Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

We analyzed the ability of the bone marrow (BM) stromal cells to achieve confluence and their proliferative capacity in BM primary cultures from 30 untreated lung cancer patients (LCP), 27 breast cancer patients (BCP), and 30 normal controls (NC) when these confluent cells were induced to proliferate following four continuous subcultures. Moreover, we evaluated the production of interleukin-1 beta (IL-1β), transforming growth factor beta 1 (TGF-β1), fibronectin, and prostaglandin E2 (PGE2) by pure fibroblasts (fourth passage). A fibroblast colony-forming units (CFU-F) assay was used to investigate the proliferative and confluence capacity. Levels of IL-1β, TGF-β1, and fibronectin in conditioned mediums (CM) of fibroblast cultures were measured by enzyme-linked immunosorbent assay (ELISA) kit and PGE2 by radioimmunoassay (RIA) kit. Confluence was achieved in the 60% of LCP and 78% of BCP primary cultures compared with 100% of NC, and only fibroblasts from seven LCP and six BCP cultures had the capacity to proliferate following four subcultures. Levels of IL-1β were below 10 pg/ml in both patient groups, while NC had a mean value of 5882.57±221.61 pg/ml. Levels of TGF-β1 in BCP were lower than NC values (P<0.05). LCP and BCP had significantly decreased levels of fibronectin when compared to NC values (P<0.05 and P<0.01, respectively). Levels of PGE2 in LCP were higher compared to NC (P<0.01). In conclusion, BM fibroblasts from LCP and BCP presented a defective proliferative and confluence capacity, and this deficiency may be associated with the alteration of IL-1β, TGF-β1, fibronectin, and PGE2 production.

Registro:

Documento: Artículo
Título:Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients
Autor:Honegger, A.E.; Hofer, E.L.; Barañao, R.I.; Mackinlay, T.A.; Mackinlay, D.A.; Bullorsky, E.O.; Bordenave, R.H.; Chasseing, N.A.
Filiación:Inst. Biologia y Med. Experimental, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Department of Thorax, Británico Hospital, Buenos Aires, Argentina
Dept. of Hematol./Bone Marrow Trans., Británico Hospital, Buenos Aires, Argentina
Department of Oncology, I. Iriarte Hospital, Buenos Aires, Argentina
Palabras clave:Cancer; Fibroblasts; Soluble factors; fibronectin; interleukin 1beta; prostaglandin E2; transforming growth factor beta1; article; biosynthesis; bone marrow culture; breast cancer; cancer patient; cell assay; cell function; cell level; cell proliferation; colony forming unit; continuous culture; controlled study; culture medium; enzyme linked immunosorbent assay; fibroblast; human; human cell; lung cancer; major clinical study; priority journal; radioimmunoassay; stroma cell; Bone Marrow Cells; Breast Neoplasms; Carcinoma, Squamous Cell; Cell Division; Cells, Cultured; Culture Media, Conditioned; Dinoprostone; Female; Fibroblasts; Fibronectins; Humans; Interleukin-1; Lung Neoplasms; Transforming Growth Factor beta
Año:2002
Volumen:81
Número:2
Página de inicio:80
Página de fin:85
DOI: http://dx.doi.org/10.1007/s00277-001-0410-y
Título revista:Annals of Hematology
Título revista abreviado:Ann. Hematol.
ISSN:09395555
CODEN:ANHEE
CAS:fibronectin, 86088-83-7; prostaglandin E2, 363-24-6; Culture Media, Conditioned; Dinoprostone, 363-24-6; Fibronectins; Interleukin-1; Transforming Growth Factor beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395555_v81_n2_p80_Honegger

Referencias:

