Artículo

Berruezo, L.A.; Mercado Cárdenas, G.E.; Harries, E.D.M.; Stenglein, S.A.; Curti, R.N.; Rodriguero, M.S.; Galván, M.Z. "Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina" (2018) European Journal of Plant Pathology. 151(4):1065-1079
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Abstract:

Tobacco (Nicotiana tabacum L.) production is centred in the northwestern region of Argentina (NWA), where the incidence of root rot and stem diseases has increased considerably in recent years. This study aimed to analyse the genetic, morphological and pathogenic diversity of the Fusarium oxysporum and F. solani complexes (hereafter FOSC-FSSC), causing Fusarium wilt and root rot. One hundred tobacco fields were surveyed at six locations during two consecutive seasons, and 130 isolates were recovered from symptomatic tobacco plants. The isolates were characterized by morphological traits, molecular characteristics (EF1-α sequence) and pathogenicity tests. All of the isolates were identified as members of the FOSC or FSSC, exhibiting considerable intra-group variation. Three morphotypes were differentiated based on morphological characters in both complexes. The phylogenetic tree generated from the EF1-α sequences confirmed the isolates’ identification. The pathogenicity of the isolates towards tobacco seedlings was assessed in a greenhouse. Considerable variability in pathogenicity was observed among the isolates. Differences in the levels of pathogenicity were recorded. In the FOSC and FSSC, 81% and 60% of the isolates were pathogenic, respectively. In this study, members of FOSC and FSSC exhibited considerable variability in morphological characteristics and virulence, and a portion of them were non-pathogenic for tobacco. To the best of our knowledge, this study is the first to provide information on the variability of the pathogens associated with tobacco wilt and root rot in NWA. This work contributes to the development of sustainable management strategies in tobacco production. © 2018, Koninklijke Nederlandse Planteziektenkundige Vereniging.

Registro:

Documento: Artículo
Título:Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina
Autor:Berruezo, L.A.; Mercado Cárdenas, G.E.; Harries, E.D.M.; Stenglein, S.A.; Curti, R.N.; Rodriguero, M.S.; Galván, M.Z.
Filiación:Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), CCT-Salta. Leguizamón 366 1er Piso (4400), Salta, Argentina
Instituto Nacional de Tecnología Agropecuaria (INTA) EEA Salta, Ruta Nac. 68 km 172 (4403), Cerrillos, Salta, Argentina
Facultad de Ciencias Naturales, Universidad Nacional de Salta, Av. Bolivia 5150 (4400), Salta, Argentina
Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC-CONICET, Facultad de Agronomía de Azul, Área de Microbiología, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Republica de Italia 780. C.C. 47 (7300), Azul Provincia de Buenos Aires, Argentina
Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, IEGEBA (CONICET-UBA), Universidad de Buenos Aires, Intendente Güiraldes y Costanera Norte s/n, 4to. Piso, Pabellón II, Ciudad Universitaria, CI1428 EHA, Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:EF1-α; Fusarium root rot; Fusarium wilt; Nicotiana tabacum; Pathogenicity; disease incidence; fungal disease; fungus; genetic analysis; molecular analysis; morphology; pathogenicity; phylogenetics; root rot; symptom; tobacco; virulence; Argentina; Fusarium; Fusarium oxysporum; Fusarium solani; Nicotiana obtusifolia; Nicotiana tabacum
Año:2018
Volumen:151
Número:4
Página de inicio:1065
Página de fin:1079
DOI: http://dx.doi.org/10.1007/s10658-018-1443-6
Título revista:European Journal of Plant Pathology
Título revista abreviado:Eur. J. Plant Pathol.
