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Abstract:

The Trypanosoma cruzi ribosomal P0 protein (TcP0) is part of the ribosomal stalk, which is an elongated lateral protuberance of the large ribosomal subunit involved in the translocation step of protein synthesis. The TcP0 C-terminal peptide is highly antigenic and a major target of the antibody response in patients with systemic lupus erythematosus and patients suffering chronic heart disease produced by Trypanosoma cruzi infection. The structural properties of TcP0 have been explored by circular dichroism, tryptophan fluorescence and limited proteolysis experiments. These studies were complemented by secondary structure consensus prediction analysis. The results suggest that the tertiary structure of TcP0 could be described as a compact, stable, trypsin-resistant, 200 residues long N-terminal domain belonging to the α/β class and a more flexible, degradable, helical, 123 residues long C-terminal domain which could be involved in the formation of an unusual hydrophobic zipper with the ribosomal P1/P2 proteins to form the P0/P1/P2 complex. © 2005 Bentham Science Publishers Ltd.

Registro:

Documento: Artículo
Título:Preliminary structural studies of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi, a part of the P0/P1/P2 complex
Autor:Ayub, M.J.; Barroso, J.A.; Levin, M.J.; Aguilar, C.F.
Filiación:Laboratorio de Biología Molecular Estructural, Facultad de Química, Bioquímica Y Farmacia, Universidad Nacional de San Luis, Ejército de los Andes 950, 5700 San Luis, Argentina
Inst. de Investigaciones en Ingeniería Genética Y Biología Molecular (INGEBI), Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Ribosomal P0 protein; Structural Studies; Trypanosoma cruzi; multiprotein complex; phosphoprotein; phosphoprotein P0; recombinant protein; ribosome protein; amino acid sequence; animal; article; chemistry; circular dichroism; genetics; hydrophobicity; metabolism; molecular genetics; protein binding; protein secondary structure; Trypanosoma cruzi; Amino Acid Sequence; Animals; Circular Dichroism; Hydrophobicity; Molecular Sequence Data; Multiprotein Complexes; Phosphoproteins; Protein Binding; Protein Structure, Secondary; Recombinant Proteins; Ribosomal Proteins; Trypanosoma cruzi; Trypanosoma cruzi
Año:2005
Volumen:12
Número:6
Página de inicio:521
Página de fin:525
DOI: http://dx.doi.org/10.2174/0929866054395879
Título revista:Protein and Peptide Letters
Título revista abreviado:Protein Pept. Lett.
ISSN:09298665
CODEN:PPELE
CAS:Multiprotein Complexes; phosphoprotein P0; Phosphoproteins; Recombinant Proteins; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09298665_v12_n6_p521_Ayub

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Citas:

---------- APA ----------
Ayub, M.J., Barroso, J.A., Levin, M.J. & Aguilar, C.F. (2005) . Preliminary structural studies of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi, a part of the P0/P1/P2 complex. Protein and Peptide Letters, 12(6), 521-525.
http://dx.doi.org/10.2174/0929866054395879
---------- CHICAGO ----------
Ayub, M.J., Barroso, J.A., Levin, M.J., Aguilar, C.F. "Preliminary structural studies of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi, a part of the P0/P1/P2 complex" . Protein and Peptide Letters 12, no. 6 (2005) : 521-525.
http://dx.doi.org/10.2174/0929866054395879
---------- MLA ----------
Ayub, M.J., Barroso, J.A., Levin, M.J., Aguilar, C.F. "Preliminary structural studies of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi, a part of the P0/P1/P2 complex" . Protein and Peptide Letters, vol. 12, no. 6, 2005, pp. 521-525.
http://dx.doi.org/10.2174/0929866054395879
---------- VANCOUVER ----------
Ayub, M.J., Barroso, J.A., Levin, M.J., Aguilar, C.F. Preliminary structural studies of the hydrophobic ribosomal P0 protein from Trypanosoma cruzi, a part of the P0/P1/P2 complex. Protein Pept. Lett. 2005;12(6):521-525.
http://dx.doi.org/10.2174/0929866054395879