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Abstract:

Two phosphoproteins of 53,000 and 63,000 mol. wt detected in partially purified preparations of Mucor rouxii cAMP-dependent protein kinase submitted to phosphorylation conditions with [γ- 32 P]ATP are demonstrated to the the result of the autophosphorylation of its regulatory subunit, according to the following criteria: (1) linearity of phosphate incorporation with enzyme sample; (2) independence of phosphate incorporation on temperature; (3) correlation of the phosphoproteins with enzymatic activity in a DEAE-Sepharose chromatography; (4) specific elution of the phosphorylated proteins from cAMP-agarose; (5) phosphorylation of the purified regulatory subunit. Antibodies specific against Mucor regulatory subunit detected an intact subunit of 72,000 mol. wt in crude extracts. Autophosphorylation of the fungal protein kinase A promotes activation of the holoenzyme by cAMP since: (1) under conditions of partial activations, increase of activity is observed when using the phosphoform of the enzyme; (2) release of free catalytic subunit from cAMP-agarose is enhanced when the holoenzyme is previously phosphorylated. © 1992.

Registro:

Documento: Artículo
Título:Autophosphorylation of Mucor rouxii cAMP-dependent protein kinase and its role in holoenzyme activation
Autor:Rossi, S.; Guthmann, M.; Moreno, S.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina
Palabras clave:Autophosphorylation; cAMP-dependent protein kinase; Mucor rouxii; protein kinase A; cyclic amp dependent protein kinase; article; autophosphorylation; enzyme activation; mucor rouxii; nonhuman; priority journal; Adenosine Triphosphate; Antibodies; Binding Sites; Chromatography, Agarose; Enzyme Activation; Fungi; Immunoblotting; Molecular Weight; Phosphorus Radioisotopes; Phosphorylation; Protein Kinases; Support, Non-U.S. Gov't; Amylomyces rouxii; Mucor
Año:1992
Volumen:4
Número:4
Página de inicio:443
Página de fin:451
DOI: http://dx.doi.org/10.1016/0898-6568(92)90038-A
Título revista:Cellular Signalling
Título revista abreviado:Cell. Signal.
ISSN:08986568
CODEN:CESIE
CAS:Adenosine Triphosphate, 56-65-5; Antibodies; Phosphorus Radioisotopes; Protein Kinases, EC 2.7.1.37
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08986568_v4_n4_p443_Rossi

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Citas:

---------- APA ----------
Rossi, S., Guthmann, M. & Moreno, S. (1992) . Autophosphorylation of Mucor rouxii cAMP-dependent protein kinase and its role in holoenzyme activation. Cellular Signalling, 4(4), 443-451.
http://dx.doi.org/10.1016/0898-6568(92)90038-A
---------- CHICAGO ----------
Rossi, S., Guthmann, M., Moreno, S. "Autophosphorylation of Mucor rouxii cAMP-dependent protein kinase and its role in holoenzyme activation" . Cellular Signalling 4, no. 4 (1992) : 443-451.
http://dx.doi.org/10.1016/0898-6568(92)90038-A
---------- MLA ----------
Rossi, S., Guthmann, M., Moreno, S. "Autophosphorylation of Mucor rouxii cAMP-dependent protein kinase and its role in holoenzyme activation" . Cellular Signalling, vol. 4, no. 4, 1992, pp. 443-451.
http://dx.doi.org/10.1016/0898-6568(92)90038-A
---------- VANCOUVER ----------
Rossi, S., Guthmann, M., Moreno, S. Autophosphorylation of Mucor rouxii cAMP-dependent protein kinase and its role in holoenzyme activation. Cell. Signal. 1992;4(4):443-451.
http://dx.doi.org/10.1016/0898-6568(92)90038-A