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Abstract:

Tobacco plants were transformed with the open reading frame 3 gene from Potato virus X (PVX) coding for the p12 protein. Although the transgenic plants exhibited a normal morphological aspect, microscopic examination revealed extensive alterations in leaf tissue structure. After being challenged with PVX, the transgenic plants showed resistance to PVX infection and formation of specific leaf symptoms consisting of concentric rings encircled by necrotic borders. These novel symptoms were accompanied by biochemical changes normally associated with the hypersensitive response (HR) and were absent in noninfected transgenic plants or in PVX-infected nontransgenic plants. No equivalent virus resistance was observed after inoculation with Tobacco mosaic virus or Potato virus Y, suggesting the presence of a specific resistance mechanism. Despite development of HR-like symptoms, systemic acquired resistance was not induced in PVX-infected p12 transgenic plants. No evidence of an RNA-mediated resistance mechanism was found.

Registro:

Documento: Artículo
Título:Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus
Autor:Kobayashi, K.; Cabral, S.; Calamante, G.; Maldonado, S.; Mentaberry, A.
Filiación:INGEBI-CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
Palabras clave:Necrotic lesions; Potexvirus; Triple gene block; Virus movement; disease resistance; hypersensitivity reaction; inoculation; leaf tissue; open reading frame; Potato virus X; potato virus Y; systemic acquired resistance; tobacco; Tobacco mosaic virus; transgenic plant; virus protein; virus resistance; Embryophyta; Nicotiana obtusifolia; Nicotiana tabacum; Potato virus X; Potato virus Y; Potexvirus; Solanum tuberosum; Tobacco mosaic virus
Año:2001
Volumen:14
Número:11
Página de inicio:1274
Página de fin:1285
DOI: http://dx.doi.org/10.1094/MPMI.2001.14.11.1274
Título revista:Molecular Plant-Microbe Interactions
Título revista abreviado:Mol. Plant-Microbe Interact.
ISSN:08940282
CODEN:MPMIE
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08940282_v14_n11_p1274_Kobayashi

Referencias:

