Artículo

Inbar, E.; Canepa, G.E.; Carrillo, C.; Glaser, F.; Grotemeyer, M.S.; Rentsch, D.; Zilberstein, D.; Pereira, C.A. "Lysine transporters in human trypanosomatid pathogens" (2012) Amino Acids. 42(1):347-360
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Abstract:

In previous studies we characterized arginine transporter genes from Trypanosoma cruzi and Leishmania donovani, the etiological agents of chagas disease and kala azar, respectively, both fatal diseases in humans. Unlike arginine transporters in higher eukaryotes that transport also lysine, these parasite transporters translocate only arginine. This phenomenon prompted us to identify and characterize parasite lysine transporters. Here we demonstrate that LdAAP7 and TcAAP7 encode lysine-specific permeases in L. donovani and T. cruzi, respectively. These two lysine permeases are both members of the large amino acid/auxin permease family and share certain biochemical properties, such as specificity and Km. However, we evidence that LdAAP7 and TcAAP7 differ in their regulation and localization, such differences are likely a reflection of the dissimilar L. donovani and T. cruzi life cycles. Failed attempts to delete both alleles of LdAAP7 support the premise that this is an essential gene that encodes the only lysine permeases expressed in L. donovani promastigotes and T. cruzi epimastigotes, respectively. © Springer-Verlag 2010.

Registro:

Documento: Artículo
Título:Lysine transporters in human trypanosomatid pathogens
Autor:Inbar, E.; Canepa, G.E.; Carrillo, C.; Glaser, F.; Grotemeyer, M.S.; Rentsch, D.; Zilberstein, D.; Pereira, C.A.
Filiación:Faculty of Biology, Technion-Israel Institute of Technology, 32000 Haifa, Israel
Departamento de Sustancias Vasoactivas, Laboratorio de Biología Molecular de Trypanosoma Cruzi (LBMTC), Instituto de Investigaciones Médicas Alfredo Lanari, Buenos Aires, Argentina
Fundación Instituto Leloir, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires and CONICET, Buenos Aires, Argentina
Institute of Plant Sciences, University of Bern, 3013 Bern, Switzerland
Palabras clave:Amino acid transport; Leishmania; Lysine transport; Trypanosoma; alanine; amino acid transporter; arginine; asparagine; aspartic acid; auxin; cysteine; glycine; histidine; Leishmania donovani AAP7; lysine; lysine transporter; methionine; permease; proline; serine; threonine; Trypanosoma cruzi AAP7; unclassified drug; conference paper; enzyme kinetics; enzyme regulation; enzyme specificity; epimastigote; human; immunofluorescence test; Leishmania donovani; life cycle; nucleotide sequence; open reading frame; parasite survival; phylogeny; priority journal; promastigote; protein expression; protein localization; Saccharomyces cerevisiae; sequence analysis; Trypanosoma; Trypanosoma cruzi; trypanosomatid infection; Eukaryota; Leishmania donovani; Naso hexacanthus; Trypanosoma; Trypanosoma cruzi; Trypanosomatidae
Año:2012
Volumen:42
Número:1
Página de inicio:347
Página de fin:360
DOI: http://dx.doi.org/10.1007/s00726-010-0812-z
Título revista:Amino Acids
Título revista abreviado:Amino Acids
ISSN:09394451
CODEN:AACIE
CAS:alanine, 56-41-7, 6898-94-8; arginine, 1119-34-2, 15595-35-4, 7004-12-8, 74-79-3; asparagine, 70-47-3, 7006-34-0; aspartic acid, 56-84-8, 6899-03-2; cysteine, 4371-52-2, 52-89-1, 52-90-4; glycine, 56-40-6, 6000-43-7, 6000-44-8; histidine, 645-35-2, 7006-35-1, 71-00-1; lysine, 56-87-1, 6899-06-5, 70-54-2; methionine, 59-51-8, 63-68-3, 7005-18-7; proline, 147-85-3, 7005-20-1; serine, 56-45-1, 6898-95-9; threonine, 36676-50-3, 72-19-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09394451_v42_n1_p347_Inbar

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Citas:

---------- APA ----------
Inbar, E., Canepa, G.E., Carrillo, C., Glaser, F., Grotemeyer, M.S., Rentsch, D., Zilberstein, D.,..., Pereira, C.A. (2012) . Lysine transporters in human trypanosomatid pathogens. Amino Acids, 42(1), 347-360.
http://dx.doi.org/10.1007/s00726-010-0812-z
---------- CHICAGO ----------
Inbar, E., Canepa, G.E., Carrillo, C., Glaser, F., Grotemeyer, M.S., Rentsch, D., et al. "Lysine transporters in human trypanosomatid pathogens" . Amino Acids 42, no. 1 (2012) : 347-360.
http://dx.doi.org/10.1007/s00726-010-0812-z
---------- MLA ----------
Inbar, E., Canepa, G.E., Carrillo, C., Glaser, F., Grotemeyer, M.S., Rentsch, D., et al. "Lysine transporters in human trypanosomatid pathogens" . Amino Acids, vol. 42, no. 1, 2012, pp. 347-360.
http://dx.doi.org/10.1007/s00726-010-0812-z
---------- VANCOUVER ----------
Inbar, E., Canepa, G.E., Carrillo, C., Glaser, F., Grotemeyer, M.S., Rentsch, D., et al. Lysine transporters in human trypanosomatid pathogens. Amino Acids. 2012;42(1):347-360.
http://dx.doi.org/10.1007/s00726-010-0812-z