ISSN:09291873
CODEN:EPLPE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09291873_v151_n4_p1065_Berruezo

Referencias:

  • Alves-Santos, F.M., Martinez-Bermejo, D., Rodrıguez-Molina, M.C., Dieza, J.J., Cultural characteristics, pathogenicity and genetic diversity of Fusarium oxysporum isolates from tobacco fields in Spain (2007) Physiological and Molecular Plant Pathology, 71 (1), pp. 26-32
  • Armstrong, G.M., Armstrong, J.K., Formae specials and races of Fusarium oxysporum causing a tracheomycosis in the syndrome diseases (1968) Phytopathology, 58 (9), pp. 1242-1246
  • Booth, C., (1971) The genus Fusarium, , Commonwealth Mycological Institute, Kew: 237p
  • Bueno, C.J., Fischer, I.H., Rosa, D.D., Firmino, A.C., Harakava, R., Oliveira, C.M.G., Furtado, E.L., Fusarium solani f. Sp. passiflorae: A new forma specialis causing collar rot in yellow passion fruit (2014) Plant Pathology, 63 (2), pp. 382-389
  • Chandra, N.S., Wulff, E.G., Udayashankar, A.C., Nandini, B.P., Niranjana, S.R., Mortensen, C.N., Prakash, H.S., Prospects of molecular markers in Fusarium species diversity (2011) Applied Microbiology and Biotechnology, 90 (5), pp. 1625-1639. , PID: 21494869
  • Chehri, K., Molecular identification of pathogenic Fusarium species, the causal agents of tomato wilt in western Iran (2016) Journal of Plant Protection Research, 56 (2), pp. 143-148
  • Chehri, K., Ghasempour, H.R., Karimi, N., Molecular phylogenetic and pathogenetic characterization of Fusarium solani species complex (FSSC), the cause of dry rot on potato in Iran (2014) Microbial Pathogenesis, 67, pp. 14-19. , PID: 24530481
  • Chehri, K., Salleh, B., Zakaria, L., Morphological and phylogenetic analysis of Fusarium solani species complex in Malaysia (2015) Microbial Ecology, 69 (3), pp. 457-471. , PID: 25238930
  • Chitrampalam, P., Nelson, B., Jr., Multilocus phylogeny reveals an association of agriculturally important Fusarium solani species complex (FSSC) 11, and clinically important FSSC 5 and FSSC 3+ 4 with soybean roots in the north central United States (2016) Antonie Van Leeuwenhoek, 109 (2), pp. 335-347. , PID: 26671414
  • Clark, C.A., Hyun, J.W., Hoy, M.W., Relationships among wilt-inducing isolates of Fusarium oxysporum from sweetpotato and tobacco (1998) Plant Disease, 82 (5), pp. 530-536
  • Costa, S.S., Matos, K.S., Tessmann, D.J., Seixas, C., Pfenning, L., Fusarium paranaense sp. nov., a member of the Fusarium solani species complex causes root rot on soybean in Brazil (2015) Fungal Biology, 120 (1), pp. 51-60. , PID: 26693684
  • Darriba, D., Taboada, G.L., Doallo, R., Posada, D., jModelTest 2: More models, new heuristics and parallel computing (2012) Natural Methods, 9 (8), p. 772
  • Di Rienzo, J.A., Casanoves, F., Balzarini, M.G., Gonzalez, L., Tablada, M., Robledo, C.W., (2017) InfoStat Grupo InfoStat, , http://www.infostat.com.ar, Universidad Nacional de Córdoba, Argentina, FCA
  • Fogliata, G., Martínez, C., Acosta, M., Muñoz, M., Ploper, L., First report of Fusarium rot caused by Fusarium oxysporum on lemon in Tucumán, Argentina (2013) Plant Disease, 97 (7), pp. 989-998
  • (2012) Production Crops, , http://www.fao.org/docrep/006/y4956e/y4956e04.htm#bm04.2, verified may, 2017
