Artículo

Solari, C.; Petrone, M.V.; Vazquez Echegaray, C.; Cosentino, M.S.; Waisman, A.; Francia, M.; Barañao, L.; Miriuka, S.; Guberman, A. "Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells" (2018) Mechanisms of Development. 154:116-121
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Abstract:

Redox homeostasis is vital for cellular functions and to prevent the detrimental consequences of oxidative stress. Pluripotent stem cells (PSCs) have an enhanced antioxidant system which supports the preservation of their genome. Besides, reactive oxygen species (ROS) are proposed to be involved in both self-renewal maintenance and in differentiation in embryonic stem cells (ESCs). Increasing evidence shows that cellular systems related to the oxidative stress defense decline along differentiation of PSCs. Although redox homeostasis has been extensively studied for many years, the knowledge about the transcriptional regulation of the genes involved in these systems is still limited. In this work, we studied Sod1 gene modulation by the PSCs fundamental transcription factors Oct4, Sox2 and Nanog. We found that this gene, which is expressed in mouse ESCs (mESCs), was repressed when they were induced to differentiate. Accordingly, these factors induced Sod1 promoter activity in a trans-activation assay. Finally, Sod1 mRNA levels were reduced when Oct4, Sox2 and Nanog were down-regulated by a shRNA approach in mESCs. Taken together, we found that PSCs’ key transcription factors are involved in the modulation of Sod1 gene, suggesting a relationship between the pluripotency core and redox homeostasis in these cells. © 2018 Elsevier B.V.

Registro:

Documento: Artículo
Título:Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells
Autor:Solari, C.; Petrone, M.V.; Vazquez Echegaray, C.; Cosentino, M.S.; Waisman, A.; Francia, M.; Barañao, L.; Miriuka, S.; Guberman, A.
Filiación:Laboratorio de Regulación Génica en Células Madre, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Química Biológica (IQUIBICEN), Universidad de Buenos Aires - CONICET, Buenos Aires, Argentina
Laboratorio de Investigación de Aplicación a Neurociencias (LIAN), Fundación para la Lucha contra las Enfermedades Neurológicas de la Infancia (FLENI) – CONICET, Buenos Aires, Argentina
Departamento de Fisiología y Biología Molecular y Celular, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Antioxidant defense system; Cu-Zn Sod; Sod1; Transcriptional regulation; copper zinc superoxide dismutase; messenger RNA; mitochondrial DNA; octamer transcription factor 4; short hairpin RNA; transcription factor NANOG; transcription factor Sox2; Article; binding site; blastocyst; cardiac muscle cell; cell differentiation; cell growth; cell nucleus; cell self-renewal; controlled study; cytoplasm; down regulation; embryonic stem cell; gene; gene expression; gene mutation; human; human cell; in vitro study; nerve cell; oxidative stress; priority journal; promoter region; protein expression; protein targeting; smooth muscle cell; sod1 gene; transcription regulation
Año:2018
Volumen:154
Página de inicio:116
Página de fin:121
DOI: http://dx.doi.org/10.1016/j.mod.2018.06.004
Título revista:Mechanisms of Development
Título revista abreviado:Mech. Dev.
ISSN:09254773
CODEN:MEDVE
CAS:copper zinc superoxide dismutase, 149394-67-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09254773_v154_n_p116_Solari

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Citas:

---------- APA ----------
Solari, C., Petrone, M.V., Vazquez Echegaray, C., Cosentino, M.S., Waisman, A., Francia, M., Barañao, L.,..., Guberman, A. (2018) . Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells. Mechanisms of Development, 154, 116-121.
http://dx.doi.org/10.1016/j.mod.2018.06.004
---------- CHICAGO ----------
Solari, C., Petrone, M.V., Vazquez Echegaray, C., Cosentino, M.S., Waisman, A., Francia, M., et al. "Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells" . Mechanisms of Development 154 (2018) : 116-121.
http://dx.doi.org/10.1016/j.mod.2018.06.004
---------- MLA ----------
Solari, C., Petrone, M.V., Vazquez Echegaray, C., Cosentino, M.S., Waisman, A., Francia, M., et al. "Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells" . Mechanisms of Development, vol. 154, 2018, pp. 116-121.
http://dx.doi.org/10.1016/j.mod.2018.06.004
---------- VANCOUVER ----------
Solari, C., Petrone, M.V., Vazquez Echegaray, C., Cosentino, M.S., Waisman, A., Francia, M., et al. Superoxide dismutase 1 expression is modulated by the core pluripotency transcription factors Oct4, Sox2 and Nanog in embryonic stem cells. Mech. Dev. 2018;154:116-121.
http://dx.doi.org/10.1016/j.mod.2018.06.004