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Abstract:

The encapsulation of living cells in materials with good optical and mechanical properties often produces death or stress due to the release of toxic byproducts originated during the synthesis. We present here a method to assess the cellular stress that silica entrapment exerts over living cells taking into account the main preparation variables such as the nature of the silica source, protecting functional groups, total solid concentration, or indirect procedures. Measurement of the cellular stress status of genetically modified Sacharomyces cerevisiae, a true biological probe, allowed us to perform a quantitative analysis of cellular stress in a short time basis (compared to conventional long-term viability tests), opening the gate for a more sophisticated approach to optimize the synthesis conditions. In addition, the aforementioned findings allowed the preparation of novel materials with enhanced optical and mechanical properties. The relation of cellular stress with initial viability is also discussed. © 2008 American Chemical Society.

Registro:

Documento: Artículo
Título:Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress
Autor:Perullini, M.; Jobbágy, M.; Moretti, M.B.; García, S.C.; Bilmes, S.A.
Filiación:INQUIMAE-DQIAQF, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Universitaria, Pab. II, C1428EHA Buenos Aires, Argentina
Palabras clave:Cell growth; Functional groups; Mechanical properties; Optimization; Toxic materials; Cellular stress; Living cells; Optimizing silica encapsulation; Toxic byproducts; Silica
Año:2008
Volumen:20
Número:9
Página de inicio:3015
Página de fin:3021
DOI: http://dx.doi.org/10.1021/cm703075b
Título revista:Chemistry of Materials
Título revista abreviado:Chem. Mater.
ISSN:08974756
CODEN:CMATE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08974756_v20_n9_p3015_Perullini

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Citas:

---------- APA ----------
Perullini, M., Jobbágy, M., Moretti, M.B., García, S.C. & Bilmes, S.A. (2008) . Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress. Chemistry of Materials, 20(9), 3015-3021.
http://dx.doi.org/10.1021/cm703075b
---------- CHICAGO ----------
Perullini, M., Jobbágy, M., Moretti, M.B., García, S.C., Bilmes, S.A. "Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress" . Chemistry of Materials 20, no. 9 (2008) : 3015-3021.
http://dx.doi.org/10.1021/cm703075b
---------- MLA ----------
Perullini, M., Jobbágy, M., Moretti, M.B., García, S.C., Bilmes, S.A. "Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress" . Chemistry of Materials, vol. 20, no. 9, 2008, pp. 3015-3021.
http://dx.doi.org/10.1021/cm703075b
---------- VANCOUVER ----------
Perullini, M., Jobbágy, M., Moretti, M.B., García, S.C., Bilmes, S.A. Optimizing silica encapsulation of living cells: In situ evaluation of cellular stress. Chem. Mater. 2008;20(9):3015-3021.
http://dx.doi.org/10.1021/cm703075b