  • Fracchia, S., Garcia-Romera, I., Godeas, A., Ocampo, J.A., Effect of the saprophytic fungus Fusarium oxysporum on arbuscular mycorrhizal colonization and growth of plants in greenhouse and field trials (2000) Plant and Soil, 223 (1), pp. 177-186
  • Fujinaga, M., Ogiso, H., Shinohara, H., Tsushima, S., Nishimura, N., Togawa, M., Saito, H., Nozue, M., Phylogenetic relationships between the lettuce root rot pathogen Fusarium oxysporum f. Sp. lactucae races 1, 2, and 3 based on the sequence of the intergenic spacer region of its ribosomal DNA (2005) Journal of General Plant Pathology, 71 (6), pp. 402-407
  • Geiser, D.M., Jimenez-Gasco, M., Kang, S., Makalowska, I., Veeraraghavan, N., Ward, T.J., Zhang, N., O’Donnell, K., FUSARIUM-ID v. 1.0: A DNA sequence database for identifying fusarium (2004) European Journal of Plant Pathology, 110, pp. 473-479
  • Gimenez Monge, J.L., Mercado Cardenas, G., Lacci, F., Ortega, A., Arzeno, J.L., (2009) Un enfoque integral del amarillamiento del tabaco” Revista: Panorama Agropecuario Centro regional Salta y Jujuy Año10 N°52
  • Goloboff, P.A., Catalano, S.A., TNT version 1.5, including a full implementation of phylogenetic morphometrics (2016) Cladistics, 32, pp. 221-238
  • Guindon, S., Gascuel, O., A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood (2003) Systematic Biology, 52 (5), pp. 696-704. , PID: 14530136
  • Guindon, S., Dufayard, J.F., Lefort, V., Anisimova, M., Hordijk, W., Gascuel, O., New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0 (2010) Systematic Biology, 59 (3), pp. 307-321. , PID: 20525638
  • Hall, T., BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/ 98/NT (1999) Nucleic Acids Symposium Series, 41 (41), pp. 95-98
  • Huang, L.W., Wang, C.J., Lin, Y.S., Chung, W.C., Chung, W.H., Stem rot of jewel orchids caused by a new forma specialis, Fusarium oxysporum f. Sp. anoectochili in Taiwan (2014) Plant Pathology, 63 (3), pp. 539-547
  • Huelsenbeck, J.P., Ronquist, F., MrBayes 3: Bayesian phylogenetic inference under mixed models (2001) Bioinformatics, 19 (12), pp. 1572-1574
  • Johnson, J., Fusarium-wilt of tobacco (1921) Journal of Agricultural Research, 20 (1), pp. 515-535
  • Kistler, H.C., Genetic diversity in the plant-pathogenic fungus Fusarium oxysporum (1997) Phytopathology, 87 (4), pp. 474-479. , PID: 18945129
  • Koyyappurath, S., Atuahiva, T., Le Guen, R., Batina, H., Le Squin, S., Gautheron, N., Liew, E.C.Y., Fusarium oxysporum f. sp. radicis-vanillae is the causal agent of root and stem rot of vanilla (2016) Plant Pathology, 65 (1), pp. 612-625
  • LaMondia, J.A., Pathogenicity and vegetative compatibility of Fusarium oxysporum isolated from tobacco (1990) Tobacco International, 192 (22), pp. 58-61
  • LaMondia, J., Fusarium wilt of tobacco (2015) Crop Protection, 73, pp. 73-77
  • LaMondia, J.A., Taylor, G.S., Influence of the tobacco cyst nematode (Globodera tabacum) on fusarium wilt of Connecticut broadleaf tobacco (1987) Plant Disease, 71 (12), pp. 1129-1132
  • Laurence, M.H., Summerell, B.A., Burgess, L.W., Liew, E.C., Genealogical concordance phylogenetic species recognition in the Fusarium oxysporum species complex (2014) Fungal Biology, 118 (4), pp. 374-384. , PID: 24742832
  • Leslie, J., Summerell, S., (2006) The Fusarium laboratory manual, p. 388. , Ed. Blackwell Publi. Iowa