  • An, G., Ebert, P.R., Mitra, A., Ha, S.M., Binary vectors. Section A3 (1988) Plant Molecular Biology Manual, pp. 1-19. , S. B. Gelvin and R. A. Schilperoort, eds. Kluwer Academic, Dordrecht, the Netherlands
  • Angell, S.M., Davies, C., Baulcombe, D.C., Cell-to-cell movement of potato virus X is associated with a change in the size-exclusion limit of plasmodesmata in trichome cells of Nicotiana clevelandii (1996) Virology, 216, pp. 197-201
  • Ares, X., Calamante, G., Cabral, S., Lodge, J., Hemenway, P., Beachy, R.N., Mentaberry, A., Transgenic plants expressing Potato virus X ORF2 protein (p24) are resistant to Tobacco mosaic virus and Ob tobamoviruses (1998) J. Virol., 72, pp. 731-738
  • Beck, D.L., Guilford, P.J., Voot, D.M., Andersen, M.T., Forster, R.L., Triple gene block proteins of White clover mosaic potexvirus are required for transport (1991) Virology, 183, pp. 695-702
  • Beck, D.L., Van Dolleweerd, C.J., Lough, T.J., Balmori, E., Voot, D.M., Andersen, M.T., O'Brien, I.E., Forster, R.L., Disruption of virus movement confers broad-spectrum resistance against systemic infection by plant viruses with a triple gene block (1994) Proc. Natl. Acad. Sci. U.S.A., 91, pp. 10310-10314
  • Bleykasten-Grosshans, C., Guilley, H., Bouzoubaa, S., Richards, K.E., Jonard, G., Independent expression of the first two triple gene block proteins of beet necrotic yellow vein virus complements virus defective in the corresponding gene but expression of the third protein inhibits viral cell-to-cell movement (1997) Mol. Plant-Microbe Interact., 10, pp. 240-246
  • Bol, J.F., Linthorst, H.J.M., Cornelissen, B.J.C., Plant pathogenesis-related proteins induced by virus infection (1990) Annu. Rev. Phytopathol., 28, pp. 113-138
  • Brederode, F.T., Linthorst, H.J.M., Bol, J.F., Differential induction of acquired resistance and PR gene expression in tobacco by virus infection, ethephon treatment, UV light and wounding (1991) Plant Mol. Biol., 17, pp. 1117-1125
  • Calamante, G., (1998) Utilización del virus X de la papa como vector de expresión transitoria en plantas, , Doctoral thesis. University of Buenos Aires, Buenos Aires
  • Carrington, J.C., Kasschau, K.D., Mahajan, S.K., Schaad, M.C., Cell-to-cell and long-distance transport of viruses in plants (1996) Plant Cell, 8, pp. 1669-1681
  • Chang, B.Y., Lin, N.S., Liou, D.Y., Chen, J.P., Liou, G.G., Hsu, Y.H., Subcellular localization of the 28-kDa protein of the triple-gene-block of Bamboo mosaic potexvirus (1997) J. Gen. Virol., 78, pp. 1175-1179
  • Chapman, S., Kavanagh, T., Baulcombe, D., Potato virus X as a vector for gene expression in plants (1992) Plant J., 2, pp. 549-557
  • Davies, C., Hills, G., Baulcombe, D.C., Sub-cellular localization of the 25-kDa protein encoded in the triple gene block of Potato virus X (1993) Virology, 197, pp. 166-175
  • Deom, C.M., Lapidot, M., Beachy, R.N., Plant virus movement proteins (1992) Cell, 69, pp. 221-224
  • Erhardt, M., Stussi-Garaud, C., Guilley, H., Richards, K.E., Jonard, G., Bouzoubaa, S., The first triple gene block protein of Peanut clump virus localizes to the plasmodesmata during virus infection (1999) Virology, 264, pp. 220-229
  • Forster, R.L.S., Bevan, M.W., Harbison, S., Gardner, R.C., The complete sequence of the White clover mosaic potexvirus (1988) Nucleic Acids Res., 16, pp. 291-304
  • Forster, R.L., Beck, D.L., Guilford, P.J., Voot, D.M., Van Dolleweerd, C.J., Andersen, M.T., The coat protein of White clover mosaic potexvirus has a role in facilitating cell-to-cell transport in plants (1992) Virology, 191, pp. 480-484
  • Fulcher, R.G., Fluorescence microscopy of cereals (1982) Food Microstructure, 1, pp. 167-175
  • Gorbalenya, A.E., Koonin, E.V., Donchenko, A.P., Blinov, V.M., A novel superfamily of nucleoside triphosphate-binding motif containing proteins which are probably involved in duplex unwinding in DNA and RNA replication and recombination (1988) FEBS (Fed. Eur. Biochem. Soc.) Lett., 235, pp. 16-24
  • Hefferon, K.L., Doyle, S., AbouHaidar, M.G., Immunological detection of the 8K protein of Potato virus X (PVX) in cell walls of PVX-infected tobacco and transgenic potato (1997) Arch. Virol., 142, pp. 425-433
  • Kalinina, N.O., Fedorkin, O.N., Samuilova, O.V., Maiss, E., Korpela, T., Morozov, S., Atabekov, J.G., Expression and biochemical analyses of the recombinant Potato virus X 25K movement protein (1996) FEBS (Fed. Eur. Biochem. Soc.) Lett., 397, pp. 75-78
  • Koonin, E.V., Dolja, V.V., Evolution and taxonomy of positive-strand RNA viruses: Implications of comparative analysis of amino acid sequences (1993) Crit. Rev. Biochem. Mol. Biol., 28, pp. 375-430
  • Lough, T.J., Shash, K., Xoconostle-Cázares, B., Hofstra, K.R., Beck, D.L., Balmori, E., Forster, R.L.S., Lucas, W.J., Molecular dissection of the mechanism by which potexvirus triple gene block protein mediate cell-to-cell transport of infectious RNA (1998) Mol. Plant-Microbe Interact., 11, pp. 801-814
  • Lough, T.J., Netzler, N.E., Emerson, S.J., Sutherland, P., Carr, F., Beck, D.L., Lucas, W.J., Forster, R.L.S., Cell-to-cell movement of potexviruses: Evidence for a ribonucleoprotein complex involving the coat protein and first triple gene block protein (2000) Mol. Plant-Microbe Interact., 13, pp. 962-974
  • Lucas, W.J., Wolf, S., Connections between virus movement, macromolecular signaling and assimilate allocation (1999) Curr. Opin. Plant Biol., 2, pp. 192-197