  • Bentley, S.A., Foidart, J.M., Some properties of marrow derived adherent cells in tissue culture (1980) Blood, 56, pp. 1006-1012
  • Bitterman, P.B., Rennard, S.I., Adelberg, S., Crystal, R.G., Role of fibronectin as a growth factor for fibroblasts (1983) J. Cell Biol., 97, pp. 1925-1932
  • Castro-Malaspina, H., Gay, R.E., Resnick, G., Kapoor, N., Meyers, P., Chiarieri, D., McKenzie, S., Moore, M.A.S., Characterization of human bone marrow fibroblast colony-forming cells (CFU-F) and their progeny (1980) Blood, 56, pp. 289-301
  • Chasseing, N.A., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Stemmelin, G.R., Rumi, L., Fibroblastic colony forming units and the levels of tumor necrosis factor and prostaglandin E2 in bone marrow cultures from patients with advanced lung carcinoma (1997) Cancer, 80, pp. 1914-1919
  • Chasseing, N.A., Trejo, Y.G., Bordenave, R.H., Bullorsky, E.O., Diaz, N.B., Rumi, L.S., Bone marrow fibroblastic progenitors in patients with advanced breast cancer (1997) Breast Cancer Res. Treat., 45, pp. 211-221
  • Colter, D.C., Class, R., DiGirolamo, C.M., Prockop, D.J., Rapid expansion of recycling stem cells in cultures of plastic-adherent cells from human bone marrow (2000) Proc. Natl. Acad. Sci. U. S. A., 97, pp. 3213-3218
  • Coutinho, L.H., Geary, C.G., Chang, J., Harrison, C., Testa, N.G., Functional studies of bone marrow haemopoietic and stromal cells in the myelodysplastic syndrome (MDS) (1990) Br. J. Haematol., 75, pp. 16-25
  • Eaves, A.C., Eaves, C.J., Maintenance and proliferation control of primitive hemopoietic progenitors in long-term cultures of marrow cells (1988) Blood Cells, 14, pp. 355-368
  • Freundlich, B., Bomalaski, J.S., Neilson, E., Jimenez, S.A., Regulation of fibroblast proliferation and collagen synthesis by cytokines (1986) Immunol. Today, 7, pp. 303-307
  • Friedenstein, A.J., Gorskaja, J.F., Kulagina, N.N., Fibroblast precursors in normal and irradiated mouse hematopoietic organs (1976) Exp. Hematol., 4, pp. 267-274
  • Gómez-Lechon, M.J., Castell, J.V., Enzyme-linked immunoabsorbent assay to quantity fibronectin (1985) Anal. Biochem., 145, pp. 1-6
  • Gospodarowicz, D., Greenburg, G., Birdwell, C.R., Determination of cellular shape by the extracellular matrix and its correlation with the control of cellular growth (1978) Cancer Res., 38, pp. 4155-4171
  • Hassan, H.T., Hanauske, A.R., Lux, E., Kleine, H.D., Freund, M., A new fibroblast growth stimulating activity from megakaryoblastic leukaemia cell line ELF-153: In vitro and in vivo findings (1995) Int. J. Exp. Pathol., 76, pp. 361-367
  • Hirata, J., Katsuno, M., Kaneko, S., Clinical significance of human bone marrow stromal cell colonies in acute leukemias (1986) Leuk. Res., 12, pp. 1441-1445
  • Hori, T., Yamanaka, Y., Hayakawa, M., Shibamoto, S., Tsujimoto, M., Oku, N., Ito, F., Prostaglandins antagonize fibroblast proliferation stimulated by tumor necrosis factor (1991) Biochem. Biophys. Res. Commun., 174, pp. 758-766
  • Kimura, A., Katoh, O., Hyodo, H., Kuramoto, A., Transforming growth factor beta regulates growth as well as collagen and fibronectin synthesis of human marrow fibroblasts (1989) Br. J. Haematol., 72, pp. 486-491
  • Kimura, A., Katoh, O., Kuramoto, A., Effects of platelet derived growth factor, epidermal growth factor and transforming growth factor-beta on the growth of human marrow fibroblasts (1988) Br. J. Haematol., 69, pp. 9-12
  • Ko, S.D., Page, R.C., Narayanan, A.S., Fibroblast heterogeneity and prostaglandin regulation of subpopulations (1977) Proc. Natl. Acad. Sci. U. S. A., 74, pp. 3429-3432
  • Korn, J.H., Halushka, P.V., LeRoy, E.C., Mononuclear cell modulation of connective tissue function. Suppression of fibroblast growth by stimulation of endogenous prostaglandin production (1980) J. Clin. Invest., 65, pp. 543-554
  • Kovacs, E.J., Fibrogenic cytokines: The role of immune mediators in the development of scar tissue (1991) Immunol. Today, 12, pp. 17-23
  • Kuznetsov, S.A., Friedenstein, A.J., Robey, P.G., Factors required for bone marrow stromal fibroblast colony formation in-vitro (1997) Br. J. Haematol., 97, pp. 561-570
  • Kuznetsov, S., Gehron, R.P., Species differences in growth requirements for bone marrow stromal fibroblast colony formation-in vitro (1996) Calcif. Tissue Int., 59, pp. 265-270
  • Lynn Allen-Hoffmann, B., Schlossert, S.J., Brondyk, W.H., Fahl, W.E., Fibronectin levels are enhanced in human fibroblasts overexpressing the c-sis protooncoge (1990) J. Biol. Chem., 265, pp. 5219-5224
  • Mayani, H., Guilbert, I.J., Janowska-Wieczorek, A., Biology of hematopoietic microenvironment (1992) Eur. J. Haematol., 49, pp. 225-233
  • Minguell, J.J., Conget, P., Erices, A., Biology and clinical utilization of mesenchymal progenitor cells (2000) Braz. J. Med. Biol. Res., 33, pp. 881-887
  • Nathan, C., Sporn, M., Cytokines in context (1991) J. Cell Biol., 113, pp. 981-986
  • Rougier, F., Dupuis, F., Denizot, Y., Human bone marrow fibroblasts - An overview of their characterization, proliferation and inflammatory mediator production (1996) Hematol. Cell Ther., 38, pp. 241-246
  • Thornton, S.C., Por, S.B., Walsh, B.J., Penny, R., Breit, S.N., Interaction of immune and connective tissue cells: I. The effect of lymphokines and monokines on fibroblast growth (1990) J. Leukoc. Biol., 47, pp. 312-320

