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Abstract:

Bacillus subtilis growing at 37° C synthesizes, almost exclusively, saturated fatty acids. However, when a culture growing at 37°C is transferred to 20°C, the synthesis of unsaturated fatty acids is induced. The addition of the DNA gyrase inhibitor novobiocin specifically prevented the induction of unsaturated fatty acid synthesis at 20° C. Furthermore, it was determined that plasmid DNA isolated from cells growing at 20°C was significantly more negatively supercoiled than the equivalent DNA isolated from cells growing at 37°C. The overall results agree with the hypothesis that an increase in DNA supercoiling associated with a temperature downshift could regulate the unsaturated fatty acids synthesis in B. subtilis. Copyright © 1994, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis
Autor:Grau, R.; Gardiol, D.; Glikin, G.C.; De Mendoza, D.
Filiación:Departamento de Microbiologiá, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531, Rosario, 2000, Argentina
Instituto de Investigaciones en Ingenieriá Genética y Biologiá Molecular, Consejo Nacional de Investigaciones Científicas y Técnicas, y Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:novobiocin; unsaturated fatty acid; article; bacillus subtilis; dna supercoiling; fatty acid synthesis; nonhuman; priority journal; temperature; Bacillus subtilis; Bacterial Proteins; DNA Topoisomerases, Type II; DNA, Bacterial; DNA, Superhelical; Fatty Acids, Unsaturated; Gene Expression Regulation, Bacterial; Novobiocin; Support, Non-U.S. Gov't; Temperature; Bacillus subtilis
Año:1994
Volumen:11
Número:5
Página de inicio:933
Página de fin:941
DOI: http://dx.doi.org/10.1111/j.1365-2958.1994.tb00372.x
Título revista:Molecular Microbiology
Título revista abreviado:Mol. Microbiol.
ISSN:0950382X
CAS:Bacterial Proteins; DNA Topoisomerases, Type II, EC 5.99.1.3; DNA, Bacterial; DNA, Superhelical; Fatty Acids, Unsaturated; Novobiocin, 303-81-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0950382X_v11_n5_p933_Grau

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Citas:

---------- APA ----------
Grau, R., Gardiol, D., Glikin, G.C. & De Mendoza, D. (1994) . DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis. Molecular Microbiology, 11(5), 933-941.
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00372.x
---------- CHICAGO ----------
Grau, R., Gardiol, D., Glikin, G.C., De Mendoza, D. "DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis" . Molecular Microbiology 11, no. 5 (1994) : 933-941.
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00372.x
---------- MLA ----------
Grau, R., Gardiol, D., Glikin, G.C., De Mendoza, D. "DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis" . Molecular Microbiology, vol. 11, no. 5, 1994, pp. 933-941.
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00372.x
---------- VANCOUVER ----------
Grau, R., Gardiol, D., Glikin, G.C., De Mendoza, D. DNA supercoiling and thermal regulation of unsaturated fatty acid synthesis in Bacillus subtilis. Mol. Microbiol. 1994;11(5):933-941.
http://dx.doi.org/10.1111/j.1365-2958.1994.tb00372.x