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Abstract:

The action of five azo-based compounds against the arenaviruses Junin (JUNV) and Tacaribe (TCRV) was evaluated in vitro by a virus yield inhibition assay in Vero cells and a cell-free virion inactivation assay. The compound 2-azo-(1′-(2′-nitroso)naphthyl)-benzoate (ANNB) was the most effective inhibitor of arenavirus production in Vero cells with EC50 (effective concentration 50%) values in the range 6.5-26.2 μM and without inactivating properties. By contrast, the azodicarbonamide (ADA) was very effective in inactivating both arenaviruses with IC50 (inactivating concentration 50%) values of 7.6 and 5.3 μM against JUNV and TCRV, respectively. The virucidal activity of ADA was time- and temperature-dependent. ANNB had no inhibitory action on virus binding or penetration of target cells and did not affect the synthesis of viral proteins. The most likely event susceptible to ANNB would be the process of intracellular virion assembly. © 2002 Elsevier Science B.V. and International Society of Chemotherapy. All rights reserved.

Registro:

Documento: Artículo
Título:Differential inhibitory action of two azoic compounds against arenaviruses
Autor:García, C.C.; Candurra, N.A.; Damonte, E.B.
Filiación:Depto. de Quim. Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Piso 4, Buenos Aires 1428, Argentina
Palabras clave:Antiviral activity; Arenavirus; Azoic compound; Junin virus; Tacaribe virus; Virion inactivation; 2 azo[1' (2' nitroso)naphthyl]benzoate; antivirus agent; azo compound; azoformamide; benzoic acid derivative; chemical compound; unclassified drug; urea derivative; virus protein; Arenavirus; article; chronotherapy; drug activity; drug efficacy; drug inhibition; drug mechanism; drug sensitivity; evaluation; IC 50; in vitro study; inhibition kinetics; nonhuman; priority journal; protein synthesis; target cell; temperature dependence; Vero cell; virion; virus assembly; virus infection
Año:2003
Volumen:21
Número:4
Página de inicio:319
Página de fin:324
DOI: http://dx.doi.org/10.1016/S0924-8579(02)00390-4
Título revista:International Journal of Antimicrobial Agents
Título revista abreviado:Int. J. Antimicrob. Agents
ISSN:09248579
CODEN:IAAGE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v21_n4_p319_Garcia

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Citas:

---------- APA ----------
García, C.C., Candurra, N.A. & Damonte, E.B. (2003) . Differential inhibitory action of two azoic compounds against arenaviruses. International Journal of Antimicrobial Agents, 21(4), 319-324.
http://dx.doi.org/10.1016/S0924-8579(02)00390-4
---------- CHICAGO ----------
García, C.C., Candurra, N.A., Damonte, E.B. "Differential inhibitory action of two azoic compounds against arenaviruses" . International Journal of Antimicrobial Agents 21, no. 4 (2003) : 319-324.
http://dx.doi.org/10.1016/S0924-8579(02)00390-4
---------- MLA ----------
García, C.C., Candurra, N.A., Damonte, E.B. "Differential inhibitory action of two azoic compounds against arenaviruses" . International Journal of Antimicrobial Agents, vol. 21, no. 4, 2003, pp. 319-324.
http://dx.doi.org/10.1016/S0924-8579(02)00390-4
---------- VANCOUVER ----------
García, C.C., Candurra, N.A., Damonte, E.B. Differential inhibitory action of two azoic compounds against arenaviruses. Int. J. Antimicrob. Agents. 2003;21(4):319-324.
http://dx.doi.org/10.1016/S0924-8579(02)00390-4