  • Leslie, J.F., Pearson, C.A., Nelson, P.E., Toussoun, T.A., Fusarium spp. From corn, sorghum, and soybean fields in the central and eastern United States (1990) Ecological Studies, 44 (63), p. 66
  • Lievens, B., Rep, M., Thomma, B., Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum (2008) Pest Managment Science, 64 (8), pp. 781-788
  • Lori, G., Edel-Hermann, V., Gautheron, N., Alabouvette, C., Genetic diversity of pathogenic and nonpathogenic populations of Fusarium oxysporum isolated from carnation fields in Argentina (2004) Phytopathology, 94 (6), pp. 661-668. , PID: 18943491
  • Lucas, G., (1975) Diseases of Tobacco, pp. 174-190. , Third edition. Biological consulting associates box 5726. Raleigh, North Carolina
  • Malbrán, L., Mourelos, C., Mitidieri, M., Ronco, B., Lori, G., Fusarium wilt of lettuce caused by Fusarium oxysporum f. Sp. lactucae in Argentina (2016) Plant Disease, 98 (9), pp. 1281-1292
  • Mantel, N., The detection of disease clustering and a generalizated regression approach (1967) Cancer Rasearch, 27, pp. 209-220
  • Mercado Cárdenas, G.E., Galván, M.Z., Barrera, V.A., Rodriguero, M.S., Carmona, M.A., March, G.J., Shew, H.D., Molecular identification and pathogenicity of Rhizoctonia spp. from tobacco growing areas in northwestern Argentina (2015) Tropical Plant Pathology, 40 (3), pp. 160-168
  • Michielse, C., Rep, M., Pathogen profile update: Fusarium oxysporum (2009) Molecular Plant Pathology, 10 (3), pp. 311-324. , PID: 19400835
  • Miniagri, (2016) Informe de productos regionales, , http://www.agroindustria.gob.ar/sitio/areas/tabaco/produccion_mercados/interno, Ministerio de Agroindustria, Presidencia de la Nación
  • Nalim, F., Samuels, G., Wijesundera, R.L., Geiser, D.M., New species from the Fusarium solani species complex derived from perithecia and soil in the old world tropics (2011) Mycologia, 103 (6), pp. 1302-1330. , PID: 21700636
  • O’Donnell, K., Kistler, H.C., Cigelnik, E., Ploetz, R.C., Multiple evolutionary origins of the fungus causing Panamá disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies (1998) Proceedings of the National Academy of Sciences, 95 (5), pp. 2044-2049
  • A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex (2009) Fungal Genetics and Biology, 46 (12), pp. 936-948
  • O'Donnell, K., Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex (2000) Mycologia, 92 (5), pp. 919-938
  • O'Donnell, K., Sutton, D.A., Fothergill, A., McCarthy, D., Rinaldi, M.G., Brandt, M.E., Geiser, D.M., Molecular phylogenetic diversity, multilocus haplotype nomenclature, and in vitro antifungal resistance within the Fusarium solani species complex (2008) Journal of Clinical Microbiology, 46 (8), pp. 2477-2490. , PID: 18524963
  • Pinaria, A.G., Laurence, M.H., Burgess, L.W., Liew, E.C.Y., Phylogeny and origin of Fusarium oxysporum f. Sp. vanillae in Indonesia (2015) Plant Pathology, 64 (6), pp. 1358-1365
  • Rodríguez-Molina, M.C., Morales-Rodríguez, M.C., Palo, C., Osuna, M.D., Iglesias, M.J., García, J.A., Pathogenicity, vegetative compatibility and RAPD analysis of Fusarium oxysporum isolates from tobacco fields in Extremadura (2013) European Journal of Plant Pathology, 136 (3), pp. 639-650
  • Ronquist, F., Huelsenbeck, J.P., MrBayes. 3: Bayesian phylogenetic inference under mixed models (2003) Bioinformatics, 19 (12), pp. 1572-1574. , PID: 12912839
