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Abstract:

This review aims to present different aspects related to cruzipain, one of the most important proteins of the etiological agent of Chagas disease that has been extensively studied in the last two decades, including all the particularities of the molecule as well as to highlight its participation in multiple relevant functions of the parasite to favour the cell invasion phenomena, to facilitate host tissues proteolytic degradation and to trigger the evasion mechanism from host immune response. Cruzipain has been related with parasite metabolism and identified as both an important candidate for vaccine development and for trypanocidal drug design. We have reported for the first time that this enzyme is a sulfated glycoprotein. Indeed, the sulfated oligosaccharides are main targets for immune responses and are involved in tissue damage in mice immunized in absence of infection contributing to get deeper into the knowledge of the molecule composition and helping to elucidate its role in the infection and/or pathogenesis of the disease. A whole view including all the aspects related to the major cysteine proteinase of Trypanosoma cruzi studied so far including recent advances as proteinase, antigen and glycoprotein will be discussed. © 2009 Bentham Science Publishers Ltd.

Registro:

Documento: Artículo
Título:Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review
Autor:Duschak, V.G.; Couto, A.S.
Filiación:Instituto Nacional de Parasitología Dr. Mario Fatala Chabén, ANLIS-Malbrán, Ministerio de Salud, Av. Paseo Colon 568, (1063) Buenos Aires, Argentina
CIHIDECAR(CONICET), Depto. de Química Orgánica, Universidad de Buenos Aires, Buenos Aires 1428, Argentina
Palabras clave:2,3,5,6 tetrafluorophenoxymethyl ketone; alpha 2 macroglobulin; antitrypanosomal agent; aryl 4 oxothiazolylhydrazone derivative; bisarylacylhydrazide derivative; chagasin; CpG oligodeoxynucleotide; cruzipain; cystatin; cysteine proteinase; cysteine proteinase inhibitor; dipeptidyl allyl sulphone derivative; dipeptidyl vinyl sulphone derivative; hydrolase inhibitor; k 11777; k 777; kininogen; n methyl pip phenylalanine homo phenylalanine vinyl sulfonylurea phenyl; peptide hydrolase inhibitor; proteinase inhibitor; recombinant cruzipain; recombinant enzyme; sialic acid; unclassified drug; virulence factor; cruzipain; cysteine proteinase; cysteine proteinase inhibitor; parasite antigen; protozoal vaccine; catalysis; cell invasion; Chagas disease; chemical composition; dog disease; drug structure; drug targeting; enzyme analysis; enzyme binding; enzyme degradation; enzyme mechanism; enzyme purification; enzyme structure; enzyme substrate; gene sequence; glycosylation; heart protection; host parasite interaction; immune response; mammal; nonhuman; phagocyte; protein analysis; protein domain; protein expression; protein polymorphism; protozoal infection; review; structure activity relation; structure analysis; target cell; tissue injury; Trypanosoma cruzi; vaccine production; animal; Chagas disease; chemistry; enzymology; human; immunology; metabolism; mouse; Trypanosoma cruzi; Animals; Antigens, Protozoan; Chagas Disease; Cysteine Endopeptidases; Cysteine Proteinase Inhibitors; Glycosylation; Humans; Mice; Protozoan Vaccines; Trypanosoma cruzi
Año:2009
Volumen:16
Número:24
Página de inicio:3174
Página de fin:3202
DOI: http://dx.doi.org/10.2174/092986709788802971
Título revista:Current Medicinal Chemistry
Título revista abreviado:Curr. Med. Chem.
ISSN:09298673
CODEN:CMCHE
CAS:alpha 2 macroglobulin, 95568-41-5; cystatin, 81989-95-9; cysteine proteinase, 37353-41-6; proteinase inhibitor, 37205-61-1; Antigens, Protozoan; Cysteine Endopeptidases, 3.4.22.-; Cysteine Proteinase Inhibitors; Protozoan Vaccines; cruzipain, 3.4.22.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09298673_v16_n24_p3174_Duschak

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Citas:

---------- APA ----------
Duschak, V.G. & Couto, A.S. (2009) . Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review. Current Medicinal Chemistry, 16(24), 3174-3202.
http://dx.doi.org/10.2174/092986709788802971
---------- CHICAGO ----------
Duschak, V.G., Couto, A.S. "Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review" . Current Medicinal Chemistry 16, no. 24 (2009) : 3174-3202.
http://dx.doi.org/10.2174/092986709788802971
---------- MLA ----------
Duschak, V.G., Couto, A.S. "Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review" . Current Medicinal Chemistry, vol. 16, no. 24, 2009, pp. 3174-3202.
http://dx.doi.org/10.2174/092986709788802971
---------- VANCOUVER ----------
Duschak, V.G., Couto, A.S. Cruzipain, the major cysteine protease of Trypanosoma cruzi: A sulfated glycoprotein antigen as relevant candidate for vaccine development and drug target. A review. Curr. Med. Chem. 2009;16(24):3174-3202.
http://dx.doi.org/10.2174/092986709788802971