Magdaleno, A.; Peralta Gavensky, M.; Fassiano, A.V.; Ríos de Molina, M.C.; Santos, M.; March, H.; Moretton, J.; Juárez, Á.B. "Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays" (2015) Environmental Science and Pollution Research. 22(23):19194-19202
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The imazethapyr herbicide (formulation Verosil ® ) was evaluated for phytotoxicity and genotoxicity using a battery of bioassays: (1) the growth inhibition of the green alga Pseudokirchneriella subcapitata, (2) the root growth and germination of the higher plant Lactuca sativa, (3) the genetic damage using the Salmonella/microsome test, and (4) the aneugenic and clastogenic effects on Allium cepa. The Verosil ® formulation was highly toxic to the non-target green alga (median effective concentration (EC50) = 1.05 ± 0.05 mg active ingredient (a.i.) L −1 ), and concentrations above 10 mg a.i. L −1 inhibited root elongation in lettuce: relative growth index (RGI) between 0.28 ± 0.01 and 0.66 ± 0.10. No genotoxic effect was observed in Salmonella typhimurium at 100 mg a.i. L −1 , either with or without the microsomal fraction. However, significant differences in the frequency of chromosomal aberrations in anaphases and telophases (bridges, chromosome fragments, and vagrants) were observed in A. cepa at concentrations between 0.01 and 1 mg a.i. L −1 with respect to the control. The frequencies of micronuclei showed significant differences with respect to the control at concentrations between 0.001 and 0.1 mg a.i. L −1 . A very high mitotic index (MI = 93.8 ± 5.8) was observed associated with a high number of cells in the prophase stage at 100 mg a.i. L −1 , indicating cytotoxicity. These results showed that imazethapyr is toxic to the non-target populations in both aquatic and terrestrial ecosystems. This herbicide might also exert clastogenic and aneugenic mitotic damage in higher plants. Therefore, the imazethapyr formulation may constitute an environmental risk to plants. © 2015, Springer-Verlag Berlin Heidelberg.


Documento: Artículo
Título:Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays
Autor:Magdaleno, A.; Peralta Gavensky, M.; Fassiano, A.V.; Ríos de Molina, M.C.; Santos, M.; March, H.; Moretton, J.; Juárez, Á.B.
Filiación:Cátedra de Salud Pública e Higiene Ambiental, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, 4° Piso, Buenos Aires, C1113AAC, Argentina
Departamento de Química Biológica, IQUIBICEN UBA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, IBBEA UBA-CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto Nacional de Tecnología Industrial (INTI), San Martín, Buenos Aires, Argentina
Agrofina S.A., Vicente López, Buenos Aires, Argentina
Palabras clave:Allium cepa; Ames test; Genotoxicity; Imazethapyr; Lactuca sativa; Pseudokirchneriella subcapitata; Toxicity; bioassay; chromosome; concentration (composition); environmental risk; genotoxicity; germination; green alga; growth response; herb; imazethapyr; inhibition; leafy vegetable; phytotoxicity; Allium cepa; Chlorophyta; Embryophyta; Lactuca; Lactuca sativa; Salmonella typhimurium; Selenastrum capricornutum; herbicide; imazethapyr; mutagenic agent; nicotinic acid derivative; water pollutant; bioassay; chromosome aberration; DNA damage; drug effects; genetics; green alga; growth, development and aging; lettuce; microbial sensitivity test; micronucleus test; mitosis; mitosis index; onion; plant root; Salmonella enterica serovar Typhimurium; toxicity; water pollutant; Biological Assay; Chlorophyta; Chromosome Aberrations; DNA Damage; Herbicides; Lettuce; Microbial Sensitivity Tests; Micronucleus Tests; Mitosis; Mitotic Index; Mutagens; Nicotinic Acids; Onions; Plant Roots; Salmonella typhimurium; Water Pollutants, Chemical
Página de inicio:19194
Página de fin:19202
Título revista:Environmental Science and Pollution Research
Título revista abreviado:Environ. Sci. Pollut. Res.
