Artículo

Pritsch, K.; Courty, P.E.; Churin, J.-L.; Cloutier-Hurteau, B.; Ali, M.A.; Damon, C.; Duchemin, M.; Egli, S.; Ernst, J.; Fraissinet-Tachet, L.; Kuhar, F.; Legname, E.; Marmeisse, R.; Müller, A.; Nikolova, P.; Peter, M.; Plassard, C.; Richard, F. (...) Garbaye, J. "Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae" (2011) Mycorrhiza. 21(7):589-600
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Abstract:

The aim of a joint effort by different research teams was to provide an improved procedure for enzyme activity profiling of field-sampled ectomycorrhizae, including recommendations on the best conditions and maximum duration for storage of ectomycorrhizal samples. A more simplified and efficient protocol compared to formerly published procedures was achieved by using manufactured 96-filter plates in combination with a vacuum manifold and by optimizing incubation times. Major improvements were achieved by performing the series of eight enzyme assays with a single series of root samples instead of two series, reducing the time needed for sample preparation, minimizing error-prone steps such as pipetting and morphotyping, and facilitating subsequent DNA analyses due to the reduced sequencing effort. The best preservation of samples proved to be storage in soil at 4-6°C in the form of undisturbed soil cores containing roots. Enzyme activities were maintained for up to 4 weeks under these conditions. Short-term storage of washed roots and ectomycorrhizal tips overnight in water did not cause substantial changes in enzyme activity profiles. No optimal means for longer-term storage by freezing at -20°C or storage in 100% ethanol were recommended. © 2011 Springer-Verlag.

Registro:

Documento: Artículo
Título:Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae
Autor:Pritsch, K.; Courty, P.E.; Churin, J.-L.; Cloutier-Hurteau, B.; Ali, M.A.; Damon, C.; Duchemin, M.; Egli, S.; Ernst, J.; Fraissinet-Tachet, L.; Kuhar, F.; Legname, E.; Marmeisse, R.; Müller, A.; Nikolova, P.; Peter, M.; Plassard, C.; Richard, F.; Schloter, M.; Selosse, M.-A.; Franc, A.; Garbaye, J.
Filiación:Institute of Soil Ecology, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany
Biodiversidad y Biología Experimental, CABA, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
UMR 1136, Interactions Arbres Micro-organismes, Centre INRA de Nancy, 54280 Champenoux, France
Botanical Institute, University of Basel, 4056 Basel, Switzerland
Swiss Federal Research Institute WSL, 8903 Birmensdorf, Switzerland
UMR CNRS 5557 d'Ecologie Microbienne, Université de Lyon, Université Lyon 1, 69622 Villeurbanne Cedex, France
UMR 1222, Ecologie Fonctionelle et Biogéochimie des Sols, INRA/IRD/SupAgro, 34060 Montpellier Cedex 01, France
UMR 5175, Centre d'Ecologie Fonctionnelle et Evolutive, 34293 Montpellier 5, France
UMR Biodivers Genes and Communautes, INRA Pierroton, 33612 Cestas, France
Institute of Soil Ecology, Terrestrial Ecogenetics, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany
Institute of Soil Ecology, Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany
Palabras clave:Ectomycorrhizae; Enzyme activity profiling; Method optimization; Sample storage; enzyme; bioassay; ectomycorrhiza; enzyme activity; error analysis; ethanol; filter; freezing; genetic analysis; morphotype; optimization; preservation; research work; article; cold; enzymology; methodology; microbiological examination; microbiology; mycology; mycorrhiza; plant root; preservation; time; Cold Temperature; Enzymes; Microbiological Techniques; Mycology; Mycorrhizae; Plant Roots; Preservation, Biological; Time Factors
Año:2011
Volumen:21
Número:7
Página de inicio:589
Página de fin:600
DOI: http://dx.doi.org/10.1007/s00572-011-0364-4
Título revista:Mycorrhiza
Título revista abreviado:Mycorrhiza
ISSN:09406360
CODEN:MCORE
CAS:Enzymes
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09406360_v21_n7_p589_Pritsch

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Citas:

---------- APA ----------
Pritsch, K., Courty, P.E., Churin, J.-L., Cloutier-Hurteau, B., Ali, M.A., Damon, C., Duchemin, M.,..., Garbaye, J. (2011) . Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae. Mycorrhiza, 21(7), 589-600.
http://dx.doi.org/10.1007/s00572-011-0364-4
---------- CHICAGO ----------
Pritsch, K., Courty, P.E., Churin, J.-L., Cloutier-Hurteau, B., Ali, M.A., Damon, C., et al. "Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae" . Mycorrhiza 21, no. 7 (2011) : 589-600.
http://dx.doi.org/10.1007/s00572-011-0364-4
---------- MLA ----------
Pritsch, K., Courty, P.E., Churin, J.-L., Cloutier-Hurteau, B., Ali, M.A., Damon, C., et al. "Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae" . Mycorrhiza, vol. 21, no. 7, 2011, pp. 589-600.
http://dx.doi.org/10.1007/s00572-011-0364-4
---------- VANCOUVER ----------
Pritsch, K., Courty, P.E., Churin, J.-L., Cloutier-Hurteau, B., Ali, M.A., Damon, C., et al. Optimized assay and storage conditions for enzyme activity profiling of ectomycorrhizae. Mycorrhiza. 2011;21(7):589-600.
http://dx.doi.org/10.1007/s00572-011-0364-4