Artículo
Abstract:
Porphobilinogen-deaminase from Saccharomyces cerevisiae has been isolated and partially purified 80- and 230-fold in the absence or presence of phenylmethylsulphonyl fluoride, re-spectively. Some properties of the isolated enzyme were studied. Porphyrin formation was linear with time and protein concentration. Optimum pH was about 7.5-7.8. Molecular mass of the protein was 30,000 ± 3000 Dalton when the enzyme was purified in the presence of phenylmethyl-sulphonyl fluoride. A less active and unstable 20,000 Da molecular mass species was obtained when purification was performed in the absence of the protease inhibitor. Porphobilinogen-deaminase exhibited classical Michaelis-Menten kinetics. The apparent Km for uroporphyrinogen formation was 19 μM; Vmaxwas 3.6 nmol uroporphyrin/h and the Hill coefficient was n = 1. Also the action of several reagents on the activity was studied. Protective thiol agents had no effect. Heavy metals inhibited both porphyrin formation and porphobilinogen consumption, but known sulphydryl inactivating chemicals inhibit the former without modifying the latter. Ammonium ions had no effect on the activity while hydroxylamine completely inhibited both porphyrin formation and porphobilinogen consumption. © 1991, Verlag der Zeitschrift für Naturforschung. All rights reserved.
Registro:
| Documento: | Artículo |
| Título: | Studies on Porphobilinogen-Deaminase from Saccharomyces cerevisiae |
| Autor: | Correa Garcia, S.R.; Rossetti, M.V.; del Carmen Batlle, A.M. |
| Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias - CIPYP (CON1CET, F.C.E. y N., (UBA)-Ciudad Universitaria, Pabellon II, 2do piso, 1428 Buenos Aires, Argentina |
| Palabras clave: | Porphobilinogen; Porphobilinogen-Deaminase; Porphyrin Biosynthesis; Saccharomyces cerevisiae; Yeast; benzylsulfonyl fluoride; porphobilinogen deaminase; article; chemistry; enzymology; gel chromatography; isolation and purification; kinetics; metabolism; methodology; molecular weight; pH; Saccharomyces cerevisiae; Chromatography, Gel; Hydrogen-Ion Concentration; Hydroxymethylbilane Synthase; Kinetics; Molecular Weight; Phenylmethylsulfonyl Fluoride; Saccharomyces cerevisiae; Support, Non-U.S. Gov't |
| Año: | 1991 |
| Volumen: | 46 |
| Número: | 11-12 |
| Página de inicio: | 1017 |
| Página de fin: | 1023 |
| DOI: | http://dx.doi.org/10.1515/znc-1991-11-1215 |
| Título revista: | Zeitschrift fur Naturforschung - Section C Journal of Biosciences |
| Título revista abreviado: | Z. Naturforsch. Sect. C J. Biosci. |
| ISSN: | 09395075 |
| CAS: | benzylsulfonyl fluoride, 329-98-6; porphobilinogen deaminase, 9036-47-9, 9074-91-3; Hydroxymethylbilane Synthase, EC 4.3.1.8; Phenylmethylsulfonyl Fluoride, 329-98-6 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v46_n11-12_p1017_CorreaGarcia |
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Citas:
---------- APA ----------
Correa Garcia, S.R., Rossetti, M.V. & del Carmen Batlle, A.M. (1991) . Studies on Porphobilinogen-Deaminase from Saccharomyces cerevisiae. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 46(11-12), 1017-1023.http://dx.doi.org/10.1515/znc-1991-11-1215
---------- CHICAGO ----------
Correa Garcia, S.R., Rossetti, M.V., del Carmen Batlle, A.M. "Studies on Porphobilinogen-Deaminase from Saccharomyces cerevisiae" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences 46, no. 11-12 (1991) : 1017-1023.http://dx.doi.org/10.1515/znc-1991-11-1215
---------- MLA ----------
Correa Garcia, S.R., Rossetti, M.V., del Carmen Batlle, A.M. "Studies on Porphobilinogen-Deaminase from Saccharomyces cerevisiae" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 46, no. 11-12, 1991, pp. 1017-1023.http://dx.doi.org/10.1515/znc-1991-11-1215
---------- VANCOUVER ----------
Correa Garcia, S.R., Rossetti, M.V., del Carmen Batlle, A.M. Studies on Porphobilinogen-Deaminase from Saccharomyces cerevisiae. Z. Naturforsch. Sect. C J. Biosci. 1991;46(11-12):1017-1023.http://dx.doi.org/10.1515/znc-1991-11-1215
