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The myotomal fibres of the pectoral fins of white croaker (Micropogonias furnieri) have been studied using histochemical techniques and transmission electron microscopy (TEM). Succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black for lipids and myosin-adenosintriphosphatase (mATPase) pre-incubated at alkaline and acid pHs were used to visualize the contraction velocity. Three zones were determined: superficial (SZ), medium (MZ) and deep (DZ). Staining for SDH, PAS and Sudan Black was positive only in the SZ. The level of alkaline mATPase was the highest in fibres from the DZ, intermediate in the MZ and low in the SZ; at an acid pH, the reverse was obtained. Fibres from the SZ were small with large quantities of subsarcolemmal mitochondria, scarce intermyofibrilar mitochondria and a well-developed sarcoplasmic reticulum; the myofibrils displayed a polygonal distribution along the entire length of the fibre. Fibres in the MZ were larger than those in the SZ, the myofibrils were densely packed, mitochondria prevailed under the sarcolemma and the sarcoplasmic reticulum was not abundant. Fibres from the DZ were the largest, with ribbon-shaped myofibrils and scarce mitochondria. The intercellular space was abundant and nervous endings were frequently observed. © Springer Science+Business Media B.V. 2008.


Documento: Artículo
Título:Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study
Autor:Devincenti, C.V.; Díaz, A.O.; García, A.M.; Goldemberg, A.L.
Filiación:Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), 1033AAJ Buenos Aires, Argentina
Idioma: Inglés
Palabras clave:Muscle histochemistry; Pectoral fibres; Subcarangiform swimming; Teleost; TEM; White croaker; animal; article; cytochemistry; cytology; forelimb; histology; methodology; muscle contraction; Perciformes; physiology; skeletal muscle; staining; transmission electron microscopy; ultrastructure; Animals; Forelimb; Histocytochemistry; Microscopy, Electron, Transmission; Muscle Contraction; Muscle Fibers, Skeletal; Perciformes; Staining and Labeling; Genyonemus lineatus; Micropogonias furnieri; Teleostei
Página de inicio:317
Página de fin:323
Título revista:Fish Physiology and Biochemistry
Título revista abreviado:Fish Physiol. Biochem.


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---------- APA ----------
Devincenti, C.V., Díaz, A.O., García, A.M. & Goldemberg, A.L. (2009) . Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study. Fish Physiology and Biochemistry, 35(3), 317-323.
---------- CHICAGO ----------
Devincenti, C.V., Díaz, A.O., García, A.M., Goldemberg, A.L. "Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study" . Fish Physiology and Biochemistry 35, no. 3 (2009) : 317-323.
---------- MLA ----------
Devincenti, C.V., Díaz, A.O., García, A.M., Goldemberg, A.L. "Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study" . Fish Physiology and Biochemistry, vol. 35, no. 3, 2009, pp. 317-323.
---------- VANCOUVER ----------
Devincenti, C.V., Díaz, A.O., García, A.M., Goldemberg, A.L. Pectoral fins of Micropogonias furnieri: A histochemical and ultrastructural study. Fish Physiol. Biochem. 2009;35(3):317-323.