Abstract:
Lysed cells as well as different fractions of isolated, disrupted chloroplasts from light grown Euglena gracilis were tested for their capability of porphyrin biosynthesis. It is shown that the formation of the soluble porphobilinogenase (PBG-ase) fraction is inhibited by cycloheximide indicating its biosyntheses on 80S ribosomes, whereas the formation of the membrane bound chloroplast PBG-ase is chloramphenicol sensitive. Different pH-optima are demonstrated for the two enzymes. From the presented results it is deduced that PBG-ase from chloroplasts is different from those isolated from dark grown cells. © 1989, Verlag der Zeitschrift für Naturforschung. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z. |
Autor: | Juknat, A.A.; Dörnemann, D.; Senger, H. |
Filiación: | Fachbereich Biologie/Botanik, Philipps-Universität Marburg, D-3550 Marburg, Germany Centro de Investigationes sobre Porfirinas y Porfirias — (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Cuidad Universitaria, Pabellon II, 2°Piso, 1428 Buenos Aires, Argentina
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Palabras clave: | Euglena Chloroplasts; Euglena gracilis Z; Porphobilinogenase; Porphyrin Biosynthesis; Porphyrinogens |
Año: | 1989
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Volumen: | 44
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Número: | 1-2
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Página de inicio: | 85
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Página de fin: | 96
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DOI: |
http://dx.doi.org/10.1515/znc-1989-1-216 |
Título revista: | Zeitschrift fur Naturforschung - Section C Journal of Biosciences
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Título revista abreviado: | Z. Naturforsch. Sect. C J. Biosci.
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ISSN: | 09395075
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v44_n1-2_p85_Juknat |
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Citas:
---------- APA ----------
Juknat, A.A., Dörnemann, D. & Senger, H.
(1989)
. Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 44(1-2), 85-96.
http://dx.doi.org/10.1515/znc-1989-1-216---------- CHICAGO ----------
Juknat, A.A., Dörnemann, D., Senger, H.
"Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z."
Zeitschrift fur Naturforschung - Section C Journal of Biosciences 44, no. 1-2
(1989) : 85-96.
http://dx.doi.org/10.1515/znc-1989-1-216---------- MLA ----------
Juknat, A.A., Dörnemann, D., Senger, H.
"Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z."
Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 44, no. 1-2, 1989, pp. 85-96.
http://dx.doi.org/10.1515/znc-1989-1-216---------- VANCOUVER ----------
Juknat, A.A., Dörnemann, D., Senger, H. Different Porphobilinogenases in Cytoplasm and Isolated Chloroplasts from Light-Grown Euglena gracilis Z. Z. Naturforsch. Sect. C J. Biosci. 1989;44(1-2):85-96.
http://dx.doi.org/10.1515/znc-1989-1-216