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Abstract:

A low-stringency single-primer polymerase chain reaction (LSSP-PCR) typing procedure targeted to the intergenic regions of spliced-leader genes (SL) was designed to profile Trypanosoma cruzi I stocks from endemic regions of Colombia. Comparison between SL-LSSP-PCR profiles of parasite DNA from vector faeces and cultures isolated from those faeces showed more conservative signatures than profiles using LSSP-PCR targeted to the minicircle variable regions (kDNA). This was also observed by analysing 15 parasite clones from one stock as well as serial samples of a same stock after in vitro culturing or inoculation into mice. Thus, SL-LSSP-PCR appears more appropriate than kDNA-LSSP-PCR for reliable typing of major T. cruzi I groups from in vitro cultured stocks and triatomine faeces. SL-LSSP-PCR grouped 46 of 47 T. cruzi I Colombian stocks according to their geographical procedences in four clusters: Cluster Cas from Casanare Department, Cluster Mg from Northern Magdalena department, Cluster Mom from Momposina Depression in Southern Magdalena and finally Cluster NW from northwestern Colombia, including Sucre, Chocó, Córdoba and Antioquia departments. Sequence analysis identified punctual mutations among amplicons from each cluster. Within Cluster Mg, sequence polymorphism allowed association with different sylvatic vector species. Novel SL sequences and LSSP-PCR profiles are reported from T. cruzi I infecting Eratyrus cuspidatus, Panstrongylus geniculatus and Rhodnius pallescens vectors. © 2008 Springer-Verlag.

Registro:

Documento: Artículo
Título:Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes
Autor:Mejía-Jaramillo, A.M.; Arboleda-Sánchez, S.; Rodríguez, I.B.; Cura, C.; Salazar, A.; Del Mazo, J.; Triana-Chávez, O.; Schijman, A.G.
Filiación:Grupo de Chagas, Universidad de Antioquia, Medellin AA 1226, Colombia
Centro de Investigaciones Biológicas, CSIC, Madrid, Spain
Laboratorio de Biología Molecular de la Enfermedad de Chagas, INGEBI-CONICET, Buenos Aires, Argentina
Vuelta de Obligado 2490, Buenos Aires 1428, Argentina
Palabras clave:spacer DNA; animal husbandry; article; Colombia; controlled study; feces analysis; gene mutation; insect; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; sequence analysis; Trypanosoma cruzi; Animals; Cluster Analysis; Colombia; DNA, Intergenic; DNA, Protozoan; Genotype; Geography; Phylogeny; Polymerase Chain Reaction; Polymorphism, Genetic; RNA, Spliced Leader; Sequence Analysis, DNA; Triatominae; Trypanosoma cruzi; Eratyrus; Mus; Panstrongylus geniculatus; Rhodnius pallescens; Trypanosoma cruzi
Año:2009
Volumen:104
Número:2
Página de inicio:399
Página de fin:410
DOI: http://dx.doi.org/10.1007/s00436-008-1212-0
Título revista:Parasitology Research
Título revista abreviado:Parasitol. Res.
ISSN:09320113
CODEN:PARRE
CAS:DNA, Intergenic; DNA, Protozoan; RNA, Spliced Leader
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09320113_v104_n2_p399_MejiaJaramillo

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Citas:

---------- APA ----------
Mejía-Jaramillo, A.M., Arboleda-Sánchez, S., Rodríguez, I.B., Cura, C., Salazar, A., Del Mazo, J., Triana-Chávez, O.,..., Schijman, A.G. (2009) . Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes. Parasitology Research, 104(2), 399-410.
http://dx.doi.org/10.1007/s00436-008-1212-0
---------- CHICAGO ----------
Mejía-Jaramillo, A.M., Arboleda-Sánchez, S., Rodríguez, I.B., Cura, C., Salazar, A., Del Mazo, J., et al. "Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes" . Parasitology Research 104, no. 2 (2009) : 399-410.
http://dx.doi.org/10.1007/s00436-008-1212-0
---------- MLA ----------
Mejía-Jaramillo, A.M., Arboleda-Sánchez, S., Rodríguez, I.B., Cura, C., Salazar, A., Del Mazo, J., et al. "Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes" . Parasitology Research, vol. 104, no. 2, 2009, pp. 399-410.
http://dx.doi.org/10.1007/s00436-008-1212-0
---------- VANCOUVER ----------
Mejía-Jaramillo, A.M., Arboleda-Sánchez, S., Rodríguez, I.B., Cura, C., Salazar, A., Del Mazo, J., et al. Geographical clustering of Trypanosoma cruzi I groups from Colombia revealed by low-stringency single specific primer-PCR of the intergenic regions of spliced-leader genes. Parasitol. Res. 2009;104(2):399-410.
http://dx.doi.org/10.1007/s00436-008-1212-0