucellosis. Learning how Brucella adapts to its intracellular niche is crucial for understanding its pathogenesis mechanism, allowing for the development of new and more effective vaccines and treatments against brucellosis. Brucella pathogenesis resides mostly in its ability to adapt to the harsh environmental conditions encountered during host infection such as the oxygen depletion. The mechanism by which Brucella senses the oxygen tension and triggers its environmental adaptation is unknown. In this work we show that the Brucella abortus NtrY/NtrX two-component system is involved in oxygen sensing through a haem group contained in a Per-ARNT-SIM (PAS) domain of the NtrY histidine kinase. The NtrY haem iron can be reduced to the ferrous form and is rapidly oxidized to the ferric form in presence of oxygen. Importantly, we show that the oxidation state of the haem iron modulates the autokinase activity, being the anoxygenic reduced ferrous form the signalling state of NtrY. Also, we show that ntrY gene expression increases under low oxygen tension and that NtrY transfers its signal to its cognate response regulator NtrX, regulating in this way the expression of nitrogen respiration enzymes. Based on these findings, we postulate that NtrY acts as a redox sensor in Brucella spp. © 2012 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes
Autor:Carrica, M.C.; Fernandez, I.; Martí, M.A.; Paris, G.; Goldbaum, F.A.
Filiación:Fundación Instituto Leloir (IIBBA-CONICET), Av. Patricias Argentinas 435, Buenos Aires, Argentina
Departamento de Química Biológica y INQUIMAE-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires Ciudad Universitaria, Pabellón 2, C1428EHA, Buenos Aires, Argentina
Palabras clave:ferric ion; ferrous ion; iron; nitrate reductase; nitric oxide reductase; nitrite reductase; nitrous oxide reductase; oxygen; protein histidine kinase; protein NtrX; protein NtrY; unclassified drug; animal cell; article; bacterial gene; biosensor; Brucella abortus; controlled study; denitrification; enzyme regulation; gene expression; nonhuman; ntrY gene; oxidation reduction state; oxygen tension; priority journal; protein domain; protein expression; regulatory mechanism; signal transduction; Adaptation, Physiological; Animals; Brucella abortus; Cell Line; Denitrification; Gene Expression Regulation, Bacterial; Heme; Mice; Nitrogen; Operon; Oxidation-Reduction; Oxygen; Phosphorylation; Promoter Regions, Genetic; Protein Kinases; Signal Transduction; Brucella; Brucella melitensis biovar Abortus; Mammalia
Año:2012
Volumen:85
Número:1
Página de inicio:39
Página de fin:50
DOI: http://dx.doi.org/10.1111/j.1365-2958.2012.08095.x
Título revista:Molecular Microbiology
Título revista abreviado:Mol. Microbiol.
ISSN:0950382X
CODEN:MOMIE
CAS:ferric ion, 20074-52-6; ferrous ion, 15438-31-0; iron, 14093-02-8, 53858-86-9, 7439-89-6; nitrate reductase, 37256-45-4, 37292-87-8; nitrite reductase, 9080-03-9; nitrous oxide reductase, 55576-44-8; oxygen, 7782-44-7; protein histidine kinase, 99283-67-7; Heme, 14875-96-8; Nitrogen, 7727-37-9; Oxygen, 7782-44-7; Protein Kinases, 2.7.-; protein-histidine kinase, 2.7.3.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v85_n1_p39_Carrica

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Citas:

---------- APA ----------
Carrica, M.C., Fernandez, I., Martí, M.A., Paris, G. & Goldbaum, F.A. (2012) . The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes. Molecular Microbiology, 85(1), 39-50.
http://dx.doi.org/10.1111/j.1365-2958.2012.08095.x
---------- CHICAGO ----------
Carrica, M.C., Fernandez, I., Martí, M.A., Paris, G., Goldbaum, F.A. "The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes" . Molecular Microbiology 85, no. 1 (2012) : 39-50.
http://dx.doi.org/10.1111/j.1365-2958.2012.08095.x
---------- MLA ----------
Carrica, M.C., Fernandez, I., Martí, M.A., Paris, G., Goldbaum, F.A. "The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes" . Molecular Microbiology, vol. 85, no. 1, 2012, pp. 39-50.
http://dx.doi.org/10.1111/j.1365-2958.2012.08095.x
---------- VANCOUVER ----------
Carrica, M.C., Fernandez, I., Martí, M.A., Paris, G., Goldbaum, F.A. The NtrY/X two-component system of Brucella spp. acts as a redox sensor and regulates the expression of nitrogen respiration enzymes. Mol. Microbiol. 2012;85(1):39-50.
http://dx.doi.org/10.1111/j.1365-2958.2012.08095.x