Abstract:
Angiogenesis, the growth OF new capillaries from preexisting blood vessels, is a complex process involving endothelial cell (EC) activation, disruption of vascular basement membranes, and migration and proliferation of ECs. Glycan-mediated recognition has been proposed to play an instrumental role in mediating cell-cell and cell-matrix interactions. Galectins (Gal), a family of glycan-binding proteins with affinity for β-galactosides and a conserved sequence motif, can decipher glycan-containing information and mediate cell-cell communication. Galectin-8 (Gal-8), a member of this family, is a bivalent "tandem-repeat"-type galectin, which possesses 2 CRDs connected by a linker peptide. Here, we show that Gal-8 is endowed with proangiogeneic properties. Functional assays revealed a critical role for this lectin in the regulation of capillary-tube formation and EC migration. Moreover, Matrigel, either supplemented with Gal-8 or vascular endothelial growth factor (VEGF), injected in mice resulted in induction of in vivo angiogenesis. Remarkably, Gal-8 was expressed both in the cytoplasm and nucleus in ECs of normal and tumor vessels. Furthermore, CD166 [activated leukocyte cell adhesion molecule (ALCAM)] was identified as a specific Gal-8-binding partner in normal vascular ECs. Collectively, these data provide the first evidence demonstrating an essential role for Gal-8 in the regulation of angiogenesis with critical implications in tumor biology. © FASEB.
Registro:
Documento: |
Artículo
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Título: | Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8 |
Autor: | Cárdenas Delgado, V.M.; Nugnes, L.G.; Colombo, L.L.; Troncoso, M.F.; Fernández, M.M.; Malchiodi, E.L.; Frahm, I.; Croci, D.O.; Compagno, D.; Rabinovich, G.A.; Wolfenstein-Todel, C.; Elola, M.T. |
Filiación: | Instituto de Química Y Fisicoquímica Biológicas (UBA-CONICET), Facultad de Farmacia Y Bioquímica, Universidad de Buenos Aires, Junín 956 (C1113), Buenos Aires, Argentina Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni, Universidad de Buenos Aires, Facultad de Farmacia Y Bioquímica, Buenos Aires, Argentina Área de Investigación, Instituto de Oncología A. H. Roffo, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Química Biológica, Facultad de Ciencias Exactas Y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina Departamento de Patología, Sanatorio Mater Dei, Buenos Aires, Argentina Laboratorio de Inmunopatología, Instituto de Biología Y Medicina Experimental (IBYME-CONICET), Buenos Aires, Argentina
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Palabras clave: | Capillary formation; Glycan-binding proteins; Invasion; activated leukocyte cell adhesion molecule; galectin; galectin 1; galectin 3; galectin 8; vasculotropin; angiogenesis; animal cell; animal experiment; article; cell invasion; cell migration; cell nucleus; controlled study; cytoplasm; endothelium cell; female; human; human cell; modulation; mouse; nonhuman; priority journal; protein binding; protein determination; protein expression; tandem repeat; Activated-Leukocyte Cell Adhesion Molecule; Animals; Antigens, CD31; Cell Line; Cell Movement; Cell Nucleus; Collagen; Cytoplasm; Dose-Response Relationship, Drug; Drug Combinations; Endothelial Cells; Female; Galectins; Humans; Immunoblotting; Immunohistochemistry; Immunoprecipitation; Kinetics; Laminin; Mice; Mice, Inbred BALB C; Neoplasms; Neovascularization, Physiologic; Protein Binding; Proteoglycans; Rats; Recombinant Proteins; Vascular Endothelial Growth Factor A; Mus |
Año: | 2011
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Volumen: | 25
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Número: | 1
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Página de inicio: | 242
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Página de fin: | 254
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DOI: |
http://dx.doi.org/10.1096/fj.09-144907 |
Título revista: | FASEB Journal
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Título revista abreviado: | FASEB J.
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ISSN: | 08926638
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CODEN: | FAJOE
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CAS: | galectin 1, 258495-34-0; galectin 3, 208128-56-7; galectin 8, 220452-97-1; vasculotropin, 127464-60-2; Activated-Leukocyte Cell Adhesion Molecule; Antigens, CD31; Collagen, 9007-34-5; Drug Combinations; Galectins; LGALS8 protein, human; Laminin; Proteoglycans; Recombinant Proteins; Vascular Endothelial Growth Factor A; matrigel, 119978-18-6
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v25_n1_p242_CardenasDelgado |
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Citas:
---------- APA ----------
Cárdenas Delgado, V.M., Nugnes, L.G., Colombo, L.L., Troncoso, M.F., Fernández, M.M., Malchiodi, E.L., Frahm, I.,..., Elola, M.T.
(2011)
. Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8. FASEB Journal, 25(1), 242-254.
http://dx.doi.org/10.1096/fj.09-144907---------- CHICAGO ----------
Cárdenas Delgado, V.M., Nugnes, L.G., Colombo, L.L., Troncoso, M.F., Fernández, M.M., Malchiodi, E.L., et al.
"Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8"
. FASEB Journal 25, no. 1
(2011) : 242-254.
http://dx.doi.org/10.1096/fj.09-144907---------- MLA ----------
Cárdenas Delgado, V.M., Nugnes, L.G., Colombo, L.L., Troncoso, M.F., Fernández, M.M., Malchiodi, E.L., et al.
"Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8"
. FASEB Journal, vol. 25, no. 1, 2011, pp. 242-254.
http://dx.doi.org/10.1096/fj.09-144907---------- VANCOUVER ----------
Cárdenas Delgado, V.M., Nugnes, L.G., Colombo, L.L., Troncoso, M.F., Fernández, M.M., Malchiodi, E.L., et al. Modulation of endothelial cell migration and angiogenesis: A novel function for the "tandem-repeat" lectin galectin-8. FASEB J. 2011;25(1):242-254.
http://dx.doi.org/10.1096/fj.09-144907