CAS:Herbicides; imazethapyr; Mutagens; Nicotinic Acids; Water Pollutants, Chemical


  • Abreu, M.M., Lopes, J., Santos, E.S., Magalhães, M.C.F., Ecotoxicity evaluation of an amended soil contaminated with uranium and radium using sensitive plants (2014) J Geochem Explor, 142, pp. 112-121. , COI: 1:CAS:528:DC%2BC2cXisFOmu7Y%3D
  • Archibald, P.A., Bold, H.C., (1970) Phycological studies. XI. The genus Chlorococcum meneghini, , Univ Texas, Austin: 86 p, Public N° 7015
  • Avigliano, L., Fassiano, A.V., Medesani, D.A., Ríos de Molina, M.C., Rodríguez, E.M., Effects of glyphosate on growth rate, metabolic rate and energy reserves of early juvenile crayfish, Cherax quadricarinatus M (2014) Bull Environ Contam Toxicol, 92 (6), pp. 631-635. , COI: 1:CAS:528:DC%2BC2cXjtl2msLk%3D
  • Battaglin, W.A., Furlong, E.T., Burkhardt, M.R., Peter, C.J., Occurrence of sulfonylurea, sulfonamide, imidazolinone, and other herbicides in Rivers, reservoirs and ground water in the Midwestern United States, 1998 (2000) Sci Total Environ, 248, pp. 123-133. , COI: 1:CAS:528:DC%2BD3cXitlGrsrY%3D
  • Blaise, C., Férard, J.F., Vasseur, P., Microplate toxicity tests with microalgae: a review (1998) Microscale testing in aquatic toxicology. Advances, techniques, and practice, pp. 269-288. , Wells P, Lee K, Blaise C, (eds), CRC, Boca Raton
  • Bowers, N., Pratt, J.R., Beeson, D., Lewis, M., Comparative evaluation of soil toxicity using lettuce seeds and soil ciliates (1997) Environ Toxicol Chem, 16, pp. 207-213. , COI: 1:CAS:528:DyaK2sXhsFahsr4%3D
  • Charles, J., Sancey, B., Morin-Crini, N., Badot, P.M., Degiorgi, F., Trunfio, G., Crini, G., Evaluation of the phytotoxicity of polycontaminated industrial effluents using the lettuce plant (Lactuca sativa) as a bioindicator (2011) Ecotoxicol Environ Saf, 74, pp. 2057-2064. , COI: 1:CAS:528:DC%2BC3MXht1ehs7nM
  • Technical guidance document in support of Commission Directive 93/67/EEC on risk assessment for new notified substances and Commission Regulation (EC) No 1488/94 on risk assessment for existing substances. Part II (1996) Environmental risk assessment, , Office for Official Publications of the European Communities, Luxembourg
  • Dutka, B., Short-term root elongation toxicity bioassay. Methods for toxicological analysis of waters, wastewaters and sediments (1989) National Water Research Institute (NWRI), , Environment Canada, Canada
  • El-Nahas, A.I., Mutagenic potential of imazethapyr herbicide (Persuit*) on Vicia faba in the presence of urea fertilizer (2000) Pak J Biol Sci, 3 (5), pp. 600-905
  • Canada, E., Biological test method: Growth inhibition test using a freshwater algae. EPS 1/RM/25, Environmental Science and Technology Centre, Science and Technology Branch, Environment Canada. Environmental Protection Series, Second Edition (2007) p 53
  • Espy, R., Pelton, E., Opseth, A., Kasprisin, J., Nienow, A.M., Photodegradation of the herbicide imazethapyr in aqueous solution: effects of wavelength, pH, and natural organic matter (NOM) and analysis of photoproducts (2011) J Agric Food Chem, 59, pp. 7277-7285. , COI: 1:CAS:528:DC%2BC3MXnsVertr4%3D
  • Estevam, E.C., Nakano, E., Kawano, T., Pereira, C.A.B., Amancio, F.F., Melo, A.M.M.A., Dominant lethal effects of 2,4-D in Biomphalaria glabrata (2006) Mutat Res, 611, pp. 83-88. , COI: 1:CAS:528:DC%2BD28Xht1eqtbvP
  • Finney, D.J., (1971) Probit analysis, , Cambridge University Press, Cambridge, UK
  • Fiskesjǒ, G., The Allium test as a standard in environmental monitoring (1985) Hereditas, 102, pp. 99-112
