Pacienza, N.; Pozner, R.G.; Bianco, G.A.; D'Atri, L.P.; Croci, D.O.; Negrotto, S.; Malaver, E.; Gómez, R.M.; Rabinovich, G.A.; Schattner, M. "The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation" (2008) FASEB Journal. 22(4):1113-1123
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Platelet activation is a critical process during inflammation, thrombosis, and cancer. Here, we show that galectin-1, an endogenous lectin with immunoregulatory properties, plays a key role in human platelet activation and function. Galectin-1 binds to human platelets in a carbohydrate-dependent manner and synergizes with ADP or thrombin to induce platelet aggregation and ATP release. Furthermore, galectin-1 induces F-actin polymerization, up-regulation of P-selectin, and GPIIIa expression; promotes shedding of microvesicles; and triggers conformational changes in GPIIb/IIIa. In addition, exposure to this lectin favors the generation of leukocyte-platelet aggregates. A further mechanistic analysis revealed the involvement of Ca2+ and cyclic nucleotide-dependent pathways in galectin-1-mediated control of platelet activation. Finally, expression of endogenous galectin-1 in human platelets contributes to ADP-induced aggregation. Our study reveals a novel unrecognized role for galectin-1 in the control of platelet physiology with potential implications in thrombosis, inflammation, and metastasis. © FASEB.


Documento: Artículo
Título:The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation
Autor:Pacienza, N.; Pozner, R.G.; Bianco, G.A.; D'Atri, L.P.; Croci, D.O.; Negrotto, S.; Malaver, E.; Gómez, R.M.; Rabinovich, G.A.; Schattner, M.
Filiación:Hematological Research Institute, National Academy of Medicine, National Research Council (CONICET), Buenos Aires, Argentina
Laboratory of Immunopathology, Institute of Biology and Experimental Medicine (IBYME), National Research Council (CONICET), Buenos Aires, Argentina
Biochemistry and Molecular Biology Institute, Department of Biological Sciences, National University of La Plata, La Plata, Argentina
Department of Biological Chemistry, Faculty of Exact and Natural Sciences, University of Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Pacheco de Melo 3081, Buenos Aires (1425), Argentina
Instituto de Biologia Y Medicina Experimental, CONICET, Vuelta de Obligado 2490, Buenos Aires (1428), Argentina
Palabras clave:Hemostasis; Inflammation; Lectin; P-selectin; adenosine diphosphate; adenosine triphosphate; calcium ion; carbohydrate binding protein; cyclic nucleotide; F actin; galectin 1; glycan; glycoprotein IIIa; PADGEM protein; thrombin; article; cell interaction; controlled study; human; human cell; immunoregulation; leukocyte; normal human; polymerization; priority journal; protein binding; protein induction; thrombocyte activation; thrombocyte aggregation; thrombocyte function; thrombocyte release reaction; upregulation; Actins; Adenosine Diphosphate; Binding Sites; Blood Platelets; Flow Cytometry; Galectin 1; Humans; Integrin beta3; Leukocytes; Microscopy, Confocal; P-Selectin; Platelet Activation; Platelet Aggregation; Signal Transduction
Página de inicio:1113
Página de fin:1123
Título revista:FASEB Journal
Título revista abreviado:FASEB J.
CAS:adenosine diphosphate, 20398-34-9, 58-64-0; adenosine triphosphate, 15237-44-2, 56-65-5, 987-65-5; calcium ion, 14127-61-8; F actin, 39409-31-9; galectin 1, 258495-34-0; thrombin, 9002-04-4; Actins; Adenosine Diphosphate, 58-64-0; Galectin 1; Integrin beta3; P-Selectin


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---------- APA ----------
Pacienza, N., Pozner, R.G., Bianco, G.A., D'Atri, L.P., Croci, D.O., Negrotto, S., Malaver, E.,..., Schattner, M. (2008) . The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation. FASEB Journal, 22(4), 1113-1123.
---------- CHICAGO ----------
Pacienza, N., Pozner, R.G., Bianco, G.A., D'Atri, L.P., Croci, D.O., Negrotto, S., et al. "The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation" . FASEB Journal 22, no. 4 (2008) : 1113-1123.
---------- MLA ----------
Pacienza, N., Pozner, R.G., Bianco, G.A., D'Atri, L.P., Croci, D.O., Negrotto, S., et al. "The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation" . FASEB Journal, vol. 22, no. 4, 2008, pp. 1113-1123.
---------- VANCOUVER ----------
Pacienza, N., Pozner, R.G., Bianco, G.A., D'Atri, L.P., Croci, D.O., Negrotto, S., et al. The immunoregulatory glycan-binding protein galectin-1 triggers human platelet activation. FASEB J. 2008;22(4):1113-1123.