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Abstract:

The Northwestern Argentina (NWA) highland region is one of the southernmost areas of native maize cultivation. We studied variations of different cytological parameters, such as DNA contents, presence/absence of B chromosomes (Bs), and number and sequence composition of heterochromatic knobs in ten accessions of four maize landraces growing along a broad altitudinal cline in NWA. The aim of this work was to assess variations in cytological parameters and their relationship with the crop altitude of cultivation, in an adaptive context. The A-DNA content of the A chromosome complements showed 40% of difference between the lowest (4.5 pg) and the highest (6.3 pg) 2C value. This variation could be attributed to differences in number and size of heterochromatic knobs. Fluorescent in situ hybridization studies revealed the sequence composition of each knob, with a higher proportion of knobs composed of 180-bp repeats rather than TR-1 repeats, in all accessions. We also found numerical polymorphisms and the highest frequency of Bs reported in maize to this date. These results lead us to propose that the frequencies and doses of Bs are influenced by the landrace genotypical make-up. The Bs might be maintained in higher frequencies in those accessions having lower heterochromatin content, so as to preserve an optimal nucleotype. Furthermore, selective forces acting along the altitudinal gradient might be modulating the cytological parameters studied, as suggested by the significant correlations found among them. © 2017, The Botanical Society of Japan and Springer Japan KK, part of Springer Nature.

Registro:

Documento: Artículo
Título:Are cytological parameters of maize landraces (Zea mays ssp. mays) adapted along an altitudinal cline?
Autor:Fourastié, M.F.; Gottlieb, A.M.; Poggio, L.; González, G.E.
Filiación:Laboratorio de Citogenética y Evolución (LaCyE), Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Ecología, Genética y Evolución de Buenos Aires (IEGEBA), CONICET-Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:B chromosomes; DNA content; FISH; Knobs; Maize landraces; plant DNA; altitude; Argentina; chemistry; environment; fluorescence in situ hybridization; genetic variation; genetics; genotype; heterochromatin; maize; plant chromosome; Altitude; Argentina; Chromosomes, Plant; DNA, Plant; Environment; Genetic Variation; Genotype; Heterochromatin; In Situ Hybridization, Fluorescence; Zea mays
Año:2018
Volumen:131
Número:2
Página de inicio:285
Página de fin:296
DOI: http://dx.doi.org/10.1007/s10265-017-0996-3
Título revista:Journal of Plant Research
Título revista abreviado:J. Plant Res.
ISSN:09189440
CAS:DNA, Plant; Heterochromatin
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09189440_v131_n2_p285_Fourastie

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Citas:

---------- APA ----------
Fourastié, M.F., Gottlieb, A.M., Poggio, L. & González, G.E. (2018) . Are cytological parameters of maize landraces (Zea mays ssp. mays) adapted along an altitudinal cline?. Journal of Plant Research, 131(2), 285-296.
http://dx.doi.org/10.1007/s10265-017-0996-3
---------- CHICAGO ----------
Fourastié, M.F., Gottlieb, A.M., Poggio, L., González, G.E. "Are cytological parameters of maize landraces (Zea mays ssp. mays) adapted along an altitudinal cline?" . Journal of Plant Research 131, no. 2 (2018) : 285-296.
http://dx.doi.org/10.1007/s10265-017-0996-3
---------- MLA ----------
Fourastié, M.F., Gottlieb, A.M., Poggio, L., González, G.E. "Are cytological parameters of maize landraces (Zea mays ssp. mays) adapted along an altitudinal cline?" . Journal of Plant Research, vol. 131, no. 2, 2018, pp. 285-296.
http://dx.doi.org/10.1007/s10265-017-0996-3
---------- VANCOUVER ----------
Fourastié, M.F., Gottlieb, A.M., Poggio, L., González, G.E. Are cytological parameters of maize landraces (Zea mays ssp. mays) adapted along an altitudinal cline?. J. Plant Res. 2018;131(2):285-296.
http://dx.doi.org/10.1007/s10265-017-0996-3