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Abstract:

Partial activation of Mucor rouxii cAMP-dependent protein kinase by cAMP was obtained when kemptide was used as substrate, but complete activation was attained with cAMP puls protamine or histone. full activation could not be achieved by increasing kemptide or cAMP concentration. Complete activation by cAMP could be obtained by addition of 10 μM polylysine, 10 μM lysine-riche histone or 0.5 mM spermine plus spermidine. The degree of stimulation could be up to 5-fold, depending on the amount of enzyme in the assay. The same concentrations of polycations increased 1.5-2.3 fold the Vmax of kemptide phosphorylation by the ree catalytic subunits of both Mucor and bovine heart protein kinase; 10 μM polyarginine inhibited completely and activity of both enyzmes. © 1990.

Registro:

Documento: Artículo
Título:Polyamines and basic proteins stimulate activation by cAMP and catalytic activity Mucor rouxii
Autor:Guthmann, M.; Pastori, R.; Moreno, S.
Filiación:Departametno de Química Biológica, Facultad de Ciencia Exactas y Naturales, Ciudad Universitaria-Pebellon 2-Piso 4, 1428 Buenos Aires, Argentina
Palabras clave:cAMP-dependent; Mucor rouxii; polyamines; polycations; Protein kinase; cyclic amp; polyamine; protein kinase; animal cell; article; enzyme activation; fungus; nonhuman; priority journal; Animal; Cattle; Cyclic AMP; Enzyme Activation; In Vitro; Kinetics; Mucor; Myocardium; Polyamines; Protein Kinases; Proteins; Substrate Specificity; Support, Non-U.S. Gov't; Amylomyces rouxii; Animalia; Bovinae; Fungi; Mucor
Año:1990
Volumen:2
Número:4
Página de inicio:395
Página de fin:402
DOI: http://dx.doi.org/10.1016/0898-6568(90)90070-Q
Título revista:Cellular Signalling
Título revista abreviado:Cell. Signal.
ISSN:08986568
CODEN:CESIE
CAS:Cyclic AMP, 60-92-4; Polyamines; Protein Kinases, EC 2.7.1.37; Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08986568_v2_n4_p395_Guthmann

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Citas:

---------- APA ----------
Guthmann, M., Pastori, R. & Moreno, S. (1990) . Polyamines and basic proteins stimulate activation by cAMP and catalytic activity Mucor rouxii. Cellular Signalling, 2(4), 395-402.
http://dx.doi.org/10.1016/0898-6568(90)90070-Q
---------- CHICAGO ----------
Guthmann, M., Pastori, R., Moreno, S. "Polyamines and basic proteins stimulate activation by cAMP and catalytic activity Mucor rouxii" . Cellular Signalling 2, no. 4 (1990) : 395-402.
http://dx.doi.org/10.1016/0898-6568(90)90070-Q
---------- MLA ----------
Guthmann, M., Pastori, R., Moreno, S. "Polyamines and basic proteins stimulate activation by cAMP and catalytic activity Mucor rouxii" . Cellular Signalling, vol. 2, no. 4, 1990, pp. 395-402.
http://dx.doi.org/10.1016/0898-6568(90)90070-Q
---------- VANCOUVER ----------
Guthmann, M., Pastori, R., Moreno, S. Polyamines and basic proteins stimulate activation by cAMP and catalytic activity Mucor rouxii. Cell. Signal. 1990;2(4):395-402.
http://dx.doi.org/10.1016/0898-6568(90)90070-Q