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Abstract:

The broad-spectrum herbicide glyphosate (N - phosphonomethylglicine) is the most common pesticide used in the Humid Pampa, the main agricultural region in Argentina. According to agronomical practices and topogeographical characteristics of the region, rhizoremediation arises as a promising technology to mitigate glyphosate impact on health and agroecosystems. Lotus corniculatus L. (birdsfoot trefoil) and Lotus tenuis Waldst. et Kit. (=Lotus glaber Mill., narrowleaf trefoil) were selected to carry out tolerance studies as the starting point of a rhizoremediation process. L. corniculatus presented the highest root and foliar tolerance to glyphosate, corresponding to 5.0 mg kg-1 and 700 g ha-1 respectively. The enzyme enolpyruvylshikimate-3-phosphate synthase (EPSP synthase) partial cDNA sequence and whole plant shikimate accumulation assay were performed on L. corniculatus in order investigate tolerance mechanisms. No amino acid substitution related to glyphosate tolerance was found on EPSP synthase cDNA sequence. The shikimate accumulation study indicates that limited uptake and/or translocation of the herbicide is the most probable tolerance mechanism. Results obtained in this study, plus the productive and adaptive advantages of L. corniculatus make it a valuable candidate to develop rhizoremediation strategies. © 2016 Elsevier B.V.

Registro:

Documento: Artículo
Título:Assessing the glyphosate tolerance of Lotus corniculatus and L. tenuis to perform rhizoremediation strategies in the Humid Pampa (Argentina)
Autor:Massot, F.; Smith, M.E.; Vitali, V.A.; Giulietti, A.M.; Merini, L.J.
Filiación:Cátedra de Microbiología Industrial y Biotecnología, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Argentina
Instituto de Biodiversidad y Biología Experimental y Aplicada, Universidad de Buenos Aires, Consejo Nacional de Investigaciones Científicas y Tecnológicas, Argentina
Laboratorio de Malezas y Herbicidas, Instituto Nacional de Tecnología Agropecuaria, Estación Experimental Agropecuaria Anguil, Argentina
Palabras clave:Agroecosystems; Glyphosate; Humid Pampa region; Lotus corniculatus; Lotus tenuis; Rhizoremediation; Ecosystems; Weed control; Agro-ecosystems; Glyphosates; Humid Pampa region; Lotus corniculatus; Lotus tenuis; Rhizoremediation; Herbicides; agricultural ecosystem; bioaccumulation; bioassay; biodegradation; enzyme activity; glyphosate; herb; microbial activity; tolerance; Argentina; Pampas; Lotus corniculatus; Lotus glaber; Lotus oblongifolius; Lotus tenuis
Año:2016
Volumen:90
Página de inicio:392
Página de fin:398
DOI: http://dx.doi.org/10.1016/j.ecoleng.2016.01.031
Título revista:Ecological Engineering
Título revista abreviado:Ecol. Eng.
ISSN:09258574
CODEN:ECENE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09258574_v90_n_p392_Massot

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Citas:

---------- APA ----------
Massot, F., Smith, M.E., Vitali, V.A., Giulietti, A.M. & Merini, L.J. (2016) . Assessing the glyphosate tolerance of Lotus corniculatus and L. tenuis to perform rhizoremediation strategies in the Humid Pampa (Argentina). Ecological Engineering, 90, 392-398.
http://dx.doi.org/10.1016/j.ecoleng.2016.01.031
---------- CHICAGO ----------
Massot, F., Smith, M.E., Vitali, V.A., Giulietti, A.M., Merini, L.J. "Assessing the glyphosate tolerance of Lotus corniculatus and L. tenuis to perform rhizoremediation strategies in the Humid Pampa (Argentina)" . Ecological Engineering 90 (2016) : 392-398.
http://dx.doi.org/10.1016/j.ecoleng.2016.01.031
---------- MLA ----------
Massot, F., Smith, M.E., Vitali, V.A., Giulietti, A.M., Merini, L.J. "Assessing the glyphosate tolerance of Lotus corniculatus and L. tenuis to perform rhizoremediation strategies in the Humid Pampa (Argentina)" . Ecological Engineering, vol. 90, 2016, pp. 392-398.
http://dx.doi.org/10.1016/j.ecoleng.2016.01.031
---------- VANCOUVER ----------
Massot, F., Smith, M.E., Vitali, V.A., Giulietti, A.M., Merini, L.J. Assessing the glyphosate tolerance of Lotus corniculatus and L. tenuis to perform rhizoremediation strategies in the Humid Pampa (Argentina). Ecol. Eng. 2016;90:392-398.
http://dx.doi.org/10.1016/j.ecoleng.2016.01.031