Artículo

Todorov, S.D.; Wachsman, M.B.; Knoetze, H.; Meincken, M.; Dicks, L.M.T. "An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans" (2005) International Journal of Antimicrobial Agents. 25(6):508-513
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Abstract:

Enterococcus mundtii ST4V, isolated from soya beans, produces a 3950 Da antibacterial peptide active against Gram-positive and Gram-negative bacteria, including Enterococcus faecalis, Streptococcus spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, Streptococcus pneumoniae and Staphylococcus aureus. The peptide also inactivated the herpes simplex viruses HSV-1 (strain F) and HSV-2 (strain G), a polio virus (PV3, strain Sabin) and a measles virus (strain MV/BRAZIL/001/91, an attenuated strain of MV). MV, HSV-1 and HSV-2 were 95.5%-99.9% inactivated by peptide ST4V at 400 μg/ml. Monkey kidney Vero cells were not inactivated, even at four times the level peptide ST4V displayed antiviral activity, indicating that the effect was not due to cytotoxicity. Complete inactivation or significant reduction in antimicrobial activity was observed after treatment of peptide ST4V with Proteinase K, pronase, pepsin and trypsin. No change in antimicrobial activity was recorded after treatment with α-amylase, suggesting that peptide ST4V was not glycosylated. This is the first description of an antibacterial and antiviral peptide with such broad-spectrum of activity, produced by a lactic acid bacterium. © 2005 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.

Registro:

Documento: Artículo
Título:An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans
Autor:Todorov, S.D.; Wachsman, M.B.; Knoetze, H.; Meincken, M.; Dicks, L.M.T.
Filiación:Department of Microbiology, Stellenbosch University, 7600 Stellenbosch, South Africa
Laboratorio de Virologia, Departamento de Quimica Biologica, Pabellon 2, 1428 Buenos Aires, Argentina
Institute for Polimer Science, Stellenbosch University, 7600 Stellenbosch, South Africa
Palabras clave:Antibacterial and antiviral peptide; Enterococcus mundtii; amylase; pepsin A; peptide ST4V; polypeptide antibiotic agent; pronase; proteinase K; trypsin; unclassified drug; animal cell; antibacterial activity; antiviral activity; article; bacterium isolation; controlled study; cytotoxicity; drug isolation; drug mechanism; drug purification; Enterococcus; enterococcus mundtii; nonhuman; priority journal; soybean; Vero cell; virus inactivation; Animals; Anti-Bacterial Agents; Antiviral Agents; Bacterial Proteins; Cercopithecus aethiops; Enterococcus; Gram-Negative Bacteria; Gram-Positive Bacteria; Microbial Sensitivity Tests; Peptide Hydrolases; Soybeans; Vero Cells; Virus Inactivation; Viruses
Año:2005
Volumen:25
Número:6
Página de inicio:508
Página de fin:513
DOI: http://dx.doi.org/10.1016/j.ijantimicag.2005.02.005
Título revista:International Journal of Antimicrobial Agents
Título revista abreviado:Int. J. Antimicrob. Agents
ISSN:09248579
CODEN:IAAGE
CAS:amylase, 9000-90-2, 9000-92-4, 9001-19-8; pepsin A, 9001-75-6; pronase, 9036-06-0; proteinase K, 39450-01-6; trypsin, 9002-07-7; Anti-Bacterial Agents; Antiviral Agents; Bacterial Proteins; Peptide Hydrolases, EC 3.4.-
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v25_n6_p508_Todorov

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Citas:

---------- APA ----------
Todorov, S.D., Wachsman, M.B., Knoetze, H., Meincken, M. & Dicks, L.M.T. (2005) . An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans. International Journal of Antimicrobial Agents, 25(6), 508-513.
http://dx.doi.org/10.1016/j.ijantimicag.2005.02.005
---------- CHICAGO ----------
Todorov, S.D., Wachsman, M.B., Knoetze, H., Meincken, M., Dicks, L.M.T. "An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans" . International Journal of Antimicrobial Agents 25, no. 6 (2005) : 508-513.
http://dx.doi.org/10.1016/j.ijantimicag.2005.02.005
---------- MLA ----------
Todorov, S.D., Wachsman, M.B., Knoetze, H., Meincken, M., Dicks, L.M.T. "An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans" . International Journal of Antimicrobial Agents, vol. 25, no. 6, 2005, pp. 508-513.
http://dx.doi.org/10.1016/j.ijantimicag.2005.02.005
---------- VANCOUVER ----------
Todorov, S.D., Wachsman, M.B., Knoetze, H., Meincken, M., Dicks, L.M.T. An antibacterial and antiviral peptide produced by Enterococcus mundtii ST4V isolated from soya beans. Int. J. Antimicrob. Agents. 2005;25(6):508-513.
http://dx.doi.org/10.1016/j.ijantimicag.2005.02.005