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Lopez Bergami, P.; Cabeza Meckert, P.; Kaplan, D.; Levitus, G.; Elias, F.; Quintana, F.; Van Regenmortel, M.; Laguens, R.; Levin, M.J. "Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice" (1997) FEMS Immunology and Medical Microbiology. 18(1):75-85
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Abstract:

Molecular expression cloning techniques revealed that patients with severe chronic Chagas heart disease showed a strong humoral response against the cloned C-terminal portion of the Trypanosoma cruzi ribosomal P2β protein, previously named JL5. The main linear epitope of this polypeptide was mapped to the 13 C-terminal amino acid sequence EEEDDDMGFGLFD (named R13), which is almost identical to the mammalian ribosomal P consensus sequence EESDDDMGFGLFD (named H13). Enzyme-linked immunosorbent assay measurements demonstrated that sera from patients with chronic Chagas heart disease presented a very specific anti-P humoral response with high anti- R13, but low H13 antibody levels. We attempted to develop an animal model that would reproduce, at least partially, two features of the human infection: (1) the serological pattern of the anti-P response, and (2) specific cardiac symptoms. To this effect, mice were immunized with T. cruzi P2β recombinant protein. Immunization reproduced the typical anti-P antibody profile defined for chronic infections, but did not induce cardiac inflammatory lesions. However, it altered significantly the electrocardiograms of immunized mice. It is suggested that this assay represents a functional test for assessing the biological activity of antibodies against T. cruzi ribosomal P protein on cardiac muscle.

Registro:

Documento: Artículo
Título:Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice
Autor:Lopez Bergami, P.; Cabeza Meckert, P.; Kaplan, D.; Levitus, G.; Elias, F.; Quintana, F.; Van Regenmortel, M.; Laguens, R.; Levin, M.J.
Filiación:Inst. Invest. Ing. Genet. y Biol. M., Obligado 2490, 1428 Buenos Aires, Argentina
Inst. Cardiol. y Cirugia Cardiovasc., Fundación Favaloro, Belgrano 1746, 1093 Buenos Aires, Argentina
Comn. Invest. Cie. Provincia B., Buenos Aires, Argentina
Deutsches RheumaForschungsZentrum, Monbijoustraße 2A, D-10117 Berlin, Germany
Inst. de Biologie Moléculaire, Cellulaire UPR 9021 CNRS, 67084 Strasbourg Cedex, France
Palabras clave:Chagas disease; Electrocardiogram; Functional assay; Heart; Immunization; Ribosomal P protein; Trypanosoma cruzi; animal model; article; chagas disease; electrocardiogram; enzyme linked immunosorbent assay; human; humoral immunity; immunization; mouse; nonhuman; priority journal; trypanosoma cruzi; Animals; Antibodies, Protozoan; Autoantibodies; Carrier Proteins; Electrocardiography; Heart; Humans; Immunization; Mice; Mice, Inbred BALB C; Myocardium; Protozoan Proteins; Recombinant Fusion Proteins; Ribosomal Proteins; Trypanosoma cruzi
Año:1997
Volumen:18
Número:1
Página de inicio:75
Página de fin:85
DOI: http://dx.doi.org/10.1016/S0928-8244(97)00026-6
Título revista:FEMS Immunology and Medical Microbiology
Título revista abreviado:FEMS IMMUNOL. MED. MICROBIOL.
ISSN:09288244
CODEN:FIMIE
CAS:Antibodies, Protozoan; Autoantibodies; Carrier Proteins; L12E protein, Trypanosoma cruzi; maltose-binding protein; Protozoan Proteins; Recombinant Fusion Proteins; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09288244_v18_n1_p75_LopezBergami

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Citas:

---------- APA ----------
Lopez Bergami, P., Cabeza Meckert, P., Kaplan, D., Levitus, G., Elias, F., Quintana, F., Van Regenmortel, M.,..., Levin, M.J. (1997) . Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice. FEMS Immunology and Medical Microbiology, 18(1), 75-85.
http://dx.doi.org/10.1016/S0928-8244(97)00026-6
---------- CHICAGO ----------
Lopez Bergami, P., Cabeza Meckert, P., Kaplan, D., Levitus, G., Elias, F., Quintana, F., et al. "Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice" . FEMS Immunology and Medical Microbiology 18, no. 1 (1997) : 75-85.
http://dx.doi.org/10.1016/S0928-8244(97)00026-6
---------- MLA ----------
Lopez Bergami, P., Cabeza Meckert, P., Kaplan, D., Levitus, G., Elias, F., Quintana, F., et al. "Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice" . FEMS Immunology and Medical Microbiology, vol. 18, no. 1, 1997, pp. 75-85.
http://dx.doi.org/10.1016/S0928-8244(97)00026-6
---------- VANCOUVER ----------
Lopez Bergami, P., Cabeza Meckert, P., Kaplan, D., Levitus, G., Elias, F., Quintana, F., et al. Immunization with recombinant Trypanosoma cruzi ribosomal P2β protein induces changes in the electrocardiogram of immunized mice. FEMS IMMUNOL. MED. MICROBIOL. 1997;18(1):75-85.
http://dx.doi.org/10.1016/S0928-8244(97)00026-6