Citas:

---------- APA ----------
Honegger, A.E., Hofer, E.L., Barañao, R.I., Mackinlay, T.A., Mackinlay, D.A., Bullorsky, E.O., Bordenave, R.H.,..., Chasseing, N.A. (2002) . Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients. Annals of Hematology, 81(2), 80-85.
http://dx.doi.org/10.1007/s00277-001-0410-y
---------- CHICAGO ----------
Honegger, A.E., Hofer, E.L., Barañao, R.I., Mackinlay, T.A., Mackinlay, D.A., Bullorsky, E.O., et al. "Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients" . Annals of Hematology 81, no. 2 (2002) : 80-85.
http://dx.doi.org/10.1007/s00277-001-0410-y
---------- MLA ----------
Honegger, A.E., Hofer, E.L., Barañao, R.I., Mackinlay, T.A., Mackinlay, D.A., Bullorsky, E.O., et al. "Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients" . Annals of Hematology, vol. 81, no. 2, 2002, pp. 80-85.
http://dx.doi.org/10.1007/s00277-001-0410-y
---------- VANCOUVER ----------
Honegger, A.E., Hofer, E.L., Barañao, R.I., Mackinlay, T.A., Mackinlay, D.A., Bullorsky, E.O., et al. Interleukin-1 beta, transforming growth factor beta 1, prostaglandin E2, and fibronectin levels in the conditioned mediums of bone marrow fibroblast cultures from lung and breast cancer patients. Ann. Hematol. 2002;81(2):80-85.
http://dx.doi.org/10.1007/s00277-001-0410-y