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Abstract:

The biological properties mediated by the main envelope glycoprotein (GP1) of a mouse-attenuated mutant of Junin virus, named Cl67, were investigated in comparison with its parental strain XJCl3. In contrast to the parental strain, this mutant was unable to multiply in primary cultures of mouse embryo fibroblasts (MEFs). Impairment of Cl67 multiplication was associated with a lack of virus binding to MEF, probably due to an altered interaction between GP1 and cellular receptors. Antigenic and immunogenic characterization of GP1, performed by neutralization assays, demonstrated that, under certain conditions, polyclonal and monoclonal antibodies exhibited differential affinity and specificity for each virus. Cl67-infected Vero cells showed a marked pH-dependent fusion capability, suggesting more efficient tow pH triggering of fusion by mutant virus GP1 In comparison with the parental strain.

Registro:

Documento: Artículo
Título:Glycoprotein-mediated biological properties of a host range mutant of Junin virus
Autor:Scolaro, L.A.; Damonte, E.B.
Filiación:Laboratorio de Virología, Depto. de Quim. Biol., Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Antigenicity; Attenuation; Binding; Fusion; Glycoprotein; GP1; Host-range mutant; Junin virus; glycoprotein; antigenicity; article; controlled study; Junin virus; nonhuman; priority journal; protein analysis; Vero cell; virus mutant; Junin virus
Año:1997
Volumen:148
Número:5
Página de inicio:323
Página de fin:331
DOI: http://dx.doi.org/10.1016/S0923-2516(97)89128-5
Título revista:Research in Virology
Título revista abreviado:RES. VIROL.
ISSN:09232516
CODEN:RESVE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232516_v148_n5_p323_Scolaro

Referencias:

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Citas:

---------- APA ----------
Scolaro, L.A. & Damonte, E.B. (1997) . Glycoprotein-mediated biological properties of a host range mutant of Junin virus. Research in Virology, 148(5), 323-331.
http://dx.doi.org/10.1016/S0923-2516(97)89128-5
---------- CHICAGO ----------
Scolaro, L.A., Damonte, E.B. "Glycoprotein-mediated biological properties of a host range mutant of Junin virus" . Research in Virology 148, no. 5 (1997) : 323-331.
http://dx.doi.org/10.1016/S0923-2516(97)89128-5
---------- MLA ----------
Scolaro, L.A., Damonte, E.B. "Glycoprotein-mediated biological properties of a host range mutant of Junin virus" . Research in Virology, vol. 148, no. 5, 1997, pp. 323-331.
http://dx.doi.org/10.1016/S0923-2516(97)89128-5
---------- VANCOUVER ----------
Scolaro, L.A., Damonte, E.B. Glycoprotein-mediated biological properties of a host range mutant of Junin virus. RES. VIROL. 1997;148(5):323-331.
http://dx.doi.org/10.1016/S0923-2516(97)89128-5