  • Fragiorge, E.J., Alves, A., de Rezende, A., Graf, U., Spanó, M.A., Comparative genotoxicity evaluation of imidazolinone herbicides in somatic cells of Drosophila melanogaster (2008) Food Chem Toxicol, 46, pp. 393-401. , COI: 1:CAS:528:DC%2BD2sXhsVCgu7jK
  • Franklin, N.M., Stauber, J.L., Lim, R.P., Petocz, P., Toxicity of metal mixtures to a tropical freshwater alga (Chlorella sp.): the effect of interactions between copper, cadmium, and zinc on metal cell binding and uptake (2002) Environ Toxicol Chem, 21 (11), pp. 2412-2422. , COI: 1:CAS:528:DC%2BD38XotVGntrk%3D
  • Gholami-Seyedkolaei, S.J., Mirvaghefi, A., Farahmand, H., Kosari, A.A., Gholami-Seyedkolaei, S.J., Gholami-Seyedkolaei, S.J., Optimization of recovery patterns in common carp exposed to roundup using response surface methodology: evaluation of neurotoxicity and genotoxicity effects and biochemical parameters (2013) Ecotoxicol Environ Saf, 98, pp. 152-161. , COI: 1:CAS:528:DC%2BC3sXhsFGns7bP
  • Grant, W.F., Chromosome aberration assays in Allium. A report of the US Environmental Protection Agency Gene-Tox Program (1982) Mutat Res, 99, pp. 273-291. , COI: 1:CAS:528:DyaL38XmtV2ks70%3D
  • Grant, W.F., Higher plant assays for the detection of chromosomal aberrations and gene mutation—a brief historical background on their use for screening and monitoring environmental chemicals (1999) Mutat Res, 426, pp. 107-112. , COI: 1:CAS:528:DyaK1MXjsVSgs78%3D
  • Grant, W.F., Owens, E.T., Zea mays assays of chemical/radiation genotoxicity for the study of environmental mutagens (2006) Mutat Res, 613 (1), pp. 17-64. , COI: 1:CAS:528:DC%2BD28XoslOns78%3D
  • Grisolia, C.K., Bilich, M.R., Menezes Formigli, L., A comparative toxicologic and genotoxic study of the herbicide arsenal, its active ingredient imazapyr, and the surfactant nonylphenol ethoxylate (2004) Ecotoxicol Environ Saf, 59, pp. 123-126. , COI: 1:CAS:528:DC%2BD2cXlvVWhs70%3D
  • Hoshina, M.M., Marin-Morales, M.A., Micronucleus and chromosome aberrations induced in onion (Allium cepa) by a petroleum refinery effluent and by river water that receives this effluent (2009) Ecotoxicol Environ Saf, 72, pp. 2090-2095. , COI: 1:CAS:528:DC%2BD1MXhtlWgtbzM
  • Kanaya, N., Gill, B.S., Grover, I.S., Murin, A., Osiecka, R., Sandhu, S.S., Anderson, H.C., Vicia faba chromosomal aberration assay (1994) Mutat Res, 310, pp. 231-247. , COI: 1:CAS:528:DyaK2cXmsVyns7s%3D
  • Leme, D.M., Marin-Morales, M.A., Allium cepa test in environmental monitoring: a review on its application (2009) Mutat Res, 682, pp. 71-81. , COI: 1:CAS:528:DC%2BD1MXhtVenu7jM
  • Lewis, M.A., Chronic toxicity of surfactants and detergent builders to algae: a review and risk assessment (1990) Ecotoxicol Environ Saf, 20, pp. 123-140. , COI: 1:CAS:528:DyaK3MXjslaiuw%3D%3D
  • Lewis, M.A., Use of freshwater plants for phytotoxicity testing: a review (1995) Environ Pollut, 87, pp. 319-336. , COI: 1:CAS:528:DyaK2MXivFKru7o%3D
  • Liman, R., Ciğerci, I.H., Öztürk, N.S., Determination of genotoxic effects of Imazethapyr herbicide in Allium cepa root cells by mitotic activity, chromosome aberration, and comet assay (2015) Pestic Biochem Physiol, 118, pp. 38-42. , COI: 1:CAS:528:DC%2BC2cXitVSgu73J
  • Losi-Guembarovski, R., Santos, F.V., Dias, F.L., Frederico, R.G., Cólus, I.M.S., Assessment of the ability of Imazaquin herbicide to induce chromosomal aberrations in vitro in cultured Chinese hamster ovary cells and micronuclei in vivo in mice (2004) Food Chem Toxicol, 42, pp. 1245-1249. , COI: 1:CAS:528:DC%2BD2cXltVKitLY%3D
  • Ma, T.H., Tradescantia micronucleus bioassay and pollen tube chromatid aberrations test for in situ monitoring and mutagen screening (1981) Environ Health Perspect, 37, pp. 85-90. , COI: 1:STN:280:DyaL3M7hsVWjug%3D%3D
