Abstract:
A highly efficient method employing NaSCN as a chaotropic agent was used to dissociate the membrane-bound porphobilinogen-deaminase. The same sequence of steps was applied for purifying both soluble and membrane dissociated porphobilinogen-deaminase. The chromatographic behaviour of both proteins was quite similar. Euglena gracilis deaminase appears to exist in an equilibrium mixture of two active species of relative molecular masses of 40000 and 20000. © 1989, Verlag der Zeitschrift. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis |
Autor: | Rossetti, M.V.; Juknat, A.A.; del Batlle, A.M.C. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP, (FCEN, UBA, CONICET), Ciudad Universitaria, Pabellon II, 2do. Piso, 1428 Buenos Aires, Argentina
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Palabras clave: | Euglena gracilis; Particulate; Porphobilinogen-Deaminase; Porphyrins; Soluble |
Año: | 1989
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Volumen: | 44
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Número: | 7-8
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Página de inicio: | 578
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Página de fin: | 580
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DOI: |
http://dx.doi.org/10.1515/znc-1989-7-807 |
Título revista: | Zeitschrift fur Naturforschung - Section C Journal of Biosciences
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Título revista abreviado: | Z. Naturforsch. Sect. C J. Biosci.
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ISSN: | 09395075
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v44_n7-8_p578_Rossetti |
Referencias:
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- Kotler, M.L., Fumagalli, S.A., Juknat, A.A., del Battle, A.M.C., (1987) Comp. Biochem. Physiol, 87 B (3), p. 601
- Smythie, E., Williams, D.C., (1988) Biochem. J, 251, p. 237
- Rossetti, M.V., Lombardo, M.L., de Geralnik, A.A.J., Araujo, L.S., del Battle, A.M.C., (1986) Comp. Biochem. Physiol, 85 B (2), p. 451
- Juknat, A.A., Dörnemann, D., Senger, H., (1989) Z. Naturforsch, 44 c, pp. 81-94
Citas:
---------- APA ----------
Rossetti, M.V., Juknat, A.A. & del Batlle, A.M.C.
(1989)
. Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 44(7-8), 578-580.
http://dx.doi.org/10.1515/znc-1989-7-807---------- CHICAGO ----------
Rossetti, M.V., Juknat, A.A., del Batlle, A.M.C.
"Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis"
. Zeitschrift fur Naturforschung - Section C Journal of Biosciences 44, no. 7-8
(1989) : 578-580.
http://dx.doi.org/10.1515/znc-1989-7-807---------- MLA ----------
Rossetti, M.V., Juknat, A.A., del Batlle, A.M.C.
"Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis"
. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 44, no. 7-8, 1989, pp. 578-580.
http://dx.doi.org/10.1515/znc-1989-7-807---------- VANCOUVER ----------
Rossetti, M.V., Juknat, A.A., del Batlle, A.M.C. Soluble and Particulate Porphobilinogen-Deaminase from Dark-Grown Euglena gracilis. Z. Naturforsch. Sect. C J. Biosci. 1989;44(7-8):578-580.
http://dx.doi.org/10.1515/znc-1989-7-807