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Abstract:

Dirofilariasis, a mosquito-borne disease of dogs caused by the nematode Dirofilaria immitis (Leidy; Spirurida: Onchocercidae), has now become a growing zoonotic concern. Based on direct microscopical observation, Aedes aegypti (L.) and Culex pipiens L. (Diptera: Culicidae) have been previously incriminated as potential vectors of D. immitis in urban temperate Argentina. In this study, an effort was made to provide evidence for this assumption by screening of mosquitoes for D. immitis infection using a polymerase chain reaction (PCR) assay. PCR primers were developed to specifically amplify the D. immitis-16S rRNA gene and to reliably detect 100th of the genomic equivalent (10 pg) of the infective third-stage larvae in mosquito pools of up to 30 individuals. Collection of mosquitoes was performed between September 2007 and April 2008 in premises known to be inhabited by D. immitis-infected dogs in Greater Buenos Aires. The final collection comprised 453 specimens belonging to 11 mosquito species of the genera Aedes, Culex, Ochlerotatus, and Psorophora. PCR assays were performed on 82 pools (n≤20) of heads and abdomens separately, as this allows differentiating infective and non-infective stages of the parasite, respectively. Identification of the non-infective stage of D. immitis in A. aegypti and C. pipiens provided additional strong support of transmission of the parasite by these species. To our knowledge, this was the first PCR screening for D. immitis-infected mosquitoes in South America. © Springer-Verlag 2010.

Registro:

Documento: Artículo
Título:PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina
Autor:Vezzani, D.; Mesplet, M.; Eiras, D.F.; Fontanarrosa, M.F.; Schnittger, L.
Filiación:Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón 2, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina
Instituto de Patobiología, CICVyA, INTA-Castelar, Buenos Aires, Argentina
Laboratorio DIAP (Diagnóstico en Animales Pequeños), Pueyrredón 1098 (B1828ADD), Banfield, Buenos Aires, Argentina
Departamento de Epizootiología Y Salud Pública, Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata, CC 296, (B1900AVW) La Plata, Argentina
Palabras clave:RNA 16S; Aedes aegypti; animal tissue; Argentina; arthropod larva; article; controlled study; Culex pipiens; Dirofilaria immitis; gene amplification; mosquito; nonhuman; nucleotide sequence; Ochlerotatus; parasite identification; polymerase chain reaction; priority journal; Psorophora; reliability; urban area; Aedes; Animals; Argentina; Culex; Dirofilaria immitis; DNA Primers; DNA, Helminth; Molecular Sequence Data; Parasitology; Polymerase Chain Reaction; RNA, Helminth; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Aedes aegypti; Canis familiaris; Culex pipiens; Culicidae; Diptera; Dirofilaria immitis; Onchocercidae; Psorophora; Spirurida
Año:2011
Volumen:108
Número:4
Página de inicio:985
Página de fin:989
DOI: http://dx.doi.org/10.1007/s00436-010-2142-1
Título revista:Parasitology Research
Título revista abreviado:Parasitol. Res.
ISSN:09320113
CODEN:PARRE
CAS:DNA Primers; DNA, Helminth; RNA, Helminth; RNA, Ribosomal, 16S
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v108_n4_p985_Vezzani

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Citas:

---------- APA ----------
Vezzani, D., Mesplet, M., Eiras, D.F., Fontanarrosa, M.F. & Schnittger, L. (2011) . PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina. Parasitology Research, 108(4), 985-989.
http://dx.doi.org/10.1007/s00436-010-2142-1
---------- CHICAGO ----------
Vezzani, D., Mesplet, M., Eiras, D.F., Fontanarrosa, M.F., Schnittger, L. "PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina" . Parasitology Research 108, no. 4 (2011) : 985-989.
http://dx.doi.org/10.1007/s00436-010-2142-1
---------- MLA ----------
Vezzani, D., Mesplet, M., Eiras, D.F., Fontanarrosa, M.F., Schnittger, L. "PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina" . Parasitology Research, vol. 108, no. 4, 2011, pp. 985-989.
http://dx.doi.org/10.1007/s00436-010-2142-1
---------- VANCOUVER ----------
Vezzani, D., Mesplet, M., Eiras, D.F., Fontanarrosa, M.F., Schnittger, L. PCR detection of Dirofilaria immitis in Aedes aegypti and Culex pipiens from urban temperate Argentina. Parasitol. Res. 2011;108(4):985-989.
http://dx.doi.org/10.1007/s00436-010-2142-1