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Abstract:

UV treatment increases the frequency of Tn10 precise excision from different sites of the Escherichia coli chromosome. UV induction of Tn10 excision is not evidenced in a lexA3 (ind-) mutant carrying either a recA+ or a recA730 allele. High levels of RecA synthesized by a recA+ gene not repressible by LexA do not relieve the non-inducibility of Tn10 excision in a lexA3 (ind-) background. This indicates that the expression of an SOS gene different from recA is necessary for the induction of Tn10 excision. In contrast to UV induction of point mutations, this induction does not depend on a functional umuC gene since umuC::Tn5 mutants show increased levels of Tn10 excision from leu, thr or gal after irradiation. MucAB+ plasmid pKM101 which renders cells more UV-mutable for point mutations decreases UV-induced Tn10 excision. These results show that UV-induced Tn10 precise excision requires SOS induction and that it involves a pathway different from point mutagenesis. © 1993.

Registro:

Documento: Artículo
Título:Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli
Autor:Levy, M.S.; Balbinder, E.; Nagel, R.
Filiación:CEFYBO, CONICET, Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Department of Biochemistry, Biophysics and Genetics, Colorado Cancer Center, Denver, CO, United States
Palabras clave:Deletions; lexA, umuC, recA mutations; pKM101; Tn10 precise excision; UV induction; article; dna repair; escherichia coli; excision repair; mutagenesis; mutant; mutation; nonhuman; priority journal; ultraviolet radiation; Bacterial Proteins; DNA Transposable Elements; Escherichia coli; Genes, Bacterial; Mutation; Plasmids; Rec A Protein; SOS Response (Genetics); Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.; Ultraviolet Rays; Escherichia coli
Año:1993
Volumen:293
Número:3
Página de inicio:241
Página de fin:247
DOI: http://dx.doi.org/10.1016/0921-8777(93)90075-R
Título revista:Mutation Research-DNA Repair
Título revista abreviado:Mutat. Res. DNA Repair
ISSN:09218777
CODEN:MDRRE
CAS:Bacterial Proteins; DNA Transposable Elements; lexA protein; Plasmids; Rec A Protein
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09218777_v293_n3_p241_Levy

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Citas:

---------- APA ----------
Levy, M.S., Balbinder, E. & Nagel, R. (1993) . Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli. Mutation Research-DNA Repair, 293(3), 241-247.
http://dx.doi.org/10.1016/0921-8777(93)90075-R
---------- CHICAGO ----------
Levy, M.S., Balbinder, E., Nagel, R. "Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli" . Mutation Research-DNA Repair 293, no. 3 (1993) : 241-247.
http://dx.doi.org/10.1016/0921-8777(93)90075-R
---------- MLA ----------
Levy, M.S., Balbinder, E., Nagel, R. "Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli" . Mutation Research-DNA Repair, vol. 293, no. 3, 1993, pp. 241-247.
http://dx.doi.org/10.1016/0921-8777(93)90075-R
---------- VANCOUVER ----------
Levy, M.S., Balbinder, E., Nagel, R. Effect of mutations in SOS genes on UV-induced precise excision of Tn10 in Escherichia coli. Mutat. Res. DNA Repair. 1993;293(3):241-247.
http://dx.doi.org/10.1016/0921-8777(93)90075-R