Artículo

Quelas, J.I.; Mongiardini, E.J.; Casabuono, A.; López-García, S.L.; Althabegoiti, M.J.; Covelli, J.M.; Pérez-Giménez, J.; Couto, A.; Lodeiro, A.R. "Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean" (2010) Molecular Plant-Microbe Interactions. 23(12):1592-1604
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Abstract:

Exopolysaccharide (EPS) and lipopolysaccharide (LPS) from Bradyrhizobium japonicum are important for infection and nodulation of soybean (Glycine max), although their roles are not completely understood. To better understand this, we constructed mutants in B. japonicum USDA 110 impaired in galactose or galacturonic acid incorporation into the EPS without affecting the LPS. The derivative LP 3010 had a deletion of lspL-ugdH and produced EPS without galacturonic acid whereas LP 3013, with an insertion in exoB, produced EPS without galactose. In addition, the strain LP 3017, with both mutations, had EPS devoid of both galactosides. The missing galactosides were not replaced by other sugars. The defects in EPS had different consequences. LP 3010 formed biofilms and nodulated but was defective in competitiveness for nodulation; and, inside nodules, the peribacteroid membranes tended to fuse, leading to the merging of symbiosomes. Meanwhile, LP 3013 and LP 3017 were unable to form biofilms and produced empty pseudonodules but exoB suppressor mutants were obtained when LP 3013 plant inoculation was supplemented with wild-type EPS. Similar phenotypes were observed with all these mutants in G. soja. Therefore, the lack of each galactoside in the EPS has a different functional effect on the B. japonicum-soybean symbiosis. © 2010 The American Phytopathological Society.

Registro:

Documento: Artículo
Título:Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean
Autor:Quelas, J.I.; Mongiardini, E.J.; Casabuono, A.; López-García, S.L.; Althabegoiti, M.J.; Covelli, J.M.; Pérez-Giménez, J.; Couto, A.; Lodeiro, A.R.
Filiación:Instituto de Biotecnología y Biología Molecular (IBBM), Departamento de Ciencias Biológicas, Universidad Nacional de la Plata y CCT la Plata-CONICET, Calles 47 y 115 (1900) La Plata, Argentina
Centro de Investigaciones en Hidratos de Carbono (CIHIDECAR), Departamento de Química Orgánica, Universidad de Buenos Aires, Pabellón II, 3er Piso. Ciudad Universitaria, 1428) Buenos Aires, Argentina
Palabras clave:bacterial polysaccharide; bacterial protein; galactose; galacturonic acid; hexuronic acid; article; biofilm; Bradyrhizobium; chemistry; gene expression regulation; genetics; growth, development and aging; metabolism; microbiology; nodulation; physiology; plant root; soybean; symbiosis; ultrastructure; Bacterial Proteins; Biofilms; Bradyrhizobium; Galactose; Gene Expression Regulation, Bacterial; Hexuronic Acids; Plant Root Nodulation; Plant Roots; Polysaccharides, Bacterial; Soybeans; Symbiosis; Bradyrhizobium japonicum; Bradyrhizobium japonicum USDA 110; Glycine max
Año:2010
Volumen:23
Número:12
Página de inicio:1592
Página de fin:1604
DOI: http://dx.doi.org/10.1094/MPMI-05-10-0122
Título revista:Molecular Plant-Microbe Interactions
Título revista abreviado:Mol. Plant-Microbe Interact.
ISSN:08940282
CODEN:MPMIE
CAS:galactose, 26566-61-0, 50855-33-9, 59-23-4; galacturonic acid, 14982-50-4, 685-73-4; Bacterial Proteins; Galactose, 26566-61-0; Hexuronic Acids; Polysaccharides, Bacterial; galacturonic acid, 14982-50-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v23_n12_p1592_Quelas

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Citas:

---------- APA ----------
Quelas, J.I., Mongiardini, E.J., Casabuono, A., López-García, S.L., Althabegoiti, M.J., Covelli, J.M., Pérez-Giménez, J.,..., Lodeiro, A.R. (2010) . Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean. Molecular Plant-Microbe Interactions, 23(12), 1592-1604.
http://dx.doi.org/10.1094/MPMI-05-10-0122
---------- CHICAGO ----------
Quelas, J.I., Mongiardini, E.J., Casabuono, A., López-García, S.L., Althabegoiti, M.J., Covelli, J.M., et al. "Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean" . Molecular Plant-Microbe Interactions 23, no. 12 (2010) : 1592-1604.
http://dx.doi.org/10.1094/MPMI-05-10-0122
---------- MLA ----------
Quelas, J.I., Mongiardini, E.J., Casabuono, A., López-García, S.L., Althabegoiti, M.J., Covelli, J.M., et al. "Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean" . Molecular Plant-Microbe Interactions, vol. 23, no. 12, 2010, pp. 1592-1604.
http://dx.doi.org/10.1094/MPMI-05-10-0122
---------- VANCOUVER ----------
Quelas, J.I., Mongiardini, E.J., Casabuono, A., López-García, S.L., Althabegoiti, M.J., Covelli, J.M., et al. Lack of galactose or galacturonic acid in Bradyrhizobium japonicum USDA 110 exopolysaccharide leads to different symbiotic responses in soybean. Mol. Plant-Microbe Interact. 2010;23(12):1592-1604.
http://dx.doi.org/10.1094/MPMI-05-10-0122