Artículo

Masuda, M.O.; Levtn, M.; De Oliveira, S.F.; Dos Santos Costa, P.C.; Bergami, P.L.; Dos Santos Almeida, N.A.; Pedrosa, R.C.; Ferrari, I.; Hoebeke, J.; De Carvalho, A.C.C. "Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens" (1998) FASEB Journal. 12(14):1551-1558
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Abstract:

Antibodies of chronic chagasic patients have been shown to interfere with electric and mechanical activities of cardiac embryonic myocytes in culture and with whole mammalian hearts. A mechanism proposed for this effect involves interaction of the antibodies with G-protein-linked membrane receptors, thus leading to activation of beta adrenergic and muscarinic receptors; more specifically, IgG of chagasic patients would interact with the negatively charged regions of the second extracellular loop of these receptors. We performed competition experiments to test this hypothesis. We evaluated the effect of sera/IgG from patients previously known to depress electrogenesis and/or atrioventricular conduction in isolated rabbit hearts after incubation with live and lysed parasites, the peptide corresponding to the second extracellular loop (O2) of the M2 receptor, and different peptides derived from two ribosomal proteins of T. cruzi: P0 and P2β. Our results indicate that 1) the antigenic factor inducing the functionally active IgGs in the chagasic patients is probably an intracellular T. cruzi antigen; 2) IgG/serum is interacting with the O2 region of the M2 receptor in the rabbit heart; and 3) the negative charges present in the ribosomal proteins of T. cruzi are important in mediating the interaction between the patients' serum/IgG and the receptor.

Registro:

Documento: Artículo
Título:Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens
Autor:Masuda, M.O.; Levtn, M.; De Oliveira, S.F.; Dos Santos Costa, P.C.; Bergami, P.L.; Dos Santos Almeida, N.A.; Pedrosa, R.C.; Ferrari, I.; Hoebeke, J.; De Carvalho, A.C.C.
Filiación:Inst. de Biofis. Carlos Chagas Filho, CCS, UFRJ, Rio de Janeiro, RJ, Brazil
Hosp. Univ. Clementin Fraga Filho, CCS, UFRJ, Rio de Janeiro, RJ, Brazil
Inst. de Invest. en Ing. Genet. y B., 1428 Buenos Aires, Argentina
Lab. d'Enzymol. et de Chim. de P., Ctr. Natl. de la Rech. Scientifique, F-37032 Tours, France
Inst. de Biofis. Carlos Chagas Filho, CCS, bloco G, Ilha do Funclão, 21949-900 Rio de Janeiro, RJ, Brazil
Palabras clave:Heart block; Immunoglobulin; Muscarinic receptor; Ribosomal P proteins; guanine nucleotide binding protein; ribosome protein; animal tissue; antigen specificity; article; atrioventricular conduction; cell interaction; chagas disease; chronic disease; gene targeting; heart block; nonhuman; pathogenesis; priority journal; protein isolation; rabbit; trypanosoma cruzi; Amino Acid Sequence; Animals; Antigens, Protozoan; Autoantibodies; Chagas Disease; Cross Reactions; Electrocardiography; Heart; Molecular Sequence Data; Myocardium; Rabbits; Receptors, Muscarinic; Ribosomal Proteins; Trypanosoma cruzi; Animalia; Mammalia; Oryctolagus cuniculus; Trypanosoma; Trypanosoma cruzi
Año:1998
Volumen:12
Número:14
Página de inicio:1551
Página de fin:1558
Título revista:FASEB Journal
Título revista abreviado:FASEB J.
ISSN:08926638
CODEN:FAJOE
CAS:Antigens, Protozoan; Autoantibodies; Receptors, Muscarinic; Ribosomal Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda

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Citas:

---------- APA ----------
Masuda, M.O., Levtn, M., De Oliveira, S.F., Dos Santos Costa, P.C., Bergami, P.L., Dos Santos Almeida, N.A., Pedrosa, R.C.,..., De Carvalho, A.C.C. (1998) . Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens. FASEB Journal, 12(14), 1551-1558.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda [ ]
---------- CHICAGO ----------
Masuda, M.O., Levtn, M., De Oliveira, S.F., Dos Santos Costa, P.C., Bergami, P.L., Dos Santos Almeida, N.A., et al. "Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens" . FASEB Journal 12, no. 14 (1998) : 1551-1558.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda [ ]
---------- MLA ----------
Masuda, M.O., Levtn, M., De Oliveira, S.F., Dos Santos Costa, P.C., Bergami, P.L., Dos Santos Almeida, N.A., et al. "Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens" . FASEB Journal, vol. 12, no. 14, 1998, pp. 1551-1558.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda [ ]
---------- VANCOUVER ----------
Masuda, M.O., Levtn, M., De Oliveira, S.F., Dos Santos Costa, P.C., Bergami, P.L., Dos Santos Almeida, N.A., et al. Functionally active cardiac antibodies in chronic Chagas' disease are specifically blocked by Trypanosoma cruzi antigens. FASEB J. 1998;12(14):1551-1558.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v12_n14_p1551_Masuda [ ]