  • Malcuit, I., Marano, M.R., Kavanagh, T.A., DeJong, W., Forsynth, A., Baulcombe, D.C., The 25-kDa movement protein of PVX elicits Nb-mediated hypersensitive cell death in potato (1999) Mol. Plant-Microbe Interact., 12, pp. 536-543
  • Meshi, T., Motoyoshi, F., Maeda, T., Yoshiwoka, S., Watanabe, H., Okada, Y., Mutations in the Tobacco mosaic virus 30-kD protein gene overcome Tm-2 resistance in tomato (1989) Plant Cell, 1, pp. 515-522
  • Morozov, S., Lukasheva, L.I., Chemov, B.K., Skryabin, K.G., Atabekov, J.G., Nucleotide sequence of the open reading frames adjacent of the coat protein cistron in Potato virus X genome (1987) FEBS (Fed. Eur. Biochem. Soc.) Lett., 213, pp. 438-442
  • Morozov, S., Dolja, V.V., Atabekov, J.G., Probable reassortment of genomic elements among elongated RNA-containing plant viruses (1989) J. Mol. Evol., 29, pp. 52-62
  • Morozov, S.Y., Miroshnichenko, N.A., Zelenina, D.A., Fedorkin, O.N., Solovijev, A.G., Lukasheva, L.I., Atabekov, J.C., Expression of RNA transcripts of Potato virus X full-length and subgenomic cDNAs (1990) Biochimie, 72, pp. 677-684
  • Morozov, S., Miroshnichenko, N.A., Solovyev, A.G., Fedorkin, O.N., Zelenina, D.A., Lukasheva, L.I., Karasev, A.V., Atabekov, J.G., Expression strategy of the Potato virus X triple gene block (1991) J. Gen. Virol., 72, pp. 2039-2042
  • Morozov, S., Miroshnichenko, N.A., Solovyev, A.G., Zelenina, D.A., Fedorkin, O.N., Lukasheva, L.I., Grachev, S.A., Chernov, B.K., In vitro membrane binding of the translation products of the Carlavirus 7 kDa protein genes (1991) Virology, 183, pp. 782-785
  • Morozov, S., Fedorkin, O.N., Juttner, G., Schiemann, J., Baulcombe, D.C., Atabekov, J.G., Complementation of a Potato virus X mutant mediated by bombardment of plant tissues with cloned viral movement protein genes (1997) J. Gen. Virol., 78, pp. 2077-2083
  • Mushegian, A.R., Koonin, E.V., Cell-to-cell movement of plant viruses. Insights from amino acid sequence comparisons of movement proteins and from analogies with cellular transport systems (1993) Arch. Virol., 133, pp. 239-257
  • O'Brien, T.P., McCully, M.E., (1981) The Study of Plant Structure: Principles and Selected Methods, , Thermocarphy Pty. Lds., Melbourne, Australia
  • Oparka, K.J., Roberts, A.G., Roberts, I.M., Prior, D.A., Santa Cruz, S., Viral coat protein is targeted to, but does not gate, plasmodesmata during cell-to-cell movement of Potato virus X (1996) Plant J., 10, pp. 805-813
  • Orman, B.E., Celnik, R.M., Mandel, A.M., Torres, H.N., Mentaberry, A.N., Complete cDNA sequence of a South American isolate of Potato virus X (1990) Virus Res., 16, pp. 293-305
  • Padgett, H.S., Beachy, R.N., Analysis of a Tobacco mosaic virus strain capable of overcoming N gene-mediated resistance (1993) Plant Cell, 5, pp. 577-586
  • Ponz, F., Bruening, G., Mechanisms of resistance to plant viruses (1986) Annu. Rev. Phytopathol., 24, pp. 355-383
  • Ross, A.F., Systemic acquired resistance induced by localized virus infections in plants (1961) Virology, 14, pp. 340-358
  • Rouleau, M., Smith, R.J., Bancroft, J.B., Mackie, G.A., Purification, properties and subcellular localization of Foxtail mosaic potexvirus 26-kDa protein (1994) Virology, 204, pp. 254-265
  • Ruiz, M.T., Voinnet, O., Baulcombe, D.C., Initiation and maintenance of virus-induced gene silencing (1998) Plant Cell, 10, pp. 937-946
  • Ryals, J.A., Neuenschwander, U.H., Willits, M.G., Molina, A., Steiner, H.Y., Hunt, M.D., Systemic acquired resistance (1996) Plant Cell, 8, pp. 1809-1819
  • Santa Cruz, S., Roberts, A.G., Prior, D.A., Chapman, S., Oparka, K.J., Cell-to-cell and phloem-mediated transport of Potato virus X. The role of virions (1998) Plant Cell, 10, pp. 495-510
  • Seppanen, P., Puska, R., Honkanen, J., Tyulkina, L.G., Fedorkin, O., Morozov, S., Atabekov, J.G., Movement protein-derived resistance to triple gene block-containing plant viruses (1997) J. Gen. Virol., 78, pp. 1241-1246
  • Skryabin, K.G., Morozov, S., Kraev, A.S., Rozanov, M.N., Chernov, B.K., Lukasheva, L.I., Atabekov, J.G., Conserved and variable elements in RNA genomes of Potexviruses (1988) FEBS (Fed. Eur. Biochem. Soc.) Lett., 240, pp. 33-40
  • Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Gartner, F.H., Provenzano, M.D., Fujimoto, E.K., Klenck, D.C., Measurement of protein using bicinchoninic acid (1985) Anal. Biochem., 150, pp. 76-85
  • Solovyev, A.G., Stroganova, T.A., Zamiatnin A.A., Jr., Fedorkin, O.N., Schiemann, J., Morozov, S.Y., Subcellular sorting of small membrane-associated triple gene block proteins: TGBp3-assisted targeting of TGBp2 (2000) Virology, 269, pp. 113-127
  • Verchot, J., Angell, S.M., Baulcombe, D.C., In vivo translation of the triple gene block of Potato virus X requires two subgenomic mRNAs (1998) J. Virol., 72, pp. 8316-8320
  • Verwoerd, T., Dekekr, B., Hoekema, A., A small-scale procedure for the rapid isolation of plant RNAs (1989) Nucleic Acids Res., 17, p. 2362
  • Weber, H., Pfitzner, A.J.P., Tm-22 resistance in tomato requires recognition of the carboxy terminus of the movement protein of tomato mosaic virus (1998) Mol. Plant-Microbe Interact., 11, pp. 498-503
  • Yang, Y., Ding, B., Baulcombe, D.C., Verchot, J., Cell-to-cell movement of the 25K protein of Potato virus X is regulated by three other viral proteins (2000) Mol. Plant-Microbe Interact., 13, pp. 599-605
  • Zhou, H., Jackson, A.O., Expression of the Barley stripe mosaic virus RNA β "triple gene block" (1996) Virology, 216, pp. 364-379

