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Abstract:

Dactylogyridae is overwhelmingly the most abundant and diverse taxon among monogeneans in continental waters of South America. Their small body size requires considerable sampling effort and training for collecting and identifying the worms from the gills, skin, nasal cavities, and other microhabitats. Indeed, diagnostic characteristics as sclerites and male copulatory complex are generally less than 100-μm long and are essential for taxonomic description and identification of species. Here, a combination of simple and routine methods for three-dimensional morphological studies on hard structures is proposed for dactylogirids: SDS treatment for clarification of specimens and enzymatic digestion with proteinase K for freeing sclerotized structures, followed by laser confocal microscopy. This method is applicable to fresh or fixed specimens and does not require staining or dehydration. Indeed, stable autofluorescence emission is detectable at 500–530 nm for bars, anchors, and male copulatory complex when excited by argon laser. Advantages of this protocol over previous methodologies for taking laser confocal images are discussed. Open access software for image processing was used for three-dimensional reconstruction of sclerotized structures generating models and full 360° rotation videos. © 2017, Springer-Verlag GmbH Germany.

Registro:

Documento: Artículo
Título:Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)
Autor:Rossin, M.A.; De Francesco, P.N.; Timi, J.T.
Filiación:Laboratorio de Ictioparasitología, Instituto de Investigaciones Marinas y Costeras (IIMIyC), Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) Funes 3350, Mar del Plata, Argentina
Laboratorio de Neurofisiología, Instituto Multidisciplinario de Biología Celular (IMBICE), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)—Universidad Nacional de La Plata—Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), Calle 526 y Cno. Gral. Belgrano, La Plata, Argentina
Palabras clave:Autofluorescence; Laser confocal images; Monogenea; Proteinase K treatment; SDS treatment; Three-dimensional reconstruction; proteinase K; Article; autofluorescence; body size; Dactylogyridae; female; fluorescence microscopy; image processing; male; morphology; nonhuman; priority journal; structure analysis; taxonomy; three dimensional imaging; animal; Characiformes; classification; confocal microscopy; fish disease; gill; isolation and purification; lake; nose cavity; parasitology; river; South America; three dimensional imaging; trematode; trematodiasis; ultrastructure; veterinary; Animals; Characiformes; Fish Diseases; Gills; Image Processing, Computer-Assisted; Imaging, Three-Dimensional; Lakes; Male; Microscopy, Confocal; Nasal Cavity; Rivers; South America; Trematoda; Trematode Infections
Año:2017
Volumen:116
Número:10
Página de inicio:2813
Página de fin:2819
DOI: http://dx.doi.org/10.1007/s00436-017-5591-y
Título revista:Parasitology Research
Título revista abreviado:Parasitol. Res.
ISSN:09320113
CODEN:PARRE
CAS:proteinase K, 39450-01-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v116_n10_p2813_Rossin

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Citas:

---------- APA ----------
Rossin, M.A., De Francesco, P.N. & Timi, J.T. (2017) . Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea). Parasitology Research, 116(10), 2813-2819.
http://dx.doi.org/10.1007/s00436-017-5591-y
---------- CHICAGO ----------
Rossin, M.A., De Francesco, P.N., Timi, J.T. "Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)" . Parasitology Research 116, no. 10 (2017) : 2813-2819.
http://dx.doi.org/10.1007/s00436-017-5591-y
---------- MLA ----------
Rossin, M.A., De Francesco, P.N., Timi, J.T. "Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea)" . Parasitology Research, vol. 116, no. 10, 2017, pp. 2813-2819.
http://dx.doi.org/10.1007/s00436-017-5591-y
---------- VANCOUVER ----------
Rossin, M.A., De Francesco, P.N., Timi, J.T. Three-dimensional morphology of rigid structures as a tool for taxonomic studies of Dactylogyridae (Monogenea). Parasitol. Res. 2017;116(10):2813-2819.
http://dx.doi.org/10.1007/s00436-017-5591-y