  • Magdaleno, A., Gómez, C.E., Vélez, C.G., Accorinti, J., Preliminary toxicity tests using the green alga Ankistrodesmus falcatus (1997) Environ Toxicol Water Qual, 12 (1), pp. 11-14. , COI: 1:CAS:528:DyaK2sXnvFWkuw%3D%3D
  • Magdaleno, A., Saenz, M.E., Juárez, A.B., Moretton, J., Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata (2015) Ecotoxicol Environ Saf, 113, pp. 72-78. , COI: 1:CAS:528:DC%2BC2cXitVWkt7vM
  • Maron, D., Ames, B.N., Revised methods for the Salmonella mutagenicity test (1983) Mutat Res, 113, pp. 173-215. , COI: 1:CAS:528:DyaL3sXhvFarsb4%3D
  • Matsumoto, S.T., Mantovani, M.S., Malagutti, M.I.A., Dias, A.L., Fonseca, I.C., Marin-Morales, M.A., Genotoxicity and mutagenicity of water contaminated with tannery effluents, as evaluated by the micronucleus test and comet assay using the fish Oreochromis niloticus and chromosome aberrations in onion root-tips (2006) Genet Mol, 29, pp. 148-158. , COI: 1:CAS:528:DC%2BD28XjvFSqtb4%3D
  • Mortelmans, K., Zeiger, E., The Ames Salmonella/microsome mutagenicity assay (2000) Mutat Res, 455, pp. 29-60. , COI: 1:CAS:528:DC%2BD3cXoslGhtr0%3D
  • (2014) New York. <> (Accessed, 12, p. 04. ,
  • OECD, (2006) OECD guideline for the testing of chemicals. Proposal for updating guideline 208. Terrestrial plant test: 208: seedling emergence and seedling growth test, , OECD Publications Service, Paris
  • Peruzzo, P.J., Porta, A.A., Ronco, A.E., Levels of glyphosate in surface waters, sediments and soils associated with direct sowing soybean cultivation in north pampasic region of Argentina (2008) Environ Pollut, 156, pp. 61-66. , COI: 1:CAS:528:DC%2BD1cXhtV2jsLbI
  • Peterson, H.G., Boutin, C., Martin, P.A., Freemark, K.E., Ruecker, N.J., Moody, M.J., Aquatic phyto-toxicity of 23 pesticides applied at expected environmental concentrations (1994) Aquat Toxicol, 28 (3), pp. 275-292. , COI: 1:CAS:528:DyaK2cXlvFyjuro%3D
  • PRVD-02, (2010) Imazethapyr, , Pest Management Regulatory Agency Health Canada Editors, Otawa: 118 p
  • Radetski, C.M., Cotelle, S., Férard, J.F., Classical and biochemical endpoints in the evaluation of phytotoxic effects caused by the herbicide trichloroacetate (2000) Environ Exp Bot, 44 (3), pp. 221-229. , COI: 1:CAS:528:DC%2BD3cXosFOqsLo%3D
  • Ramezani, M.K., Oliver, D., Kookana, R.S., Lao, W., Gill, G., Preston, C., Faster degradation of herbicidally-active enantiomer of imidazzolinones in soils (2010) Chemosphere, 79, pp. 1040-1045. , COI: 1:CAS:528:DC%2BC3cXlsFCjsrk%3D
  • Rank, J., Nielsen, M.H., Allium cepa anaphase-telophase root tip chromosome aberration assay on N-methyl-N-nitrosourea, maleic hydrazide, sodium azide, and ethyl methanesulfonate (1997) Mutat Res, 390, pp. 121-127. , COI: 1:CAS:528:DyaK2sXit1egsbg%3D
  • Romero, D.M., Ríos de Molina, M.C., Juárez, A.B., Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri (2011) Ecotoxicol Environ Saf, 74, pp. 741-747. , COI: 1:CAS:528:DC%2BC3MXlvVeqtbY%3D
  • Roshon, R.D., McCann, J.H., Thompson, D.G., Stephenson, G.R., Effects of seven forestry management herbicides on Myriophyllum sibiricum, as compared with other nontarget aquatic organisms (1999) Can J For Res, 29 (7), pp. 1158-1169
  • Shaner, D.L., Anderson, P.C., Stidham, M.A., Imidazolinones. Potent inhibitors of acetohydoxyacid synthase (1984) Plant Physiol, 76, pp. 545-546. , COI: 1:CAS:528:DyaL2MXisFSg
  • Siemering, G.S., Hayworth, J.D., Greenfield, B.K., Assesment of potential aquatic herbicide impacts to California aquatic ecosystems (2008) Arch Environ Contam Toxicol, 55, pp. 415-431. , COI: 1:CAS:528:DC%2BD1cXhtFaitr7O
