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Alexopoulos, E.; Jares-Erijman, E.A.; Jovin, T.M.; Klement, R.; Machinek, R.; Sheldrick, G.M.; Usón, I. "Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT)" (2005) Acta Crystallographica Section D: Biological Crystallography. 61(4):407-415
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Abstract:

The formation of the complex of 7-amino-actinomycin D with potentially single-stranded DNA has been studied by X-ray crystallography in the solid state, by NMR in solution and by molecular modelling. The crystal structures of the complex with 5′-TTAG[Br5U]T-3′ provide interesting examples of MAD phasing in which the dispersive component of the MAD signal was almost certainly enhanced by radiation damage. The trigonal and orthorhombic crystal modifications both contain antibiotic molecules and DNA strands in the form of a 2:4 complex: in the orthorhombic form there is one such complex in the asymmetric unit, while in the trigonal structure there are four. In both structures the phenoxazone ring of the first drug intercalates between a BrU-G (analogous to T-G) wobble pair and a G-T pair where the T is part of a symmetry-related molecule. The chromophore of the second actinomycin intercalates between the BrU-G and G-BrU wobble pairs of the partially paired third and fourth strands. The base stacking also involves (A*T)*T triplets and Watson-Crick A-T pairs and leads to similar complex three-dimensional networks in both structures, with looping-out of unpaired bases. Although the available NOE constraints of a solution containing the antibiotic and d(TTTAGTTT) strands in the ratio 1:1 are insufficient to determine the structure of the complex from the NMR data alone, they are consistent with the intercalation geometry observed in the crystal structure. Molecular-dynamics (MD) trajectories starting from the 1:2 complexes observed in the crystal showed that although the thymines flanking the d(AGT) core are rather flexible and the G-T pairing is not permanently preserved, both strands remain bound to the actinomycin by strong interactions between it and the guanines between which it is sandwiched. Similar strong binding (hemi-intercalation) of the actinomycin to a single guanine was observed in the MD trajectories of a 1:1 complex. The dominant interaction is between the antibiotic and guanine, but the complexes are stabilized further by promiscuous base-pairing. © 2005 International Union of Crystallography - all rights reserved.

Registro:

Documento: Artículo
Título:Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT)
Autor:Alexopoulos, E.; Jares-Erijman, E.A.; Jovin, T.M.; Klement, R.; Machinek, R.; Sheldrick, G.M.; Usón, I.
Filiación:Department of Structural Chemistry, University of Göttingen, Tammannstrasse 4, 37077 Göttingen, Germany
Departamento de Química Orgánica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Department of Molecular Biology, Max-Planck-Institute for Biophysical Chemistry, 37077 Göttingen, Germany
Institute of Organic and Biomolecular Chemistry, University of Göttingen, Tammannstrasse 2, 37077 Göttingen, Germany
ICREA at Instituto de Biología Molecular de Barcelona (IBMB-CSIC), Jordi Girona 18-26, 08034 Barcelona, Spain
Palabras clave:7 aminodactinomycin; 7-aminoactinomycin D; antineoplastic antibiotic; dactinomycin; drug derivative; oligodeoxyribonucleotide; article; base pairing; chemical structure; chemistry; crystallization; electron spin resonance; nuclear magnetic resonance; X ray crystallography; Antibiotics, Antineoplastic; Base Pairing; Crystallization; Crystallography, X-Ray; Dactinomycin; Electron Spin Resonance Spectroscopy; Models, Molecular; Nuclear Magnetic Resonance, Biomolecular; Oligodeoxyribonucleotides
Año:2005
Volumen:61
Número:4
Página de inicio:407
Página de fin:415
DOI: http://dx.doi.org/10.1107/S090744490500082X
Título revista:Acta Crystallographica Section D: Biological Crystallography
Título revista abreviado:Acta Crystallogr. Sect. D Biol. Crystallogr.
ISSN:09074449
CODEN:ABCRE
CAS:7 aminodactinomycin, 7240-37-1; dactinomycin, 1402-38-6, 1402-58-0, 50-76-0; 7-aminoactinomycin D, 7240-37-1; Antibiotics, Antineoplastic; Dactinomycin, 50-76-0; Oligodeoxyribonucleotides
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_09074449_v61_n4_p407_Alexopoulos

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Citas:

---------- APA ----------
Alexopoulos, E., Jares-Erijman, E.A., Jovin, T.M., Klement, R., Machinek, R., Sheldrick, G.M. & Usón, I. (2005) . Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT). Acta Crystallographica Section D: Biological Crystallography, 61(4), 407-415.
http://dx.doi.org/10.1107/S090744490500082X
---------- CHICAGO ----------
Alexopoulos, E., Jares-Erijman, E.A., Jovin, T.M., Klement, R., Machinek, R., Sheldrick, G.M., et al. "Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT)" . Acta Crystallographica Section D: Biological Crystallography 61, no. 4 (2005) : 407-415.
http://dx.doi.org/10.1107/S090744490500082X
---------- MLA ----------
Alexopoulos, E., Jares-Erijman, E.A., Jovin, T.M., Klement, R., Machinek, R., Sheldrick, G.M., et al. "Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT)" . Acta Crystallographica Section D: Biological Crystallography, vol. 61, no. 4, 2005, pp. 407-415.
http://dx.doi.org/10.1107/S090744490500082X
---------- VANCOUVER ----------
Alexopoulos, E., Jares-Erijman, E.A., Jovin, T.M., Klement, R., Machinek, R., Sheldrick, G.M., et al. Crystal and solution structures of 7-amino-actinomycin D complexes with d(TTAGBrUT), d(TTAGTT) and d(TTTAGTTT). Acta Crystallogr. Sect. D Biol. Crystallogr. 2005;61(4):407-415.
http://dx.doi.org/10.1107/S090744490500082X