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Abstract:

Meliacine, a peptide isolated from leaves of Melia azedarach L. inhibited the multiplication of Junin virus in Vero cells treated with the compound before infection (pre-treatment) or immediately after virus adsorption. Analysis of early events following infection demonstrated that meliacine blocks virus penetration by preventing the uncoating step. The addition of meliacine at different times after infection indicated that meliacine also interferes with the release of infectious particles to the extracellular medium and inhibits the low-pH-induced fusion of infected cells. Intracellular transport of viral glycoproteins to the cell membrane was not affected by meliacine, as revealed by immunofluorescence staining. Taken together, these results suggest that meliacine affects two events of the virus replicative cycle that require membrane fusion: uncoating and budding.

Registro:

Documento: Artículo
Título:In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves
Autor:Castilla, V.; Barquero, A.A.; Mersich, S.E.; Coto, C.E.
Filiación:Depto. de Quim. Biológica, Fac. de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Antiviral peptide; Arenaviruses; Junin virus; Meliacine; Membrane fusion; antivirus agent; meliacine; plant extract; unclassified drug; virus glycoprotein; animal cell; antiviral activity; article; cell fusion; concentration response; controlled study; in vitro study; intracellular transport; junin virus; nonhuman; plant leaf; priority journal; vero cell; virus adsorption; Animals; Antiviral Agents; Cercopithecus aethiops; Dose-Response Relationship, Drug; Fluorescent Antibody Technique, Indirect; Giant Cells; Humans; Junin virus; Membrane Fusion; Peptides, Cyclic; Plant Extracts; Plant Leaves; Time Factors; Vero Cells
Año:1998
Volumen:10
Número:1
Página de inicio:67
Página de fin:75
DOI: http://dx.doi.org/10.1016/S0924-8579(98)00011-9
Título revista:International Journal of Antimicrobial Agents
Título revista abreviado:Int. J. Antimicrob. Agents
ISSN:09248579
CODEN:IAAGE
CAS:Antiviral Agents; Peptides, Cyclic; Plant Extracts
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09248579_v10_n1_p67_Castilla

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Citas:

---------- APA ----------
Castilla, V., Barquero, A.A., Mersich, S.E. & Coto, C.E. (1998) . In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves. International Journal of Antimicrobial Agents, 10(1), 67-75.
http://dx.doi.org/10.1016/S0924-8579(98)00011-9
---------- CHICAGO ----------
Castilla, V., Barquero, A.A., Mersich, S.E., Coto, C.E. "In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves" . International Journal of Antimicrobial Agents 10, no. 1 (1998) : 67-75.
http://dx.doi.org/10.1016/S0924-8579(98)00011-9
---------- MLA ----------
Castilla, V., Barquero, A.A., Mersich, S.E., Coto, C.E. "In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves" . International Journal of Antimicrobial Agents, vol. 10, no. 1, 1998, pp. 67-75.
http://dx.doi.org/10.1016/S0924-8579(98)00011-9
---------- VANCOUVER ----------
Castilla, V., Barquero, A.A., Mersich, S.E., Coto, C.E. In vitro anti-Junin virus activity of a peptide isolated from Melia azedarach L. leaves. Int. J. Antimicrob. Agents. 1998;10(1):67-75.
http://dx.doi.org/10.1016/S0924-8579(98)00011-9