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Abstract:

The anti-HSV-1 activity of meliacine (MA), a peptide isolated from leaves of Melia azedarach L., alone and in combination with acyclovir (ACV), was assayed against thymidine kinase-deficient (TK-) virus yields in vitro. MA alone proved to inhibit significantly TK- viral replication, whereas ACV was more potent than MA as an inhibitor of TK+ replication. TK- and TK+ synergistic inhibition by the combination of both agents was observed at concentrations that did not alter cell viability. The interaction between MA and ACV was quantitatively determined by calculating the combination index and plotting the data by the isobologram method. Besides, MA and ACV were able to suppress synergistically the antigen expression on HSV-1 infected cells processed by an immunofluorescence assay. These in vitro findings suggest that combinations of MA and ACV at appropriate doses may provide an increased efficacy in inhibiting both TK- and TK+ HSV-1 multiplication.

Registro:

Documento: Artículo
Título:Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir
Autor:Barquero, A.A.; Alché, L.E.; Coto, C.E.
Filiación:Laboratory of Virology, Department of Biochemistry, University of Buenos Aires, Pabellón II, Piso 4to., 1428 Buenos Aires, Argentina
Palabras clave:Acyclovir; Drug combination; Herpes simplex virus type 12; Meliacine; aciclovir; antivirus agent; melia azedarach extract; meliacine; peptide; plant extract; thymidine kinase; unclassified drug; animal cell; antigen expression; antiviral activity; article; cell viability; concentration response; controlled study; cytotoxicity; drug isolation; drug potentiation; enzyme deficiency; herpes simplex virus 1; immunofluorescence test; nonhuman; plant leaf; priority journal; vero cell; virus mutant; virus replication
Año:1997
Volumen:9
Número:1
Página de inicio:49
Página de fin:55
DOI: http://dx.doi.org/10.1016/S0924-8579(97)00023-X
Título revista:International Journal of Antimicrobial Agents
Título revista abreviado:INT. J. ANTIMICROB. AGENTS
ISSN:09248579
CODEN:IAAGE
CAS:aciclovir, 59277-89-3; thymidine kinase, 9002-06-6, 9086-73-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v9_n1_p49_Barquero

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Citas:

---------- APA ----------
Barquero, A.A., Alché, L.E. & Coto, C.E. (1997) . Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir. International Journal of Antimicrobial Agents, 9(1), 49-55.
http://dx.doi.org/10.1016/S0924-8579(97)00023-X
---------- CHICAGO ----------
Barquero, A.A., Alché, L.E., Coto, C.E. "Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir" . International Journal of Antimicrobial Agents 9, no. 1 (1997) : 49-55.
http://dx.doi.org/10.1016/S0924-8579(97)00023-X
---------- MLA ----------
Barquero, A.A., Alché, L.E., Coto, C.E. "Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir" . International Journal of Antimicrobial Agents, vol. 9, no. 1, 1997, pp. 49-55.
http://dx.doi.org/10.1016/S0924-8579(97)00023-X
---------- VANCOUVER ----------
Barquero, A.A., Alché, L.E., Coto, C.E. Antiviral activity of meliacine on the replication of a thymidine kinase-deficient mutant of Herpes simplex virus type 1 alone and in combination with acyclovir. INT. J. ANTIMICROB. AGENTS. 1997;9(1):49-55.
http://dx.doi.org/10.1016/S0924-8579(97)00023-X