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Abstract:

Sulfated polysaccharides exhibit many biological properties such as antiviral and anticoagulant activities. Herein, we report the antiviral activity of sulfated galactans extracted from the red seaweed Bostrychia montagnei against herpes simplex virus types 1 (strain F and the thymidine kinase-deficient strains Field and B2006) and 2 (strain G). Two crude extracts obtained with cold and hot water as well as some fractions obtained by anion exchange chromatography, inhibited significantly the replication of the different strains of herpesviruses as determined by plaque reduction assays. The inhibitory effect of the compounds studied here took place only when they were added during the adsorption period. They were found to be highly selective antiviral substances, causing no impairment of Vero cell viability. Furthermore, they had no direct inactivating effect on virions by incubation in a virucidal assay. The antiviral activity could be correlated with the molecular weight and sulfate content of the polysaccharides. Although sulfated polysaccharides are generally endowed with anticoagulant properties, the results of the activated partial thromboplastin time and the thrombine time assays indicated that the natural sulfated polysaccharides from Bostrychia montagnei have very low anticoagulant activity, confirming that there is no relation between the antiviral and anticoagulant properties.

Registro:

Documento: Artículo
Título:Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro
Autor:Duarte, M.E.R.; Noseda, D.G.; Noseda, M.D.; Tulio, S.; Pujol, C.A.; Damonte, E.B.
Filiación:Departamento de Bioquímica, Universidade Federal Do Paraná, Centro Politécnico, Curitiba, Paraná, Brazil
Laboratorio de Virología, Departamento de Química Biológica, Ciudad Universitaria, Buenos Aires, Argentina
Palabras clave:Anticoagulant; Antiviral; Herpes simplex virus; Polysaccharides; Seaweed; Sulfated galactans; anticoagulant agent; antivirus agent; galactan; alga; antiviral activity; article; cell viability; herpes; Herpes simplex virus; nonhuman; priority journal; seaweed; thrombosis; virus replication; algae; Bostrychia montagnei; Bostrychia montagnei; Herpes; Simplexvirus
Año:2001
Volumen:8
Número:1
Página de inicio:53
Página de fin:58
DOI: http://dx.doi.org/10.1078/0944-7113-00007
Título revista:Phytomedicine
Título revista abreviado:Phytomedicine
ISSN:09447113
CODEN:PYTOE
CAS:galactan, 39300-87-3, 9037-55-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09447113_v8_n1_p53_Duarte

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Citas:

---------- APA ----------
Duarte, M.E.R., Noseda, D.G., Noseda, M.D., Tulio, S., Pujol, C.A. & Damonte, E.B. (2001) . Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro. Phytomedicine, 8(1), 53-58.
http://dx.doi.org/10.1078/0944-7113-00007
---------- CHICAGO ----------
Duarte, M.E.R., Noseda, D.G., Noseda, M.D., Tulio, S., Pujol, C.A., Damonte, E.B. "Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro" . Phytomedicine 8, no. 1 (2001) : 53-58.
http://dx.doi.org/10.1078/0944-7113-00007
---------- MLA ----------
Duarte, M.E.R., Noseda, D.G., Noseda, M.D., Tulio, S., Pujol, C.A., Damonte, E.B. "Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro" . Phytomedicine, vol. 8, no. 1, 2001, pp. 53-58.
http://dx.doi.org/10.1078/0944-7113-00007
---------- VANCOUVER ----------
Duarte, M.E.R., Noseda, D.G., Noseda, M.D., Tulio, S., Pujol, C.A., Damonte, E.B. Inhibitory effect of sulfated galactans from the marine alga Bostrychia montagnei on herpes simplex virus replication in vitro. Phytomedicine. 2001;8(1):53-58.
http://dx.doi.org/10.1078/0944-7113-00007