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Abstract:

The development of bacterial communities in replicate lab-scale-activated sludge reactors degrading a non-ionic surfactant was evaluated by statistical analysis of denaturing gradient gel electrophoresis (DGGE) fingerprints. Four sequential batch reactors were fed with synthetic sewage, two of which received, in addition, 0.01% of nonylphenol ethoxylates (NPE). The dynamic character of bacterial community structure was confirmed by the differences in species composition among replicate reactors. Measurement of similarities between reactors was obtained by pairwise similarity analysis using the Bray Curtis coefficient. The group of NPE-amended reactors exhibited the highest similarity values (Sjk = 0.53 ± 0.03), indicating that the bacterial community structure of NPE-amended reactors was better replicated than control reactors (Sjk = 0.36 ± 0.04). Replicate NPE-amended reactors taken at different times of operation clustered together, whereas analogous relations within the control reactor cluster were not observed. The DGGE pattern of isolates grown in conditioned media prepared with media taken at the end of the aeration cycle grouped separately from other conditioned and synthetic media regardless of the carbon source amendment, suggesting that NPE degradation residuals could have a role in the shaping of the community structure. © 2007 Elsevier Masson SAS. All rights reserved.

Registro:

Documento: Artículo
Título:Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities
Autor:Lozada, M.; Basile, L.; Erijman, L.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular (INGEBI-CONICET), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:Community structure; Denaturing gradient gel electrophoresis; Fingerprinting; Microbial ecology; Nonylphenol ethoxylates; nonionic surfactant; phenol derivative; activated sludge; article; bacterial flora; community structure; denaturing gradient gel electrophoresis; fed batch reactor; nonhuman; priority journal; sequencing batch reactor; statistical analysis; Bacteria; Culture Media, Conditioned; Detergents; Electrophoresis; Ethylene Glycols; Sewage; Bacteria (microorganisms)
Año:2007
Volumen:158
Número:8-9
Página de inicio:712
Página de fin:717
DOI: http://dx.doi.org/10.1016/j.resmic.2007.09.010
Título revista:Research in Microbiology
Título revista abreviado:Res. Microbiol.
ISSN:09232508
CODEN:RMCRE
CAS:Culture Media, Conditioned; Detergents; Ethylene Glycols; Sewage; terics, 27986-36-3
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09232508_v158_n8-9_p712_Lozada

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Citas:

---------- APA ----------
Lozada, M., Basile, L. & Erijman, L. (2007) . Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities. Research in Microbiology, 158(8-9), 712-717.
http://dx.doi.org/10.1016/j.resmic.2007.09.010
---------- CHICAGO ----------
Lozada, M., Basile, L., Erijman, L. "Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities" . Research in Microbiology 158, no. 8-9 (2007) : 712-717.
http://dx.doi.org/10.1016/j.resmic.2007.09.010
---------- MLA ----------
Lozada, M., Basile, L., Erijman, L. "Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities" . Research in Microbiology, vol. 158, no. 8-9, 2007, pp. 712-717.
http://dx.doi.org/10.1016/j.resmic.2007.09.010
---------- VANCOUVER ----------
Lozada, M., Basile, L., Erijman, L. Impact of non-ionic surfactant on the long-term development of lab-scale-activated sludge bacterial communities. Res. Microbiol. 2007;158(8-9):712-717.
http://dx.doi.org/10.1016/j.resmic.2007.09.010