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Verneri, P.; Romero, J.J.; De Rossi, M.C.; Alvarez, Y.; Oses, C.; Guberman, A.; Levi, V. "Imaging transcription factors dynamics with advanced fluorescence microscopy methods" (2018) Mechanisms of Development. 154:60-63
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Abstract:

Pluripotent stem cells (PSCs) are capable of self-renewing and producing all cell types derived from the three germ layers in response to developmental cues, constituting an important promise for regenerative medicine. Pluripotency depends on specific transcription factors (TFs) that induce genes required to preserve the undifferentiated state and repress other genes related to differentiation. The transcription machinery and regulatory components such as TFs are recruited dynamically on their target genes making it essential exploring their dynamics in living cells to understand the transcriptional output. Non-invasive and very sensitive fluorescence microscopy methods are making it possible visualizing the dynamics of TFs in living specimens, complementing the information extracted from studies in fixed specimens and bulk assays. In this work, we briefly describe the basis of these microscopy methods and review how they contributed to our knowledge of the function of TFs relevant to embryo development and cell differentiation in a variety of systems ranging from single cells to whole organisms. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Imaging transcription factors dynamics with advanced fluorescence microscopy methods
Autor:Verneri, P.; Romero, J.J.; De Rossi, M.C.; Alvarez, Y.; Oses, C.; Guberman, A.; Levi, V.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
CONICET–Universidad de Buenos Aires, Instituto de Química Biológica (IQUIBICEN), Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:transcription factor; cell differentiation; cell fate; cell nucleus; cell population; cell proliferation; cell self-renewal; chromatin immunoprecipitation; embryo development; fluorescence microscopy; fluorescence recovery after photobleaching; gene expression; gene targeting; molecular dynamics; nonhuman; pluripotent stem cell; priority journal; Review
Año:2018
Volumen:154
Página de inicio:60
Página de fin:63
DOI: http://dx.doi.org/10.1016/j.mod.2018.05.003
Título revista:Mechanisms of Development
Título revista abreviado:Mech. Dev.
ISSN:09254773
CODEN:MEDVE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09254773_v154_n_p60_Verneri

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Citas:

---------- APA ----------
Verneri, P., Romero, J.J., De Rossi, M.C., Alvarez, Y., Oses, C., Guberman, A. & Levi, V. (2018) . Imaging transcription factors dynamics with advanced fluorescence microscopy methods. Mechanisms of Development, 154, 60-63.
http://dx.doi.org/10.1016/j.mod.2018.05.003
---------- CHICAGO ----------
Verneri, P., Romero, J.J., De Rossi, M.C., Alvarez, Y., Oses, C., Guberman, A., et al. "Imaging transcription factors dynamics with advanced fluorescence microscopy methods" . Mechanisms of Development 154 (2018) : 60-63.
http://dx.doi.org/10.1016/j.mod.2018.05.003
---------- MLA ----------
Verneri, P., Romero, J.J., De Rossi, M.C., Alvarez, Y., Oses, C., Guberman, A., et al. "Imaging transcription factors dynamics with advanced fluorescence microscopy methods" . Mechanisms of Development, vol. 154, 2018, pp. 60-63.
http://dx.doi.org/10.1016/j.mod.2018.05.003
---------- VANCOUVER ----------
Verneri, P., Romero, J.J., De Rossi, M.C., Alvarez, Y., Oses, C., Guberman, A., et al. Imaging transcription factors dynamics with advanced fluorescence microscopy methods. Mech. Dev. 2018;154:60-63.
http://dx.doi.org/10.1016/j.mod.2018.05.003