  • Silveira Moraes, B., Clasen, B., Loro, V.L., Pretto, A., Toni, C., de Avila, L.A., Marchesan, E., Boschmann Reimche, G., Toxicological responses of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic (2011) Ecotoxicol Environ Saf, 74, pp. 328-335
  • Sobrero, C., Ronco, A., Ensayo de toxicidad aguda con semillas de L. sativa (2004) Ensayos toxicológicos y métodos de evaluación de calidad de aguas: estandarización, intercalibración, pp. 71-79. , Castillo G, (ed), Resultados y Aplicaciones, México
  • Srivastava, K., Mishra, K.K., Cytogenetic effects of commercially formulated atrazine on the somatic cells of Allium cepa and Vicia faba (2009) Pestic Biochem Physiol, 93, pp. 8-12. , COI: 1:CAS:528:DC%2BD1MXksVamsg%3D%3D
  • Tan, S., Evans, R.R., Dahmer, M.L., Singh, B.K., Shaner, D.L., Imidazolinone-tolerant crops: history, current status and future (2005) Pest Manag Sci, 61, pp. 246-257. , COI: 1:CAS:528:DC%2BD2MXitFOgurg%3D
  • Tsui, M.T., Chu, L.M., Aquatic toxicity of glyphosate-based formulations: comparison between different organisms and the effects of environmental factors (2003) Chemosphere, 52, pp. 1189-1197. , COI: 1:CAS:528:DC%2BD3sXkvVCitLY%3D
  • EPA, U.S., Seed germination/root elongation toxicity test (1996) Ecological effects test guidelines. Office of Prevention, Pesticides, and Toxic Substances, Series, p. 850. ,, Washington: D.C
  • (2002) EPA (United States Environmental Protection Agency), ,, Selenastrum capricornutum growth test. In: Short-term method for estimating the chronic toxicity of effluents and receiving water to freshwater organisms
  • Vendrell, E., de Barreda, G., Ferraz, D., Sabater, C., Carrasco, J.M., Effect of glyphosate on growth of four freshwater species of phytoplankton: a microplate bioassay (2009) Bull Environ Contam Toxicol, 82, pp. 538-542. , COI: 1:CAS:528:DC%2BD1MXjt1Gjsrc%3D
  • Wang, W., Freemark, K., The use of plants for environmental monitoring and assessment (1995) Ecotoxicol Environ Saf, 30, pp. 289-301. , COI: 1:CAS:528:DyaK2MXlt1Gmurk%3D
  • WHO, (2005) The WHO recommended classification of pesticides by hazard and guideline to classification, , Switzerland, Geneva
  • Young, B.J., Riera, N.I., Beily, M.E., Bres, P.A., Crespo, D.C., Ronco, A.E., Toxicity of the effluent from an anaerobic bioreactor treating cereal residues on Lactuca sativa (2012) Ecotoxicol Environ Saf, 76, pp. 182-186. , COI: 1:CAS:528:DC%2BC3MXhsFegtbbP
  • Zhang, C., Xu, J., Liu, X., Dong, F., Kong, Z., Sheng, Y., Zheng, Y., Impact of imazethapyr on the microbial community structure in agricultural soils (2010) Chemosphere, 81, pp. 800-806. , COI: 1:CAS:528:DC%2BC3cXht1Cmu7vE


---------- APA ----------
Magdaleno, A., Peralta Gavensky, M., Fassiano, A.V., Ríos de Molina, M.C., Santos, M., March, H., Moretton, J.,..., Juárez, Á.B. (2015) . Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays. Environmental Science and Pollution Research, 22(23), 19194-19202.
---------- CHICAGO ----------
Magdaleno, A., Peralta Gavensky, M., Fassiano, A.V., Ríos de Molina, M.C., Santos, M., March, H., et al. "Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays" . Environmental Science and Pollution Research 22, no. 23 (2015) : 19194-19202.
---------- MLA ----------
Magdaleno, A., Peralta Gavensky, M., Fassiano, A.V., Ríos de Molina, M.C., Santos, M., March, H., et al. "Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays" . Environmental Science and Pollution Research, vol. 22, no. 23, 2015, pp. 19194-19202.
---------- VANCOUVER ----------
Magdaleno, A., Peralta Gavensky, M., Fassiano, A.V., Ríos de Molina, M.C., Santos, M., March, H., et al. Phytotoxicity and genotoxicity assessment of imazethapyr herbicide using a battery of bioassays. Environ. Sci. Pollut. Res. 2015;22(23):19194-19202.