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Abstract:

The effects of different combinations of ciprofloxacin (CIP) and norfloxacin (NOR) against Escherichia coli and Staphylococcus aureus were studied using checkerboard, fractional inhibitory concentration (FIC) and time-kill analysis methods. Results obtained by the checkerboard method showed that the more effectives combinations against Escherichia coli were 0.0009 μg/mL CIP0.0312 μg/mL NOR and 0.0037 μg/mL CIP0.0075 μg/mL NOR with a FIC index of 0.62. For Staphylococcus aureus, the combination of 0.0625 μg/mL CIP0.2500 μg/mL NOR showed a synergistic effect, with a FIC index of 0.50. The results of the time-kill method demonstrated either indifference or additivity of the combinations 0.0009 μg/mL CIP+0.0312 μg/mL NOR, 0.0018 μg/mL CIP+0.0312 μg/mL NOR, 0.0037 μg/mL CIP+0.0075 μg/mL NOR and 0.0037 μg/mL CIP+0.0156 μg/mL NOR at 24 h against E. coli. The combination 0.0037 μg/mL CIP+0.0312 μg/mL NOR showed synergistic activity. All the analyzed combinations evidenced bactericidal effects at 4 h. The combinations 0.0625 μg/mL CIP+0.2500 μg/mL NOR and 0.0625 μg/mL CIP+0.0625 μg/mL NOR showed indifference or additivity against S. aureus. None of them generated bactericidal effect at 4 h. Moreover, this last equimolecular combination (equivalent to 1/4 minimum inhibitory concentration (MIC) CIP+1/16 MIC NOR) generated higher reduction of nitro blue tetrazolium than drugs alone. By another way, combinations not equimolecular of CIP and NOR assayed, generated less levels of reactive oxygen species (ROS) than the components alone. © 2017 The Pharmaceutical Society of Japan.

Registro:

Documento: Artículo
Título:Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus
Autor:Vitorino, G.P.; Becerra, M.C.; Barrera, G.D.; Caira, M.R.; Mazzieri, M.R.
Filiación:Departamento de Farmacia, Universidad Nacional de la Patagonia San Juan Bosco, Ciudad Universitaria, Comodoro Rivadavia, Chubut, 9000, Argentina
Departamento de Farmacia, IMBIV-CONICET, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Ciudad Universitaria, Córdoba, 5000, Argentina
Departamento de Química, Facultad de Ciencias Naturales, Universidad Nacional de la Patagonia San Juan Bosco, Ciudad Universitaria, Comodoro Rivadavia, Chubut, 9000, Argentina
Department of Chemistry, University of Cape Town, Rondebosch, 7701, South Africa
Palabras clave:Ciprofloxacin; Combination; Cooperative behavior; Fluoroquinolone; Norfloxacin; Sinergistic effect; ciprofloxacin; norfloxacin; quinoline derived antiinfective agent; reactive oxygen metabolite; antiinfective agent; quinolone derivative; antibacterial activity; antibiotic sensitivity; Article; controlled study; drug effect; drug efficacy; drug mechanism; drug potentiation; Escherichia coli; fractional inhibitory concentration index; in vitro study; minimum inhibitory concentration; nonhuman; protein blood level; Staphylococcus aureus; drug effects; Escherichia coli; growth, development and aging; microbial sensitivity test; Staphylococcus aureus; Anti-Bacterial Agents; Drug Synergism; Escherichia coli; Fluoroquinolones; Microbial Sensitivity Tests; Staphylococcus aureus
Año:2017
Volumen:40
Número:6
Página de inicio:758
Página de fin:764
DOI: http://dx.doi.org/10.1248/bpb.b16-00616
Título revista:Biological and Pharmaceutical Bulletin
Título revista abreviado:Biol. Pharm. Bull.
ISSN:09186158
CODEN:BPBLE
CAS:ciprofloxacin, 85721-33-1; norfloxacin, 70458-96-7; Anti-Bacterial Agents; Fluoroquinolones
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09186158_v40_n6_p758_Vitorino

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Citas:

---------- APA ----------
Vitorino, G.P., Becerra, M.C., Barrera, G.D., Caira, M.R. & Mazzieri, M.R. (2017) . Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus. Biological and Pharmaceutical Bulletin, 40(6), 758-764.
http://dx.doi.org/10.1248/bpb.b16-00616
---------- CHICAGO ----------
Vitorino, G.P., Becerra, M.C., Barrera, G.D., Caira, M.R., Mazzieri, M.R. "Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus" . Biological and Pharmaceutical Bulletin 40, no. 6 (2017) : 758-764.
http://dx.doi.org/10.1248/bpb.b16-00616
---------- MLA ----------
Vitorino, G.P., Becerra, M.C., Barrera, G.D., Caira, M.R., Mazzieri, M.R. "Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus" . Biological and Pharmaceutical Bulletin, vol. 40, no. 6, 2017, pp. 758-764.
http://dx.doi.org/10.1248/bpb.b16-00616
---------- VANCOUVER ----------
Vitorino, G.P., Becerra, M.C., Barrera, G.D., Caira, M.R., Mazzieri, M.R. Cooperative behavior of fluoroquinolone combinations against Escherichia coli and Staphylococcus aureus. Biol. Pharm. Bull. 2017;40(6):758-764.
http://dx.doi.org/10.1248/bpb.b16-00616