  • Ronquist, F., Teslenko, M., Van der Mark, P., MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space (2012) Systematic Biology, 61 (3), pp. 539-542. , PID: 22357727
  • Scandiani, M., Carmona, M., Luque, M., Matos, K., Lenzi, L., Formento, A., Isolation, identification and damages associated with sudden death syndrome in soybean cultivation in Argentina (2012) Tropical Plant Pathology, 37 (5), pp. 358-362
  • Schroers, H.J., Samuels, G.J., Zhang, N., Short, D.P., Juba, J., Geiser, D.M., Epitypification of Fusisporium (Fusarium) solani and its assignment to a common phylogenetic species in the Fusarium solani species complex (2016) Mycologia, 108 (4), pp. 806-819. , PID: 27055573
  • Shew, H.D., Lucas, G.B., (1991) Compendium of tabacco diseases, , APS, St Paul
  • Short, D.P., O’Donnell, K., Thrane, U., Nielsen, K.F., Zhang, N., Juba, J.H., Geiser, D.M., Phylogenetic relationships among members of the Fusarium solani species complex in human infections and the descriptions of F. keratoplasticum sp. nov. and F. petroliphilum stat. Nov (2013) Fungal Genetics and Biology, 53, pp. 59-70. , PID: 23396261
  • Stenglein, S.A., Balatti, P.A., Genetic diversity of Phaeoisariopsis griseola in Argentina as revealed by virulence and molecular markers (2006) Physiological and Molecular Plant Pathology, 68 (4), pp. 158-167
  • Thompson, J.D., Higgins, D.G., Gibson, T.J., CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position- specific gap penalties and weight matrix choice (1994) Nucleic Acids Research, 22 (22), pp. 4673-4680. , PID: 7984417
  • Tjamos, S.E., Markakis, E.A., Antoniou, P., Paplomatas, E.J., First record of fusarium wilt of tobacco in Greece imported as seedborne inoculum (2006) Journal of Phytopathology, 154 (4), pp. 193-196
  • Zhang, N., O'Donnell, K., Sutton, D., Nalim, A., Summerbell, R., Padhye, A., Geiser, D., Members of the Fusarium solani species complex that cause infections in both humans and plants are common in the environment (2006) Journal of Clinical Microbiology, 44 (6), pp. 2186-2190. , PID: 16757619

Citas:

---------- APA ----------
Berruezo, L.A., Mercado Cárdenas, G.E., Harries, E.D.M., Stenglein, S.A., Curti, R.N., Rodriguero, M.S. & Galván, M.Z. (2018) . Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina. European Journal of Plant Pathology, 151(4), 1065-1079.
http://dx.doi.org/10.1007/s10658-018-1443-6
---------- CHICAGO ----------
Berruezo, L.A., Mercado Cárdenas, G.E., Harries, E.D.M., Stenglein, S.A., Curti, R.N., Rodriguero, M.S., et al. "Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina" . European Journal of Plant Pathology 151, no. 4 (2018) : 1065-1079.
http://dx.doi.org/10.1007/s10658-018-1443-6
---------- MLA ----------
Berruezo, L.A., Mercado Cárdenas, G.E., Harries, E.D.M., Stenglein, S.A., Curti, R.N., Rodriguero, M.S., et al. "Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina" . European Journal of Plant Pathology, vol. 151, no. 4, 2018, pp. 1065-1079.
http://dx.doi.org/10.1007/s10658-018-1443-6
---------- VANCOUVER ----------
Berruezo, L.A., Mercado Cárdenas, G.E., Harries, E.D.M., Stenglein, S.A., Curti, R.N., Rodriguero, M.S., et al. Characterization of Fusarium species associated with tobacco diseases in Northwestern Argentina. Eur. J. Plant Pathol. 2018;151(4):1065-1079.
http://dx.doi.org/10.1007/s10658-018-1443-6