Artículo
Abstract:
Two cyclic-nucleotide independent soluble casein kinase activities (CK I and CK II) from the fungus Mucor rouxii have been isolated, characterized and found to fit in the general classification of type 1 (CK I) and 2 (CK II) casein kinases, according to their enzymatic and structural properties. Both enzymes phosphorylate acidic substrates, require Mg2+ and have a chromatographic behaviour on DEAE-Sepharose and phosphocellulose similar to their mammalian counterparts. CK I has a sedimentation coefficient of 3.5 S, uses ATP as a phosphate donor (K(m) = 40 μM), phosphorylates casein mainly on serine residues, its activity is strongly inhibited by KCl and polyamines. CK II has a sedimentation coefficient of 7.4 S, uses ATP and GTP as phosphate donors (K(m) ATP = 10 μM; K(m) GTP = 40 μM), phosphorylates casein in serine and threonine, its activity is stimulated by KCl and by polyamines and is inhibited by heparin (I50 = 0.5 μg/ml). Casein kinase activity associated to particulate fraction (40% of total) has been partially characterized and shown to be similar to the soluble CK I activity.
Registro:
| Documento: | Artículo |
| Título: | Characterization of two casein kinase activities in the fungus Mucor rouxii |
| Autor: | Pardo, P.; Moreno, S. |
| Filiación: | Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 1428 Buenos Aires, Argentina |
| Palabras clave: | casein kinase; radioisotope; serine; threonine; fungus; mucor rouxii; nonhuman; priority journal; Amino Acids; Centrifugation, Density Gradient; Chromatography, Affinity; Chromatography, Gel; Comparative Study; Heparin; Kinetics; Mucor; Phosphorylation; Polyamines; Protein Kinases; Solubility; Substrate Specificity; Support, Non-U.S. Gov't |
| Año: | 1988 |
| Volumen: | 12 |
| Número: | 4 |
| Página de inicio: | 183 |
| Página de fin: | 196 |
| Título revista: | Second Messengers and Phosphoproteins |
| Título revista abreviado: | SECOND MESSENGERS PHOSPHOPROTEINS |
| ISSN: | 08957479 |
| CODEN: | SMEPE |
| CAS: | casein kinase, 52660-18-1; serine, 56-45-1, 6898-95-9; threonine, 36676-50-3, 72-19-5; Amino Acids; casein kinase, EC 2.7.1.-; Heparin, 9005-49-6; Polyamines; Protein Kinases, EC 2.7.1.37 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo |
Citas:
---------- APA ----------
Pardo, P. & Moreno, S. (1988) . Characterization of two casein kinase activities in the fungus Mucor rouxii. Second Messengers and Phosphoproteins, 12(4), 183-196.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo [ ]
---------- CHICAGO ----------
Pardo, P., Moreno, S. "Characterization of two casein kinase activities in the fungus Mucor rouxii" . Second Messengers and Phosphoproteins 12, no. 4 (1988) : 183-196.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo [ ]
---------- MLA ----------
Pardo, P., Moreno, S. "Characterization of two casein kinase activities in the fungus Mucor rouxii" . Second Messengers and Phosphoproteins, vol. 12, no. 4, 1988, pp. 183-196.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo [ ]
---------- VANCOUVER ----------
Pardo, P., Moreno, S. Characterization of two casein kinase activities in the fungus Mucor rouxii. SECOND MESSENGERS PHOSPHOPROTEINS. 1988;12(4):183-196.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08957479_v12_n4_p183_Pardo [ ]
