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Abstract:

A low molecular weight, heat-stable factor has been purified from Euglena gracilis supernatant fraction by employing gel filtration, cation and anion exchange and paper chromatography. This endogenous compound stimulates porphobilinogenase (PBG-ase) (EC 4.3.1.8) activity, an enzyme of the porphyrin biosynthetic pathway. 1CT7 M folic acid and ICI-4 M 6-biopterin produced a significant activation, equivalent to 2–4 units of the purified factor. Elution patterns from the columns and fluorescence and UV absorption peaks suggest that this compound is a pteridine. This conclusion is further supported by the fact that both, folic acid and 6-biopterin can replace the action of the isolated factor on PBG-ase. The mechanism of stimulation is discussed. © 1988, Walter de Gruyter. All rights reserved.

Registro:

Documento: Artículo
Título:Biosynthesis of porphyrinogens in etiolated euglena gracilis z. i. isolation and purification of an endogenous factor stimulating the formation of porphyrinogens
Autor:Juknat, A.A.; Dörnemann, D.; Senger, H.
Filiación:Centro de Investigaciones sobre Porfirinas y Porfirias – (CIPYP), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 2° Piso, 1428 Buenos Aires, Argentina
Fachbereich Biologie/Botanik, Philipps-Universität Marburg, Lahnberge, D-3550 Marburg, Germany
Palabras clave:Euglena gracilis Z; Porphobilinogenase; Porphyrin Biosynthesis; Porphyrinogens; Pteridines
Año:1988
Volumen:43
Número:5-6
Página de inicio:351
Página de fin:356
DOI: http://dx.doi.org/10.1515/znc-1988-5-605
Título revista:Zeitschrift fur Naturforschung - Section C Journal of Biosciences
Título revista abreviado:Z. Naturforsch. Sect. C J. Biosci.
ISSN:09395075
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09395075_v43_n5-6_p351_Juknat

Referencias:

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Citas:

---------- APA ----------
Juknat, A.A., Dörnemann, D. & Senger, H. (1988) . Biosynthesis of porphyrinogens in etiolated euglena gracilis z. i. isolation and purification of an endogenous factor stimulating the formation of porphyrinogens. Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 43(5-6), 351-356.
http://dx.doi.org/10.1515/znc-1988-5-605
---------- CHICAGO ----------
Juknat, A.A., Dörnemann, D., Senger, H. "Biosynthesis of porphyrinogens in etiolated euglena gracilis z. i. isolation and purification of an endogenous factor stimulating the formation of porphyrinogens" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences 43, no. 5-6 (1988) : 351-356.
http://dx.doi.org/10.1515/znc-1988-5-605
---------- MLA ----------
Juknat, A.A., Dörnemann, D., Senger, H. "Biosynthesis of porphyrinogens in etiolated euglena gracilis z. i. isolation and purification of an endogenous factor stimulating the formation of porphyrinogens" . Zeitschrift fur Naturforschung - Section C Journal of Biosciences, vol. 43, no. 5-6, 1988, pp. 351-356.
http://dx.doi.org/10.1515/znc-1988-5-605
---------- VANCOUVER ----------
Juknat, A.A., Dörnemann, D., Senger, H. Biosynthesis of porphyrinogens in etiolated euglena gracilis z. i. isolation and purification of an endogenous factor stimulating the formation of porphyrinogens. Z. Naturforsch. Sect. C J. Biosci. 1988;43(5-6):351-356.
http://dx.doi.org/10.1515/znc-1988-5-605