Artículo
Abstract:
With the aim to characterize the drastic decrease of Uroporphyrinogen Decarboxylase (Uro-D)activity produced in experimental polyhalogenated aromatic hydrocarbons porphyria, we began to study normal rat liver Uro-D. In order to purify the enzyme, a liver homogenate was subjected to the following purification scheme: centrifugation 20 min at 11,000 x g, supernatant ammonium sulphate precipitation (35-70% sat), phosphate gel absorption, affinity chromatography and gel filtration. We found a complex mixture of proteins exhibiting Uro-D activity. At first we detected two isoforms with MW of 45,000 and 30,000 (IfA and IfB respectively). Trying to further purify IfA by re-cromatography on a G-75 column, we detected a third form of MW 17,000, which appeared in a molecular ratio of about 1:1 respect to IfB. We started the characterization of IfA and IfB, obteining a Km of 0.27 #M and 0.55 p.M for IrA and 5.0 #M and 0.5 #M for IfB, using Uroporphyrinogen III or Pentacarboxyporphyrinogen III as substrate respectively. Besides, we asseyed 2 carboxylic group reagents which showed to have an inhibiting effect on both isoforms, meaning that in the enzymes a carboxyl group would be involved in their active center. This is the first report about 3 hepatic proteins that show Uro-D activity.
Registro:
| Documento: | Artículo |
| Título: | Detection and partial characterization of three isoforms of rat liver uroporphyrinogen decarboxylase |
| Autor: | Corvi, M.; Guidi, S.; Chaufan, G.; De San Martin Viale, L.; De Rios Molina, M.C. |
| Filiación: | Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Año: | 1997 |
| Volumen: | 11 |
| Número: | 9 |
| Título revista: | FASEB Journal |
| Título revista abreviado: | FASEB J. |
| ISSN: | 08926638 |
| CODEN: | FAJOE |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v11_n9_p_Corvi |
Citas:
---------- APA ----------
Corvi, M., Guidi, S., Chaufan, G., De San Martin Viale, L. & De Rios Molina, M.C. (1997) . Detection and partial characterization of three isoforms of rat liver uroporphyrinogen decarboxylase. FASEB Journal, 11(9).Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v11_n9_p_Corvi [ ]
---------- CHICAGO ----------
Corvi, M., Guidi, S., Chaufan, G., De San Martin Viale, L., De Rios Molina, M.C. "Detection and partial characterization of three isoforms of rat liver uroporphyrinogen decarboxylase" . FASEB Journal 11, no. 9 (1997).Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v11_n9_p_Corvi [ ]
---------- MLA ----------
Corvi, M., Guidi, S., Chaufan, G., De San Martin Viale, L., De Rios Molina, M.C. "Detection and partial characterization of three isoforms of rat liver uroporphyrinogen decarboxylase" . FASEB Journal, vol. 11, no. 9, 1997.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v11_n9_p_Corvi [ ]
---------- VANCOUVER ----------
Corvi, M., Guidi, S., Chaufan, G., De San Martin Viale, L., De Rios Molina, M.C. Detection and partial characterization of three isoforms of rat liver uroporphyrinogen decarboxylase. FASEB J. 1997;11(9).Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_08926638_v11_n9_p_Corvi [ ]
