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A highly heterogeneous cell line, IIB‐MEL‐J, was established from a human metastatic melanoma. This cell line contains small cells, dendritic cells, and megacells with multiple nuclei. IIB‐MEL‐J expresses S 100, cytokeratin intermediate filaments and the gangliosides GD2 and GD3. It requires growth factors (insulin, EGF, and transferrin) and antioxidants for optimal growth. When plated under optimal conditions, IIB‐MEL‐J grows with a doubling time of 70–80 hours. The cells may be fractionated by Percoll gradient centrifugation into several subpopulations (A, B, and C) with different characteristics. Subpopulation A is the slowest growing, and most of the DNA‐synthesizing cells are concentrated in fractions B and C. Every subpopulation expresses S 100 and cytokeratin intermediate filaments, whereas only subpopulations B and C express GD2 and GD3. Pigmented cells are concentrated mainly in subpopulation C. Cytogenetic analysis of IIB‐MEL‐J revealed extensive chromosomal alterations, including a highly heterogeneous chromosome number and chromosomal rearrangements, gains, losses, isochromosomes, and double minutes. This highly heterogeneous cell line may be helpful to study cellular differentiation and interaction between different subpopulations in human melanoma. Copyright © 1989, Wiley Blackwell. All rights reserved


Documento: Artículo
Título:Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line
Filiación:Instituto de Investigaciones Bioquimicas "Fundacion Campomar", Facultad de Ciencias Exactas Y Naturales, Universidad National de Buenos Aires, Av. Patricias Argentinas 435, Buenos Aires, 1405, Argentina
Academia Nacional de Medicina, Coronel Diaz 2497, Buenos Aires, 1425, Argentina
Palabras clave:Cellular subpopulations; Cytogenetics; Growth factors; adult; article; case report; cell culture; cell transformation; chromosome; chromosome aberration; chromosome disorder; electron microscopy; human; male; melanoma; pathology; phenotype; ultrastructure; Case Report; Cell Transformation, Neoplastic; Chromosome Aberrations; Chromosome Disorders; Chromosomes; Human; Male; Melanoma; Microscopy, Electron; Middle Age; Phenotype; Support, Non-U.S. Gov't; Tumor Cells, Cultured
Página de inicio:504
Página de fin:509
Título revista:Pigment Cell Research
Título revista abreviado:Pigm. Cell Res.


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---------- APA ----------
GUERRA, L., MORDOH, J., SLAVUTSKY, I., LARRIPA, I. & MEDRANO, E.E. (1989) . Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line. Pigment Cell Research, 2(6), 504-509.
---------- CHICAGO ----------
GUERRA, L., MORDOH, J., SLAVUTSKY, I., LARRIPA, I., MEDRANO, E.E. "Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line" . Pigment Cell Research 2, no. 6 (1989) : 504-509.
---------- MLA ----------
GUERRA, L., MORDOH, J., SLAVUTSKY, I., LARRIPA, I., MEDRANO, E.E. "Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line" . Pigment Cell Research, vol. 2, no. 6, 1989, pp. 504-509.
---------- VANCOUVER ----------
GUERRA, L., MORDOH, J., SLAVUTSKY, I., LARRIPA, I., MEDRANO, E.E. Characterization of IIB‐MEL‐J: A New and Highly Heterogenous Human Melanoma Cell Line. Pigm. Cell Res. 1989;2(6):504-509.