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Abstract:

Intracellular levels of cyclic nucleotide second messengers are regulated predominantly by a large superfamily of phosphodiesterases (PDEs). Trypanosoma cruzi, the causative agent of Chagas disease, encodes four different PDE families. One of these PDEs, T. cruzi PDE C2 (TcrPDEC2) has been characterized as a FYVE domain containing protein. Here, we report a novel role for TcrPDEC2 in osmoregulation in T. cruzi and reveal the relevance of its FYVE domain. Our data show that treatment of epimastigotes with TcrPDEC2 inhibitors improves their regulatory volume decrease, whereas cells overexpressing this enzyme are unaffected by the same inhibitors. Consistent with these results, TcrPDEC2 localizes to the contractile vacuole complex, showing strong labelling in the region corresponding to the spongiome. Furthermore, transgenic parasites overexpressing a truncated version of TcrPDEC2 without the FYVE domain show a failure in its targeting to the contractile vacuole complex and a marked decrease in PDE activity, supporting the importance of this domain to the localization and activity of TcrPDEC2. Taking together, the results here presented are consistent with the importance of the cyclic AMP signalling pathway in regulatory volume decrease and implicate TcrPDEC2 as a specifically localized PDE involved in osmoregulation in T. cruzi. © 2010 Blackwell Publishing Ltd.

Registro:

Documento: Artículo
Título:Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi
Autor:Schoijet, A.C.; Miranda, K.; Medeiros, L.C.S.; De Souza, W.; Flawiá, M.M.; Torres, H.N.; Pignataro, O.P.; Docampo, R.; Alonso, G.D.
Filiación:Instituto de Investigaciones en Ingeniería Genética y Biología Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
Center for Tropical and Emerging Global Diseases, Department of Cellular Biology, University of Georgia, Athens, GA 30602, United States
Lab. de Ultraestrutura Celular Hertha Meyer, Instituto de Biofisica Carlos Chagas Filho, Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, Universidade Federal do Rio de Janeiro, Brazil
Instituto Nacional de Metrologia, Normalização e Qualidade Industrial, INMETRO, Rio de Janeiro, Brazil
Departamento de Fisiología, Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Endocrinología Molecular y Transducción de Señales, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Técnicas, Vuelta de Obligado 2490, C1428ADN Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:cyclic AMP phosphodiesterase; article; cell vacuole; controlled study; enzyme activity; epimastigote; gene overexpression; immunofluorescence microscopy; nonhuman; osmoregulation; priority journal; protein binding; protein domain; protein localization; radioimmunoassay; signal transduction; transgene; Trypanosoma cruzi; 3',5'-Cyclic-AMP Phosphodiesterases; Enzyme Inhibitors; Gene Expression; Microscopy, Immunoelectron; Protein Structure, Tertiary; Trypanosoma cruzi; Vacuoles; Water-Electrolyte Balance; Trypanosoma cruzi
Año:2011
Volumen:79
Número:1
Página de inicio:50
Página de fin:62
DOI: http://dx.doi.org/10.1111/j.1365-2958.2010.07429.x
Título revista:Molecular Microbiology
Título revista abreviado:Mol. Microbiol.
ISSN:0950382X
CODEN:MOMIE
CAS:cyclic AMP phosphodiesterase, 9036-21-9; 3',5'-Cyclic-AMP Phosphodiesterases, 3.1.4.17; Enzyme Inhibitors
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_0950382X_v79_n1_p50_Schoijet.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v79_n1_p50_Schoijet

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Citas:

---------- APA ----------
Schoijet, A.C., Miranda, K., Medeiros, L.C.S., De Souza, W., Flawiá, M.M., Torres, H.N., Pignataro, O.P.,..., Alonso, G.D. (2011) . Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi. Molecular Microbiology, 79(1), 50-62.
http://dx.doi.org/10.1111/j.1365-2958.2010.07429.x
---------- CHICAGO ----------
Schoijet, A.C., Miranda, K., Medeiros, L.C.S., De Souza, W., Flawiá, M.M., Torres, H.N., et al. "Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi" . Molecular Microbiology 79, no. 1 (2011) : 50-62.
http://dx.doi.org/10.1111/j.1365-2958.2010.07429.x
---------- MLA ----------
Schoijet, A.C., Miranda, K., Medeiros, L.C.S., De Souza, W., Flawiá, M.M., Torres, H.N., et al. "Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi" . Molecular Microbiology, vol. 79, no. 1, 2011, pp. 50-62.
http://dx.doi.org/10.1111/j.1365-2958.2010.07429.x
---------- VANCOUVER ----------
Schoijet, A.C., Miranda, K., Medeiros, L.C.S., De Souza, W., Flawiá, M.M., Torres, H.N., et al. Defining the role of a FYVE domain in the localization and activity of a cAMP phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi. Mol. Microbiol. 2011;79(1):50-62.
http://dx.doi.org/10.1111/j.1365-2958.2010.07429.x