Artículo

Bellesi, F.A.; Ruiz-Henestrosa, V.M.P.; Maldonado-Valderrama, J.; Del Castillo Santaella, T.; Pilosof, A.M.R. "Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films" (2018) Colloids and Surfaces B: Biointerfaces. 161:547-554
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Abstract:

The behaviour of proteins (β-lactoglobulin (βlg) and soy protein isolate (SPI)) and a surface active polysaccharide (hydroxypropylmethylcellulose, HPMC) o/w interfacial films under simulated gastrointestinal conditions using the interfacial tensiometer Octopus were compared and related to the performance of the emulsions (using the same emulsifiers) under in vitro digestion. The evolution of interfacial tension (γ) was used to investigate the effect of gastrointestinal fluids on o/w interfacial films. Clear differences were observed among these emulsifiers. During the gastric phase, HPMC showed the lowest change in γ values as compared to protein films. The most important changes occurred during the intestinal stage where it was observed an important decrease of γ associated with the rapid penetration of BS, followed by a lower rate of decrease attributable to the accumulation of FFA at the interface. In the last stage, the subphase was exchanged by buffer alone, to remove the reversibly adsorbed digestion products. SPI formed the most resistant interface to the remotion of digestion products, followed by HPMC and finally by βlg. The results agree with the degree of lipolysis reported for the emulsions stabilized by these emulsifiers, which suggest that lipid digestion could be modulated by the ability of emulsifiers to prevent the BS activity (to adsorb at the O/W interface or remove the inhibitory digestion products from the interface). Thus, emulsifiers-BS interactions appears as a key factor in controlling the lipolysis. © 2017 Elsevier B.V.

Registro:

Documento: Artículo
Título:Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films
Autor:Bellesi, F.A.; Ruiz-Henestrosa, V.M.P.; Maldonado-Valderrama, J.; Del Castillo Santaella, T.; Pilosof, A.M.R.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria (1428), Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina
Grupo de física de Fluidos, Departamento de Física Aplicada, Universidad de Granada, Av. Fuentenueva S/N, Granada, 18071, Spain
Palabras clave:Bile salts; Digestion; Interface; Lipolysis; Interfaces (materials); Salts; Sludge digestion; Beta-lactoglobulin; Bile salts; Digestion products; Hydroxypropyl methylcellulose; In-vitro digestions; Lipolysis; Simulated gastrointestinal conditions; Soy protein isolates; Enzymes; beta lactoglobulin; hydroxypropylmethylcellulose; soybean protein; hydroxypropylmethylcellulose; lactoglobulin; oil; polysaccharide; soybean protein; water; analysis of variance; Article; desorption; emulsion; hydrolysis; in vitro study; lipolysis; micelle; molecular dynamics; molecular interaction; priority journal; protein denaturation; protein metabolism; protein structure; comparative study; digestion; gastrointestinal tract; intestine; intestine absorption; manometry; metabolism; procedures; surface tension; Digestion; Emulsions; Gastrointestinal Tract; Hypromellose Derivatives; Intestinal Absorption; Intestines; Lactoglobulins; Lipolysis; Manometry; Oils; Polysaccharides; Soybean Proteins; Surface Tension; Water
Año:2018
Volumen:161
Página de inicio:547
Página de fin:554
DOI: http://dx.doi.org/10.1016/j.colsurfb.2017.11.027
Título revista:Colloids and Surfaces B: Biointerfaces
Título revista abreviado:Colloids Surf. B Biointerfaces
ISSN:09277765
CODEN:CSBBE
CAS:beta lactoglobulin, 9045-23-2; hydroxypropylmethylcellulose, 9004-65-3; soybean protein, 9010-10-0; water, 7732-18-5; Emulsions; Hypromellose Derivatives; Lactoglobulins; Oils; Polysaccharides; Soybean Proteins; Water
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09277765_v161_n_p547_Bellesi

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Citas:

---------- APA ----------
Bellesi, F.A., Ruiz-Henestrosa, V.M.P., Maldonado-Valderrama, J., Del Castillo Santaella, T. & Pilosof, A.M.R. (2018) . Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films. Colloids and Surfaces B: Biointerfaces, 161, 547-554.
http://dx.doi.org/10.1016/j.colsurfb.2017.11.027
---------- CHICAGO ----------
Bellesi, F.A., Ruiz-Henestrosa, V.M.P., Maldonado-Valderrama, J., Del Castillo Santaella, T., Pilosof, A.M.R. "Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films" . Colloids and Surfaces B: Biointerfaces 161 (2018) : 547-554.
http://dx.doi.org/10.1016/j.colsurfb.2017.11.027
---------- MLA ----------
Bellesi, F.A., Ruiz-Henestrosa, V.M.P., Maldonado-Valderrama, J., Del Castillo Santaella, T., Pilosof, A.M.R. "Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films" . Colloids and Surfaces B: Biointerfaces, vol. 161, 2018, pp. 547-554.
http://dx.doi.org/10.1016/j.colsurfb.2017.11.027
---------- VANCOUVER ----------
Bellesi, F.A., Ruiz-Henestrosa, V.M.P., Maldonado-Valderrama, J., Del Castillo Santaella, T., Pilosof, A.M.R. Comparative interfacial in vitro digestion of protein and polysaccharide oil/water films. Colloids Surf. B Biointerfaces. 2018;161:547-554.
http://dx.doi.org/10.1016/j.colsurfb.2017.11.027