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Abstract:

The PhoP/PhoQ two-component system plays an essential role regulating numerous virulence phenotypes in Salmonella enterica. Previous work showed that PhoQ, the sensor protein, switches between the kinase- and the phosphatase-dominant state in response to environmental Mg2+ availability. This switch defines the PhoP phosphorylation status and, as a result, the transcriptional activity of this regulator. In this work, using the FlAsH labelling technique, we examine PhoP cytolocalization in response to extracellular Mg2+ limitation in vitro and to the Salmonella-containing vacuole (SCV) environment in macrophage cells. We demonstrate that in these PhoP/PhoQ-inducing environments PhoP displays preferential localization to one cell pole, while being homogeneously distributed in the bacterial cytoplasm in repressing conditions. Polar localization is lost in the absence of PhoQ or when a non-phosphorylatable PhoPD52A mutant is expressed. However, when PhoP transcriptional activation is achieved in a Mg2+- and PhoQ-independent manner, PhoP regains asymmetric polar localization. In addition, we show that, in the analysed conditions, PhoQ cellular distribution does not parallel PhoP location pattern. These findings reveal that PhoP cellular location is dynamic and conditioned by its environmentally defined transcriptional status, showing a new insight in the PhoP/PhoQ system mechanism. © 2008 The Authors.

Registro:

Documento: Artículo
Título:Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment
Autor:Sciara, M.I.; Spagnuolo, C.; Jares-Erijman, E.; García Véscovi, E.
Filiación:Instituto de Biología Molecular Y Celular de Rosario (IBR-CONICET), Departamento de Microbiología, Universidad Nacional de Rosario, S2002LRK Rosario, Argentina
Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales, CIHIDECAR-CONICET, C1428EHA Buenos Aires, Argentina
Palabras clave:bacterial protein; magnesium ion; mutant protein; PhoP protein; protein phoq; unclassified drug; animal cell; article; cell vacuole; cellular distribution; controlled study; cytoplasm; fluorescence microscopy; in vitro study; macrophage; nonhuman; priority journal; protein expression; protein localization; Salmonella enterica; transcription initiation; Animals; Bacterial Proteins; Cell Line; Cytoplasm; Macrophages; Magnesium; Mice; Microscopy, Fluorescence; Salmonella enterica; Bacteria (microorganisms); Salmonella; Salmonella enterica; Strawberry crinkle virus
Año:2008
Volumen:70
Número:2
Página de inicio:479
Página de fin:493
DOI: http://dx.doi.org/10.1111/j.1365-2958.2008.06427.x
Título revista:Molecular Microbiology
Título revista abreviado:Mol. Microbiol.
ISSN:0950382X
CODEN:MOMIE
CAS:magnesium ion, 22537-22-0; Bacterial Proteins; Magnesium, 7439-95-4; PhoP protein, Bacteria, 125360-99-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v70_n2_p479_Sciara

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Citas:

---------- APA ----------
Sciara, M.I., Spagnuolo, C., Jares-Erijman, E. & García Véscovi, E. (2008) . Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment. Molecular Microbiology, 70(2), 479-493.
http://dx.doi.org/10.1111/j.1365-2958.2008.06427.x
---------- CHICAGO ----------
Sciara, M.I., Spagnuolo, C., Jares-Erijman, E., García Véscovi, E. "Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment" . Molecular Microbiology 70, no. 2 (2008) : 479-493.
http://dx.doi.org/10.1111/j.1365-2958.2008.06427.x
---------- MLA ----------
Sciara, M.I., Spagnuolo, C., Jares-Erijman, E., García Véscovi, E. "Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment" . Molecular Microbiology, vol. 70, no. 2, 2008, pp. 479-493.
http://dx.doi.org/10.1111/j.1365-2958.2008.06427.x
---------- VANCOUVER ----------
Sciara, M.I., Spagnuolo, C., Jares-Erijman, E., García Véscovi, E. Cytolocalization of the PhoP response regulator in Salmonella enterica: Modulation by extracellular Mg2+ and by the SCV environment. Mol. Microbiol. 2008;70(2):479-493.
http://dx.doi.org/10.1111/j.1365-2958.2008.06427.x