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Abstract:

The growth characteristics, cytopathogenicity and viral polypeptides of the virulent strain XJ of Junin virus (JV), its attenuated derivative XJC13 and another naturally attenuated JV strain, IV4454, were comparatively studied. IV4454 and XJC13 viruses showed the highest and lowest cytopathology for Vero cells, respectively, as measured by plaque morphology, cell viability and inhibition of host cell protein synthesis. The kinetics and electrophoretic patterns of viral polypeptides in infected cell extracts were very similar among the three strains, whereas differences were detected in the surface glycoprotein GP38 by peptide mapping after limited proteolysis. © 1990.

Registro:

Documento: Artículo
Título:A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides
Autor:Candurra, N.A.; Scolaro, L.A.; Mersich, S.E.; Damonte, E.B.; Coto, C.E.
Filiación:Laboratorio de Virología, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellon 2, Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:Arenaviridae; Attenuated virus; Cytopathogenicity; Junin virus; Polypeptide; Strains; Virulence; virus protein; article; cytotoxicity; human cell; junin virus; nonhuman; priority journal; virogenesis; virus attenuation; virus pathogenesis; virus strain; virus virulence; Animal; Arenaviruses, New World; Cell Line; Comparative Study; Cytopathogenic Effect, Viral; Peptide Mapping; Plaque Assay; Proteins; Support, Non-U.S. Gov't; Vero Cells; Viral Proteins; Arenaviridae; Junin virus
Año:1990
Volumen:141
Número:5
Página de inicio:505
Página de fin:515
DOI: http://dx.doi.org/10.1016/0923-2516(90)90083-U
Título revista:Research in Virology
Título revista abreviado:Res. Virol.
ISSN:09232516
CODEN:RESVE
CAS:Proteins; Viral Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232516_v141_n5_p505_Candurra

Referencias:

  • Bonner, Laskey, A film detection method for tritium-labelled proteins and nucleics acids in polyacrylamide gels (1974) Europ. J. Biochem., 46, pp. 83-88
  • Candurra, Damonte, Coto, Antigenic relationships among attenuated and pathogenic strains of Junin virus (1989) J. med. Virol., 27, pp. 145-150
  • Cleveland, Fischer, Kirschner, Laemmli, Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis (1977) J. biol. Chem., 252, pp. 1103-1106
  • Cresta, Padula, Martinez Segolia, Biological properties of Junin virus proteins. — I. Identification of the immunogenic glycoprotein (1980) Intervirology, 13, pp. 284-288
  • Damonte, Mersich, Candurra, Coto, Cross-reactivity between Junin and Tacaribe viruses as determined by neutralization test and immunoprecipitation (1986) Med. Microbiol. Immunol., 175, pp. 85-88
  • De Mitri, Martinez Segovia, Polypeptide synthesis in Junin-virus-infected BHK-21 cells (1985) Acta Virol., 29, pp. 97-103
  • Dutko, Oldstone, Genomic and biologic variation among commonly used lymphocytic choriomeningitis virus strains (1983) J. gen. Virol., 64, pp. 1689-1698
  • Gonzalez, McCormick, Kiley, Genetic variation among Lassa and Lassa-related arenaviruses analysed by T1-oligonucleotide mapping (1988) Ann. Inst. Pasteur/Virol., 139, pp. 405-420
  • Guerrero, de Weissenbacher, Parodi, Immunización contra la fiebre hemorrágica argentina con una cepa atenuada de virus Junín (1969) Medicina (Buenos Aires), 29, pp. 1-5
  • Howard, Physicochemical properties and chemical composition (1986) Perspectives in medical virology, 2, pp. 23-46. , A.J. Zuckerman, Elsevier Science Publ, Amsterdam
  • Ito, Microassay for studying anticellular effects of human interferon (1984) J. Interferon Res., 4, pp. 603-608
  • Jahrling, Frame, Smith, Monson, Endemic Lassa fever in Liberia. — III. Characterization of Lassa virus isolates (1985) Trans. roy. Soc. trop. Med. Hyg., 79, pp. 374-379
  • Lopez, Franze-Fernandez, Effect of Tacaribe virus infection on host cell protein and nucleic acid synthesis (1985) J. gen. Virol., 66, pp. 1753-1761
  • Mersich, Castilla, Damonte, Lectin affinity of Junin virus glycoproteins (1988) Ann. Inst. Pasteur/Virol., 139, pp. 277-284
  • Scolaro, Mersich, Damonte, A mouse-attenuated mutant of Junin virus with an altered envelope glycoprotein (1990) Arch. Virol., 111, pp. 257-262
  • Weissenbacher, Laguens, Coto, Argentine hemorrhagic fever (1987) Curr. Top. Microbiol. Immunol., 134, pp. 79-116

Citas:

---------- APA ----------
Candurra, N.A., Scolaro, L.A., Mersich, S.E., Damonte, E.B. & Coto, C.E. (1990) . A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides. Research in Virology, 141(5), 505-515.
http://dx.doi.org/10.1016/0923-2516(90)90083-U
---------- CHICAGO ----------
Candurra, N.A., Scolaro, L.A., Mersich, S.E., Damonte, E.B., Coto, C.E. "A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides" . Research in Virology 141, no. 5 (1990) : 505-515.
http://dx.doi.org/10.1016/0923-2516(90)90083-U
---------- MLA ----------
Candurra, N.A., Scolaro, L.A., Mersich, S.E., Damonte, E.B., Coto, C.E. "A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides" . Research in Virology, vol. 141, no. 5, 1990, pp. 505-515.
http://dx.doi.org/10.1016/0923-2516(90)90083-U
---------- VANCOUVER ----------
Candurra, N.A., Scolaro, L.A., Mersich, S.E., Damonte, E.B., Coto, C.E. A comparison of Junin virus strains: Growth characteristics, cytopathogenicity and viral polypeptides. Res. Virol. 1990;141(5):505-515.
http://dx.doi.org/10.1016/0923-2516(90)90083-U