Abstract:
The objective of this work is to test the performance of new synthetic polydimethylsiloxane (PDMS)-based bed particles acting as carriers for bacteria biofilms. The particles obtained have a highly interconnected porous structure which offers a large surface adsorption area to the bacteria. In addition, PDMS materials can be cross-linked by copolymerization with other polymers. In the present work we have chosen two hydrophilic polymers: xanthan gum polysaccharide and tetraethoxysilane (TEOS). This versatile composition helps to modulate the interfacial hydrophobic/hydrophilic balance at the particle surface level and the roughness topology and pore size distribution, as revealed by scanning electron microscopy. Biofilm formation of a consortium isolated from a tannery effluent enriched in Sulphate Reducing Bacteria (SRB), and pure Acidithiobacillus ferrooxidans (AF) strains were assayed in three different bed particles synthesized with pure PDMS, PDMS-xanthan gum and PDMS-TEOS hybrids. Bacterial viability assays using confocal laser scanning fluorescence microscopy indicate that inclusion of hydrophilic groups on particle's surface significantly improves both cell adhesion and viability. © 2010 Elsevier B.V.
Registro:
Documento: |
Artículo
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Título: | PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition |
Autor: | Fernández, M.R.; Casabona, M.G.; Anupama, V.N.; Krishnakumar, B.; Curutchet, G.A.; Bernik, D.L. |
Filiación: | Instituto de Química Física de Materiales, Ambiente y Energía (INQUIMAE), Departamento de Química Inorgánica, Analítica y Química Física, Facultad de Ciencias Exactas y Naturales, Argentina Environmental Technology, National Institute for Interdisciplinary Science, Technology (NIST, CSIR-India), Thiruvananthapuram 695019, India Escuela de Ciencia y Tecnología, Universidad Nacional de San Martín, San Martín, Provincia de Buenos Aires, Argentina
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Palabras clave: | Bed material; Biofilm; CSLM; PDMS; SEM; Acidithiobacillus ferrooxidans; Bacterial biofilm; Bacterial viability; Bed materials; Bed particles; Biofilm formation; Confocal laser scanning; CSLM; Hydrophilic groups; Hydrophilic polymers; Interconnected porous structure; Particle surface; PDMS; Polydimethylsiloxane PDMS; Polymer composition; Porous particle; SEM; Sulphate-reducing bacteria; Surface adsorption; Tannery effluent; Tetraethoxysilanes; Xanthan Gum; Adhesion; Adsorption; Bacteriology; Biofilms; Biofilters; Cell adhesion; Cell immobilization; Fluorescence microscopy; Hydrophilicity; Microchannels; Polymers; Pore size; Porous materials; Scanning electron microscopy; Silicones; Surfaces; dimeticone; tetraethoxysilane; xanthan; Acidithiobacillus ferrooxidans; article; bacterial membrane; bacterial strain; bacterial viability; bacterium adherence; bacterium culture; biofilm; controlled study; hydrophilicity; hydrophobicity; immobilized cell; membrane formation; membrane permeability; nonhuman; polymerization; porosity; priority journal; scanning electron microscopy; sulfate reducing bacterium; surface property; Acidithiobacillus; Bacteria; Bacterial Adhesion; Biofilms; Cells, Immobilized; Dimethylpolysiloxanes; Hydrophobic and Hydrophilic Interactions; Microscopy, Confocal; Microscopy, Electron, Scanning; Microscopy, Fluorescence; Particle Size; Polysaccharides, Bacterial; Porosity; Silanes; Sulfates; Surface Properties; Acidithiobacillus ferrooxidans; Bacteria (microorganisms) |
Año: | 2010
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Volumen: | 81
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Número: | 1
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Página de inicio: | 289
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Página de fin: | 296
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DOI: |
http://dx.doi.org/10.1016/j.colsurfb.2010.07.018 |
Título revista: | Colloids and Surfaces B: Biointerfaces
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Título revista abreviado: | Colloids Surf. B Biointerfaces
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ISSN: | 09277765
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CODEN: | CSBBE
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CAS: | dimeticone, 32028-95-8, 68248-27-1, 9004-73-3, 9006-65-9; xanthan, 11138-66-2; Dimethylpolysiloxanes; Polysaccharides, Bacterial; Silanes; Sulfates; baysilon, 63148-62-9; tetraethoxysilane, 78-10-4; xanthan gum, 11138-66-2
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09277765_v81_n1_p289_Fernandez |
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Citas:
---------- APA ----------
Fernández, M.R., Casabona, M.G., Anupama, V.N., Krishnakumar, B., Curutchet, G.A. & Bernik, D.L.
(2010)
. PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition. Colloids and Surfaces B: Biointerfaces, 81(1), 289-296.
http://dx.doi.org/10.1016/j.colsurfb.2010.07.018---------- CHICAGO ----------
Fernández, M.R., Casabona, M.G., Anupama, V.N., Krishnakumar, B., Curutchet, G.A., Bernik, D.L.
"PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition"
. Colloids and Surfaces B: Biointerfaces 81, no. 1
(2010) : 289-296.
http://dx.doi.org/10.1016/j.colsurfb.2010.07.018---------- MLA ----------
Fernández, M.R., Casabona, M.G., Anupama, V.N., Krishnakumar, B., Curutchet, G.A., Bernik, D.L.
"PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition"
. Colloids and Surfaces B: Biointerfaces, vol. 81, no. 1, 2010, pp. 289-296.
http://dx.doi.org/10.1016/j.colsurfb.2010.07.018---------- VANCOUVER ----------
Fernández, M.R., Casabona, M.G., Anupama, V.N., Krishnakumar, B., Curutchet, G.A., Bernik, D.L. PDMS-based porous particles as support beds for cell immobilization: Bacterial biofilm formation as a function of porosity and polymer composition. Colloids Surf. B Biointerfaces. 2010;81(1):289-296.
http://dx.doi.org/10.1016/j.colsurfb.2010.07.018