Citas:

---------- APA ----------
Kobayashi, K., Cabral, S., Calamante, G., Maldonado, S. & Mentaberry, A. (2001) . Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus. Molecular Plant-Microbe Interactions, 14(11), 1274-1285.
http://dx.doi.org/10.1094/MPMI.2001.14.11.1274
---------- CHICAGO ----------
Kobayashi, K., Cabral, S., Calamante, G., Maldonado, S., Mentaberry, A. "Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus" . Molecular Plant-Microbe Interactions 14, no. 11 (2001) : 1274-1285.
http://dx.doi.org/10.1094/MPMI.2001.14.11.1274
---------- MLA ----------
Kobayashi, K., Cabral, S., Calamante, G., Maldonado, S., Mentaberry, A. "Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus" . Molecular Plant-Microbe Interactions, vol. 14, no. 11, 2001, pp. 1274-1285.
http://dx.doi.org/10.1094/MPMI.2001.14.11.1274
---------- VANCOUVER ----------
Kobayashi, K., Cabral, S., Calamante, G., Maldonado, S., Mentaberry, A. Transgenic tobacco plants expressing the Potato virus X open reading frame 3 gene develop specific resistance and necrotic ring symptoms after infection with the homologous virus. Mol. Plant-Microbe Interact. 2001;14(11):1274-1285.
http://dx.doi.org/10.1094/MPMI.2001.14.11.1274