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The role of Mesorhizobium loti surface polysaccharides on the nodulation process is not yet fully understood. In this article, we describe the nodulation phenotype of mutants affected in the synthesis of lipopolysaccharide (LPS) and β(1,2) cyclic glucan. M. loti lpsβ2 mutant produces LPS with reduced amount of O-antigen, whereas M. loti lpsβ1 mutant produces LPS totally devoid of O-antigen. Both genes are clustered in the chromosome. Based on amino acid sequence homology, LPS sugar composition, and enzymatic activity, we concluded that lpsβ2 codes for an enzyme involved in the transformation of dTDP-glucose into dTDP-rhamnose, the sugar donor of rhamnose for the synthesis of O-antigen. On the other hand, Ipsβ1 codes for a glucosyltransferase involved in the biosynthesis of the O-antigen. Although LPS mutants elicited normal nodules, both show reduced competitiveness compared with the wild type. M. loti β(1-2) cyclic glucan synthase (cgs) mutant induces white, empty, ineffective pseudonodules in Lotus tenuis. Cgs mutant induces normal root hair curling but is unable to induce the formation of infection threads. M. loti cgs mutant was more sensitive to deoxycholate and displayed motility impairment compared with the wild-type strain. This pleiotropic effect depends on calcium concentration and temperature. © 2005 The American Phytopathological Society.


Documento: Artículo
Título:Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis
Autor:D'Antuono, A.L.; Casabuono, A.; Couto, A.; Ugalde, R.A.; Lepek, V.C.
Filiación:Inst. de Invest. Biotecnologicas, INTECH, CONICET, Buenos Aires, Argentina
CIHIDECAR, Depto. de Quím. Orgán., UBA, Argentina
IIB-INTECH-UNSAM, INTI, Edificio 24, C. Av. Gen. Paz 5445 entre C. A., Provincia de Buenos Aires, Argentina
Palabras clave:Epimerase/dehydratase family; Glycosyltransferases; LPS and β(1-2) cyclic glucan mutants; calcium; lipopolysaccharide; article; bacterial gene; culture medium; gene expression regulation; genetics; Lotus; metabolism; microbiology; mutation; phenotype; physiology; plant root; Rhizobiaceae; Calcium; Culture Media; Gene Expression Regulation, Bacterial; Genes, Bacterial; Lipopolysaccharides; Lotus; Mutation; Phenotype; Plant Roots; Rhizobiaceae; Lotus tenuis; Mesorhizobium loti
Página de inicio:446
Página de fin:457
Título revista:Molecular Plant-Microbe Interactions
Título revista abreviado:Mol. Plant-Microbe Interact.
CAS:calcium, 7440-70-2; Calcium, 7440-70-2; Culture Media; Lipopolysaccharides


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---------- APA ----------
D'Antuono, A.L., Casabuono, A., Couto, A., Ugalde, R.A. & Lepek, V.C. (2005) . Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis. Molecular Plant-Microbe Interactions, 18(5), 446-457.
---------- CHICAGO ----------
D'Antuono, A.L., Casabuono, A., Couto, A., Ugalde, R.A., Lepek, V.C. "Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis" . Molecular Plant-Microbe Interactions 18, no. 5 (2005) : 446-457.
---------- MLA ----------
D'Antuono, A.L., Casabuono, A., Couto, A., Ugalde, R.A., Lepek, V.C. "Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis" . Molecular Plant-Microbe Interactions, vol. 18, no. 5, 2005, pp. 446-457.
---------- VANCOUVER ----------
D'Antuono, A.L., Casabuono, A., Couto, A., Ugalde, R.A., Lepek, V.C. Nodule development induced by Mesorhizobium loti mutant strains affected in polysaccharide synthesis. Mol. Plant-Microbe Interact. 2005;18(5):446-457.