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Abstract:

Cichlasoma dimerus is a social cichlid fish capable of growing at high rates under laboratory conditions, but knowledge on somatic growth regulation is still unclear. Growth hormone (GH)/insulin-like growth factor I (IGF-I) axis is the key regulator of somatic growth in vertebrates. Two types of growth hormone receptors have been described in teleost fish, named GH receptor type 1 (GHR1) and type 2 (GHR2). In addition, isoforms of these receptors lacking part of the intracellular region have been described. The aim of this study was to evaluate the somatic growth, liver histology and changes in the GH/IGF-I axis after 4 weeks of food deprivation in C. dimerus. Four-week fasted fish showed reductions in specific growth rates in body weight (p < 0.001) and standard length (p < 0.001). Additionally, the hepatosomatic index (p < 0.001) and hepatocyte area (p < 0.001) decreased in fasted fish, while no changes in glucose levels were detected in plasma. The starvation protocol failed to induce changes in GH mRNA levels in the pituitary and IGF-I mRNA levels in liver. In contrast, IGF-I mRNA levels in muscle decreased in fasted fish (p = 0.002). On the other hand, GHR2 (detected with primer sets designed over the extracellular and intracellular region) was upregulated by starvation both in liver and muscle (p < 0.05), while GHR1 remained unchanged. These results show that a fasting period reduced somatic growth both in length and body weight concomitantly with alterations on liver and muscle GHR2 and muscle IGF-I mRNA expression. © 2014, Springer Science+Business Media Dordrecht.

Registro:

Documento: Artículo
Título:GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus
Autor:Delgadin, T.H.; Pérez Sirkin, D.I.; Di Yorio, M.P.; Arranz, S.E.; Vissio, P.G.
Filiación:Laboratorio de Neuroendocrinología del Crecimiento y la Reproducción, DBBE, FCEN-UBA, Ciudad Universitaria, Buenos Aires, C1428EGA, Argentina
IBBEA-CONICET-UBA, Ciudad Universitaria, Buenos Aires, C1428EGA, Argentina
Instituto de Biología Molecular y Celular de Rosario (IBR-CONICET)–Área Biología, Facultad de Ciencias Bioquímicas y Farmacéuticas, CONICET and Universidad Nacional de Rosario, Ocampo y Esmeralda s/n, Rosario (2000), Provincia de Santa Fe, Argentina
Palabras clave:Cichlid; GHR1; GHR2; Growth hormone; IGF-I; growth hormone; growth hormone receptor; messenger RNA; primer DNA; somatomedin C; analysis of variance; animal; body size; cichlid; DNA sequence; food deprivation; gene expression regulation; genetics; growth, development and aging; liver; metabolism; molecular genetics; nucleotide sequence; physiology; real time polymerase chain reaction; skeletal muscle; Analysis of Variance; Animals; Base Sequence; Body Size; Cichlids; DNA Primers; Food Deprivation; Gene Expression Regulation; Growth Hormone; Insulin-Like Growth Factor I; Liver; Molecular Sequence Data; Muscle, Skeletal; Real-Time Polymerase Chain Reaction; Receptors, Somatotropin; RNA, Messenger; Sequence Analysis, DNA
Año:2014
Volumen:41
Número:1
Página de inicio:51
Página de fin:60
DOI: http://dx.doi.org/10.1007/s10695-014-0005-x
Título revista:Fish Physiology and Biochemistry
Título revista abreviado:Fish Physiol. Biochem.
ISSN:09201742
CODEN:FPBIE
CAS:growth hormone, 36992-73-1, 37267-05-3, 66419-50-9, 9002-72-6; somatomedin C, 67763-96-6; DNA Primers; Growth Hormone; Insulin-Like Growth Factor I; Receptors, Somatotropin; RNA, Messenger
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09201742_v41_n1_p51_Delgadin

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Citas:

---------- APA ----------
Delgadin, T.H., Pérez Sirkin, D.I., Di Yorio, M.P., Arranz, S.E. & Vissio, P.G. (2014) . GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus. Fish Physiology and Biochemistry, 41(1), 51-60.
http://dx.doi.org/10.1007/s10695-014-0005-x
---------- CHICAGO ----------
Delgadin, T.H., Pérez Sirkin, D.I., Di Yorio, M.P., Arranz, S.E., Vissio, P.G. "GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus" . Fish Physiology and Biochemistry 41, no. 1 (2014) : 51-60.
http://dx.doi.org/10.1007/s10695-014-0005-x
---------- MLA ----------
Delgadin, T.H., Pérez Sirkin, D.I., Di Yorio, M.P., Arranz, S.E., Vissio, P.G. "GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus" . Fish Physiology and Biochemistry, vol. 41, no. 1, 2014, pp. 51-60.
http://dx.doi.org/10.1007/s10695-014-0005-x
---------- VANCOUVER ----------
Delgadin, T.H., Pérez Sirkin, D.I., Di Yorio, M.P., Arranz, S.E., Vissio, P.G. GH, IGF-I and GH receptors mRNA expression in response to growth impairment following a food deprivation period in individually housed cichlid fish Cichlasoma dimerus. Fish Physiol. Biochem. 2014;41(1):51-60.
http://dx.doi.org/10.1007/s10695-014-0005-x